Abstract: An experiment was conducted under controlled
conditions to study the effect of water extract of leaves, shoots and
roots of either Sisymbrium irio L. =SISIR and/or Descurainia
sophia (L.) Schur =DESSO on the germination and primary growth
of wheat. A split-split plot experiment in CRD with three
replications was used. The main plots were the type of weed: i.e.
SISIR and DESSO and the sub-plots were type of organ: i.e. leaf,
stem and root and, the sub-sub plots were concentration of the
water extract of each organ of the weeds: i.e. 0, 2, 4 and 8 % w/v.
The plant materials were cut in 2-3 cm pieces and then were
ground in a blender. The crushed materials were weighed
according to experimental protocol and the final volume was
reached to 100 ml in distilled water in dark bottles. All bottles were
put on a shaker for 24 hours. The solutions were filtered by muslin
cloth. Whatman paper, 9 cm in diameter, were put in petri dishes
and twenty seeds of wheat were put on it and 5 ml distilled water
or water extract of weeds were added to each petri dish. All petri
dishes were put in constant temperature of 15 0C incubator.
The results showed that the SISIR water extract had a greater
inhibitory effects on germination and primary growth of wheat
than those of DESSO water extract. The water extracts of the
leaves of both weeds had the greatest inhibitory effects on
germination and primary growth of wheat, compared to those of
stems and roots. Increasing the concentration of water extract of
leaves, stems and roots of both weeds up to 8 % caused the greatest
inhibitory effects to wheat and reduced the germination rate and
primary growth of it linearly.
Abstract: To study on effect of PEG and NaCl stress on
germination and early seedling stages on two cultivar of corn, two
separated experiment were laid out at physiology laboratory, faculty
of Agriculture, Razi University, Kermanshah, Iran in 2009. This
investigation was performed as factorial experiment under Complete
Randomized Design (CRD) with three replications. Cultivar factor
contains of two varieties (sweet corn SC403 and Flint corn SC704)
and five levels of stress (0, -2, -4, -6 and -8 bar). The principal aim of
current study was to compare the two varieties of maize in relative to
the stress conditions. Results indicated that significant decrease was
observed in percentage of germination, germination rate, length of
radicle and plumule and radicle and plumule dry matter. On the basis
of the results, NaCl as compared with PEG had more effect on
germination and early seedling stage and sweet corn had more
resistant than flint corn in both stress conditions.
Abstract: Malting is usually carried out on intact barley seed,
while hull is still attached to it. In this study, oat grain with and
without hull was subjected to controlled germination to optimize its
enzymes activity, in such a way that lipase has the lowest and α-
amylase and proteinase the highest activities. Since pH has a great
impact on the activity of the enzymes, the pH of germination media
was set up to 3 to 8. In dehulled oats, lipase and α-amylase had the
lowest and highest activities in pHs 3 and 6, respectively whereas the
highest proteinase activity was evidenced at pH 7 and 4 in the oats
with and without hull respectively. While measurements indicated
that the effect of hull on the enzyme activities particularly in lipase
and amylase at each level of the pH are significantly different, the
best results were obtained in those samples in which their hull had
been removed. However, since the similar lipase activity in
germinated dehulled oat were recorded at the pHs 4 and 5, therefore
it was concluded that pH 5 in dehulled oat seed may provide the
optimum enzyme activity for all the enzymes.
Abstract: In production of medicinal plants, seed germination is
very important problem. The treated seeds (control, hydro priming
and ZnSO4) of Cumin (Cuminum cyminum L.) were evaluated at
germination and seedling growth for tolerance to salt (NaCl and
Na2SO4) conditions at the same water potentials of 0.0, -0.3, -0.6, -
0.9 and -1.2MPa. Electrical conductivity (EC) values of the NaCl
solutions were 0.0, 6.5, 12.7, 18.4 and 23.5 dSm-1, respectively. The
objective of the study was to determine factors responsible for
germination and early seedling growth due to salt toxicity or osmotic
effect and to optimize the best priming treatment for these stress
conditions. Results revealed that germination delayed in both
solutions, having variable germination with different priming
treatments. Germination, shoot and weight, root and shoot length
were higher but mean germination time and abnormal germination
percentage were lower in NaCl than Na2SO4 at the same water
potential. The root / shoot weight and R/S length increased with
increase in osmotic potential in both NaCl and Na2SO4 solutions.
