Abstract: Ischemic events can culminate in acute myocardial infarction with irreversible cardiac lesions that cannot be restored due to the limited regenerative capacity of the heart. Tissue engineering proposes therapeutic alternatives by using biomaterials to resemble the native extracellular medium combined with healthy and functional cells. This research focused on developing a natural thermosensitive hydrogel, its physical-chemical characterization and in vitro biocompatibility determination. Hydrogels’ morphological characterization was carried out through scanning electron microscopy and its chemical characterization by employing Infrared Spectroscopy technic. In addition, the biocompatibility was determined using fetal human ventricular cardiomyocytes cell line RL-14 and the MTT cytotoxicity test according to the ISO 10993-5 standard. Four biocompatible and thermosensitive hydrogels were obtained with a three-dimensional internal structure and two gelation times. The results show the potential of the hydrogel to increase the cell survival rate to the cardiac cell therapies under investigation and lay the foundations to continue with its characterization and biological evaluation both in vitro and in vivo models.
Abstract: Composting is the process in which municipal solid
waste (MSW) and other organic waste materials such as biosolids
and manures are decomposed through the action of bacteria and other
microorganisms into a stable granular material which, applied to
land, as soil conditioner. Microorganisms, especially those that are
able to degrade polymeric organic material have a key role in speed
up this process. The aim of this study has been established to
isolation of microorganisms with high ability to production
extracellular enzymes for degradation of natural polymers that are
exists in MSW for decreasing time of degradation phase. Our
experimental study for isolation designed in two phases: in first
phase we isolated degrading microorganism with selected media that
consist a special natural polymer such as cellulose, starch, lipids and
etc as sole source of carbon. In second phase we selected
microorganism that had high degrading enzyme production with
enzymatic assay for seed production. However, our findings in pilot
scale have indicated that usage of this microbial consortium had high
efficiency for decreasing degradation phase.