Abstract: Alongside with antioxidant, pro-oxidant activity is also observed in phytochemical compounds. In the study, Ficus odorata, an endemic medicinal plant in the Philippines, was screened for the potential medical application of its pro-oxidant activity.
Phytochemical screening revealed the presence of terpenes, glycosides and phenolic acids. The crude extract was found to contain low gallic acid and quercetin equivalence. The TLC chromatogram of the crude extract showed that none of the 11 spots obtained has antioxidant activity nor correspond to gallic acid and quercetin standards. Experiments showed that the crude extract has stimulatory activity towards DPPH radicals, hydrogen peroxide, hydroxyl radicals, superoxide anions and nitric oxide. Moreover, the extract exhibited a low ferric reducing power.
The prooxidant activity was evident in the crude ethanolic leaf extract of F. odorata, which may provide a better understanding of the plant’s pharmacological importance in the prevention of diseases.
Abstract: This study was conducted to evaluate the anti-diabetic
properties of ethanolic extract of two plants commonly used in folk
medicine, Mormodica charantia (bitter melon) and Trigonella
foenum-graecum (fenugreek). The study was performed on STZinduced
diabetic rats (DM type-I). Plant extracts of these two plants
were given to STZ diabetic rats at the concentration of 500 mg/kg
body weight ,50 mg/kg body weight respectively. Cidophage®
(metformin HCl) were administered to another group to support the
results at a dose of 500 mg/kg body weight, the ethanolic extracts and
Cidophage administered orally once a day for four weeks using a
stomach tube and; serum samples were obtained for biochemical
analysis. The extracts caused significant decreases in glucose levels
compared with diabetic control rats. Insulin secretions were increased
after 4 weeks of treatment with Cidophage® compared with the
control non-diabetic rats. Levels of AST and ALT liver enzymes were
normalized by all treatments. Decreases in liver cholesterol,
triglycerides, and LDL in diabetic rats were observed with all
treatments. HDL levels were increased by the treatments in the
following order: bitter melon, Cidophage®, and fenugreek. Creatinine
levels were reduced by all treatments. Serum nitric oxide and
malonaldehyde levels were reduced by all extracts. GSH levels were
increased by all extracts. Extravasation as measured by the Evans
Blue test increased significantly in STZ-induced diabetic animals.
This effect was reversed by ethanolic extracts of bitter melon or
fenugreek.
Abstract: In this study the extracts of the Iraqi herb Tribulus
terrestris (Al-Hassage or Al-Kutub) was done by using of polar and
non polar solvents, then the biological activity of these extractants
was studied in three fields, First, the antibacterial activity (in vitro)
on gram positive bacteria (Staphylococcus aureus), and gram
negative bacteria (E. coli, Proteus vulgaris, Pseudomonas
aerugiuosa, and Klebsiella), all extracts showed considerable activity
against all bacteria. Second, the effect of extracts on free serum
testosterone level in male mice (in vivo), the alcoholic, and
acetonitrilic extracts showed significant (P < 0.05) increase in free
serum testosterone level, and we found that the extracts contained
compounds with less genotoxic effects in mice germ cells. 3rd, was to
study the effect of methanolic extract of T. terrestris in diabetes
management.
Abstract: Antifungal activities of ether and methanolic extracts of volatiles oils of Nigella Sativa seeds were tested against pathogenic bacterias and fungies strains.The volatile oil were found to have significant antifungal and antibacterial activities compare to tetracycline, cefuroxime and ciprofloxacin positive controls.The ether and methanolic esxtracts were compared to each other for antifungal and antibacterial activities and ether extracts showed stonger activity than methanolic one.
Abstract: Macrophomina phaseolina is a devastating soil-borne
fungal plant pathogen that causes charcoal rot disease in many
economically important crops worldwide. So far, no registered
fungicide is available against this plant pathogen. This study was
planned to examine the antifungal activity of an allelopathic grass
Cenchrus pennisetiformis (Hochst. & Steud.) Wipff. for the
management of M. phaseolina isolated from cowpea [Vigna
unguiculata (L.) Walp.] plants suffering from charcoal rot disease.