NaCl had less inhibitor effect on seedling growth than the
germination. It was concluded that inhibition of germination at the
same water potential of NaCl and Na2SO4 resulted from salt toxicity
rather than osmotic effect. Hydro priming increased germination and
seedling growth under salt stress. This protocol has practical
importance and could be recommended to farmers to achieve higher
germination and uniform emergence under field conditions.
Abstract: Pentachlorophenol (PCP) is a polychlorinated
aromatic compound that is widespread in industrial effluents and is
considered to be a serious pollutant. Among the variety of industrial
effluents encountered, effluents from tanning industry are very
important and have a serious pollution potential. PCP is also formed
unintentionally in effluents of paper and pulp industries. It is highly
persistent in soils and is lethal to a wide variety of beneficial
microorganisms and insects, human beings and animals. The natural
processes that breakdown toxic chemicals in the environment have
become the focus of much attention to develop safe and environmentfriendly
deactivation technologies. Microbes and plants are among
the most important biological agents that remove and degrade waste
materials to enable their recycling in the environment. The present
investigation was carried out with the aim of developing a microbial
system for bioremediation of PCP polluted soils. A number of plant
species were evaluated for their ability to tolerate different
concentrations of pentachlorophenol (PCP) in the soil. The
experiment was conducted for 30 days under pot culture conditions.
The toxic effect of PCP on plants was studied by monitoring seed
germination, plant growth and biomass. As the concentration of PCP
was increased to 50 ppm, the inhibition of seed germination, plant
growth and biomass was also increased. Although PCP had a
negative effect on all plant species tested, maize and groundnut
showed the maximum tolerance to PCP. Other tolerating crops
included wheat, safflower, sunflower, and soybean. From the
rhizosphere soil of the tolerant seedlings, as many as twenty seven
PCP tolerant bacteria were isolated. From soybean, 8; sunflower, 3;
safflower 8; maize 2; groundnut and wheat, 3 each isolates were
made. They were screened for their PCP degradation potentials.
HPLC analyses of PCP degradation revealed that the isolate MAZ-2
degraded PCP completely. The isolate MAZ-1 was the next best
isolate with 90 per cent PCP degradation. These strains hold promise
to be used in the bioremediation of PCP polluted soils.
Abstract: The paper presents the influence of the conventional
ploughing tillage technology in comparison with the minimum
tillage, upon the soil properties, weed control and yield in the case of
maize (Zea mays L.), soya-bean (Glycine hispida L.) and winter
wheat (Triticum aestivum L.) in a three years crop rotation. A
research has been conducted at the University of Agricultural
Sciences and Veterinary Medicine Cluj-Napoca, Romania. The use of
minimum soil tillage systems within a three years rotation: maize,
soya-bean, wheat favorites the rise of the aggregates hydro stability
with 5.6-7.5% on a 0-20 cm depth and 5-11% on 20-30 cm depth.
The minimum soil tillage systems – paraplow, chisel or rotary grape
– are polyvalent alternatives for basic preparation, germination bed
preparation and sowing, for fields and crops with moderate loose
requirements being optimized technologies for: soil natural fertility
activation and rationalization, reduction of erosion, increasing the
accumulation capacity for water and realization of sowing in the
optimal period. The soil tillage system influences the productivity
elements of cultivated species and finally the productions thus
obtained. Thus, related to conventional working system, the
productions registered in minimum tillage working represented 89-
97% in maize, 103-112% in soya-bean, 93-99% in winter-wheat. The
results of investigations showed that the yield is a conclusion soil
tillage systems influence on soil properties, plant density assurance
and on weed control. Under minimum tillage systems in the case of
winter weat as an option for replacing classic ploughing, the best
results in terms of quality indices were obtained from version worked
with paraplow, followed by rotary harrow and chisel. At variants
worked with paraplow were obtained quality indices close to those of
the variant worked with plow, and protein and gluten content was
even higher. At Ariesan variety, highest protein content, 12.50% and
gluten, 28.6% was obtained for the variant paraplow.
Abstract: Acid rain occurs when sulphur dioxide (SO2) and
nitrogen oxides (Nox) gases react in the atmosphere with water,
oxygen, and other chemicals to form various acidic compounds. The
result is a mild solution of sulfuric acid and nitric acid. Soil has a
greater buffering capacity than aquatic systems. However excessive
amount of acids introduced by acid rains may disturb the entire soil
chemistry. Acidity and harmful action of toxic elements damage
vegetation while susceptible microbial species are eliminated. In
present study, the effects of simulated sulphuric acid and nitric acid
rains were investigated on crop Glycine max. The effect of acid rain
on change in soil fertility was detected in which pH of control sample
was 6.5 and pH of 1%H2SO4 and 1%HNO3 were 3.5. Nitrogen nitrate
in soil was high in 1% HNO3 treated soil & Control sample.
Ammonium nitrogen in soil was low in 1% HNO3 & H2SO4 treated
soil. Ammonium nitrogen was medium in control and other samples.
The effect of acid rain on seed germination on 3rd day of germination
control sample growth was 7 cm, 0.1% HNO3 was 8cm, and 0.001%
HNO3 & 0.001% H2SO4 was 6cm each. On 10th day fungal growth
was observed in 1% and 0.1%H2SO4 concentrations, when all plants
were dead. The effect of acid rain on crop productivity was
investigated on 3rd day roots were developed in plants. On12th day
Glycine max showed more growth in 0.1% HNO3, 0.001% HNO3 and
0.001% H2SO4 treated plants growth were same as compare to control
plants. On 20th day development of discoloration of plant pigments
were observed on acid treated plants leaves. On 38th day, 0.1, 0.001%
HNO3 and 0.1, 0.001% H2SO4 treated plants and control plants were
showing flower growth. On 42th day, acid treated Glycine max variety
and control plants were showed seeds on plants. In Glycine max
variety 0.1, 0.001% H2SO4, 0.1, 0.001% HNO3 treated plants were
dead on 46th day and fungal growth was observed. The toxicological
study was carried out on Glycine max plants exposed to 1% HNO3
cells were damaged more than 1% H2SO4. Leaf sections exposed to
0.001% HNO3 & H2SO4 showed less damaged of cells and
pigmentation observed in entire slide when compare with control
plant. The soil analysis was done to find microorganisms in HNO3 &
H2SO4 treated Glycine max and control plants. No microorganism
growth was observed in 1% HNO3 & H2SO4 but control plant showed
microbial growth.
Abstract: The spores of entomopathogenic fungi, Beauveria bassiana was evaluated for their compatibility with four surfactants; SDS (sodium dodyl sulphate) and CABS-65 (calcium alkyl benzene sulphonate), Tween 20 (polyethylene sorbitan monolaureate) and Tween 80 (polyoxyethylene sorbitan monoleate) at six different concentrations (0.1%, 0.5%, 1%, 2.5%, 5% and 10%). Incubated spores showed decrease in concentrations due to conversion of spores to hyphae. The maximum germination recorded in 72 h incubated spores varied with surfactant concentration at 49-68% (SDS), 39- 53% (CABS), 78-92% (Tween 80) and 80-92% (Tween 20), while the optimal surfactant concentration for spore germination was found to be 2.5-5%. The surfactant effect on spores was more pronounced with SDS and CABS-65, where significant deterioration and loss in viability of the incubated spores was observed. The effect of Tween 20 and Tween 80 were comparatively less inhibiting. The results of the study would help in surfactant selection for B. bassiana emulsion preparation.
Abstract: The evaluation of non-conventional water resources
on seed germination and seedling growth performance at early
growth stages is still in progress especially in forage crops. This
study was designed to test the effect of four types of water qualities
(treated wastewater (TWW), industrial water (IW), grey water (GW),
and Distilled water (DW)) on germination and early seedling vigor of
Leucaena leucocephala. The results showed that the germination
was not significantly affected by the different water qualities. Seed
germination reached maximum after 17, 14, 14, and 21 days under
GW, IW, TWW, and DW treatments, respectively. The highest mean
of shoot length was scored under the GW treatment. And, the highest
mean of root length was scored under DW which was not significant
from GW treatment. The means of shoot fresh was the highest under
the TWW. The means of root fresh weight was not significantly
different from each other's under different treatments. The growth
performance was in progress with no mortality during 21 days of
growth. Thus, the best non-conventional water qualities alternatives
based on the cleanness, nutrients, and toxicity are the GW, TWW and
IW, respectively.
Abstract: A study was undertaken to investigate the effect of
liquid nitrogen aeration on mortalities of adult Cryptolestes
furrugineus, rusty grain beetles, in a prototype cardboard grain bin
equipped with an aeration system. The grain bin was filled with Hard
Red Spring wheat and liquid nitrogen was introduced from the bottom
of the bin. The survival of both cold acclimated and unacclimated C.
furrugineus was tested. The study reveals that cold acclimated insects
had higher survival than unacclimated insects under similar cooling
conditions. In most cases, mortalities of as high as 100% were
achieved at the bottom 100 cm of the grain bin for unacclimated
insects for most of the trials. Insect survival increased as the distance
from the bottom of the grain bin increased. There was no adverse
effect of liquid nitrogen aeration on wheat germination.
Abstract: A simple mobile engine-driven pneumatic paddy
collector made of locally available materials using local
manufacturing technology was designed, fabricated, and tested for
collecting and bagging of paddy dried on concrete pavement. The
pneumatic paddy collector had the following major components:
radial flat bladed type centrifugal fan, power transmission system,
bagging area, frame and the conveyance system. Results showed
significant differences on the collecting capacity, noise level, and fuel
consumption when rotational speed of the air mover shaft was varied.
Other parameters such as collecting efficiency, air velocity,
augmented cracked grain percentage, and germination rate were not
significantly affected by varying rotational speed of the air mover
shaft. The pneumatic paddy collector had a collecting efficiency of
99.33 % with a collecting capacity of 2685.00 kg/h at maximum
rotational speed of centrifugal fan shaft of about 4200 rpm. The
machine entailed an investment cost of P 62,829.25. The break-even
weight of paddy was 510,606.75 kg/yr at a collecting cost of 0.11
P/kg of paddy. Utilizing the machine for 400 hours per year
generated an income of P 23,887.73. The projected time needed to
recover cost of the machine based on 2685 kg/h collecting capacity
was 2.63 year.
Abstract: This study investigated the ecological effects of
particulate pollution from a cement factory on the vegetation in the
western Mediterranean coastal desert of Egypt. Variations in
vegetation, soil chemical characters, and some responses of Atriplex
halimus, as a dominant species in the study area, were investigated in
some sites located in different directions from the cement factory
between Burg El-Arab in the east and El-Hammam in the west. The
results showed an obvious decrease in vegetation diversity, in
response to cement-kiln dust pollution, that accompanied by a high
dominance attributed to the high contribution of Atriplex halimus.
Annual species were found to be more sensitive to cement dust
pollution as they all failed to persist in highly disturbed sites. It is
remarkable that cover and phytomass of Atriplex halimus were
increased greatly in response to cement dust pollution, and this was
accompanied by a reduction in the mature seeds and leaf-area of the
plant. The few seeds of the affected individuals seemed to be more
fertile and attained higher germination percentages and exhibited
hardening against drought stress.
Abstract: Bacillus subtilis strain LB5 produced lipopeptide
antibiotic iturin A-2 in liquid medium. Crude extract
from cell-free supernatant of B. subtilis cultivated broth extracted
with n-butanol showed antifungal activity to conidial germination of
Colletotrichum gloeosporioides. The germination of conidia was
completely inhibited by crude extract. The ultrastructure of conidia
after treated with crude extract was found an accumulation of vesiclelike
material between cell wall and plasma membrane while this
accumulation was not observed in untreated and germinated conidia.
Besides, the cell wall was not affected by crude extract.
Abstract: The heterotrophic seedling growth can be defined as a
product of two components: (1) the weight of mobilized seed reserve,
and (2) conversion efficiency of utilized seed reserve to seedling
tissue. The first component can be further divided into (1) initial seed
weight, and (2) the fraction of seed reserve, which is mobilized. The
objective of this study was the identification of the sensitive seedling
growth component(s) in response to drought and salinity stresses.
Two experiments were separately conducted using various salinity
levels (osmotic pressure) of 0, 0.25, 0.50, 0.75, 1, 1.25 and 1.5 MPa
created using NaCl as first experiment and by polyethylene glycol
(drought stress) of 0, 0.2, 0.4, 0.6, 0.8, 1, 1.2 and 1.4 MPa in second
experiment. Seeds of five crops species (Hordeum vulgare, Brassica
napus, Zea mays, Medicago sativa and Medicago scutellata) were
used in each experiment. In both experiments, seedling growth,
fraction of seed reserve utilization and weight of mobilized seed
reserve decreased with increasing drought and salt intensity.
However, drought and salinity stresses had no effect on the
conversion efficiency. It was concluded that the sensitive component
of seedling growth is the weight of mobilized seed reserve.
Abstract: In the present study, the effects of ultrasound as
emerging technology were investigated on germination stimulation,
amount of alpha-amylase activity on dry barley seeds before steeping
stage of malting process. All experiments were carried out at 20 KHz
on the ultrasonic generator in 3 different ultrasonic intensities (20, 60
and 100% setting from total power of device) and time (5, 10 and 15
min) at constant temperature (30C). For determining the effects of
these parameters on enzyme the Fuwa method assay based on the
decreased staining value of blue starch–iodine complexes employed
for measurement an activity. The results of these assays were
analyzed by Qualitek4 software using the Taguchi statistical method
to evaluate the factor-s effects on enzyme activity. It has been found
that when malting barley is irradiated with an ultrasonic power, a
stimulating effect occurs as to the enzyme activity.
Abstract: One problem of synthetic sunflower cultivation is an
erratic germination of the seeds. To improve the germination, presowing
seed treatment with an ultrasound was tested. All treatments
were carried out at 40 kHz frequency with the intensities of 40, 60,
80 and 100% of the ultrasonic generator total power (250 W) for the
durations of 5, 10, 15 and 20 minutes. Data on seed germination
percentage, seed vigor index (SVI), root and shoot lengths of
seedlings were collected. The results showed that germination, SVI,
root and shoot lengths of ultrasonic treated seedlings were different
from the control, depending on intensity of the ultrasound. The
effects of ultrasonic treatment were significant on germination,
resulting in a maximum increase of 43% at 40 and 60% intensities
compared to that of the control seeds. In addition, seedlings of these 2
treatments had higher SVI and longer root and shoot lengths than that
of the control seedlings. All treatment durations resulted in higher
germination and SVI, longer root and higher shoot lenghts of
seedlings than the control. Among the duration treatments, only SVI
and seedling root length were significantly different.
Abstract: The effect of antifungal compound from Bacillus
subtilis strain LB5 was tested against conidial germination of
Colletotrichum gloeosporioides and Pestalotiopsis eugeniae, causal
agent of anthracnose and fruit rot of wax apple, respectively.
Observation under scanning electron microscope and light compound
microscope revealed that conidial germination was completely
inhibited when treated with culture broth, culture filtrate, or crude
extract from strain LB5. Identification of purified antifungal
compound produced by strain LB5 in cell-free supernatant by nuclear
magnetic resonance and fast atom bombardment showed that the
active compound was iturin A-2.
Abstract: Biologically active peptides are of particular interest
in food science and human nutrition because they have been shown to play several physiological roles. In vitro gastrointestinal digestion of lentil and whey proteins in this study produced high angiotensin-I converting enzyme inhibitory activity with 75.5±1.9 and 91.4±2.3%
inhibition, respectively. High ACE inhibitory activity was observed in lentil after 5 days of germination (84.3±1.2%). Fractionation by
reverse phase chromatography gave inhibitory activities as high as
86.3±2.0 for lentil, 94.8±1.8% for whey and 93.7±1.7% at 5th day of germination. Further purification by HPLC resulted in several
inhibitory peptides with IC50 values ranging from 0.064 to 0.164
mg/ml. These results demonstrate that lentil proteins are a good
source of peptides with ACE inhibitory activity that can be released by germination or gastrointestinal digestion. Despite the lower bioactivity in comparison with whey proteins, incorporation of lentil proteins in functional food formulations and natural drugs look promising.