Different parts of the plants viz. inflorescence, shoot and root were
extracted in methanol. Laboratory bioassays were carried out using
different concentrations (0, 0.5, 1.0, …, 3.0 g mL-1) of methanolic
extracts of the test allelopathic grass species to assess the antifungal
activity against the pathogen. In general, extracts of all parts of the
grass exhibited antifungal activity. All the concentrations of
methanolic extracts of shoot and root significantly reduced fungal
biomass by 20–73% and 40–80%, respectively. Methanolic shoot
extract was fractionated using n-hexane, chloroform, ethyl acetate
and n-butanol. Different concentrations of these fractions (3.125,
6.25, …, 200 mg mL-1) were analyzed for their antifungal activity.
All the concentrations of n-hexane fraction significantly reduced
fungal biomass by 15–96% over corresponding control treatments.
Higher concentrations (12.5–200 mg mL-1) of chloroform, ethyl
acetate and n-butanol also reduced the fungal biomass significantly
by 29–100%, 46–100% and 24–100%, respectively.
Abstract: Alpinia galanga is rhizome, generally known as
Greater galangal and is selected for isolation of newer constituents
accountable for various therapeutic activities. Present study is
intended to isolate glycoside from Alpinia galanga rhizomes. Alpinia
galanga methanolic extract was column chromatograph and eluted
with ethyl acetate-methanol (99:1) to isolate compound β-Sitosterol
Diarabinoside. Herein, the isolation and structural elucidation of new
compound is described. Chemical investigation of methanolic extract
of rhizomes of Alpinia galanga furnished a new compound β-
Sitosterol Diarabinoside. The IR, NMR and MASS investigations of
isolated compound confirmed its structure as β-Sitosterol
Diarabinoside, which is isolated for the first time from a medicinal
plant or any synthetic source.
Abstract: Recent years have instance that there is a invigoration
of interest in drug discovery from medicinal plants for the support of
health in all parts of the world . This study was designed to examine
the in vitro antimicrobial activities of the flowers and leaves
methanolic and ethanolic extracts of Chenopodium album L.
Chenopodium album Linn. flowers and leaves were collected from
East Esfahan, Iran. The effects of methanolic and ethanolic extracts
were tested against 4 bacterial strains by using disc,well-diffusion
method. Results showed that flowers and leaves methanolic and
ethanolic extracts of C.album don-t have any activity against the
selected bacterial strains. Our study has indicated that ,there are
effective different factors on antimicrobial properties of plant extracts
Abstract: Protective effect of ethanolic extract of polyherbal formulation (PHF) was studied on carbon tetrachloride induced liver damage on carbon tetrachloride induced liver damage. Treatment of rats with 250mg /kg body weight of ethanolic extract of PHF protected rats against carbon tetrachloride liver injury by significant lowerering 5’ nucleotidase (5’NT), Gamma Glutamyl transferase (GGT), Glutamate dehdyrogenasse (GDH) and Succinate Dehydrogenase (SDH) levels compared to control. Normalization in these enzyme levels indicates strong hepatoprotective property of PHF extract.
Abstract: In this investigation, the antibacterial effects of
ethanolic and 7:3 isopropyl –hexane mixture extracts of Zingiber
officinale were evaluated against three Gram positive bacteria, B.
cereus, S.epidermidis, S. aureus and three Gram negative bacteria, E.
coli, K.pneumonia and P.areuginosa. Utilizing paper disk diffusion
and well methods in-vitro, MIC and MBC were determined by
macrodilution. The results showed that ethanolic rhizome extract of
ginger had significantly active than Isopropyl –hexan extract. Further
work needs to be done in these extracts including fractionation to
isolate active constituents and subsequent pharmacological
evaluation.
Abstract: Plants are rich sources of bioactive compounds. In this study the photochemical screening of hexane, ethanolic and aqueous extracts of roots and latex of jojoba (Simmondsia chinensis) plant revealed the presence of saponins, tannins, alkaloids, steroids and glycosides. Ethanolic extract was found to be richer in these metabolites than hexane, aqueous extracts and latex. The extracts and latex displayed effective antimicrobial activity against Salmonella typhimurium, Bacillus cereus, Clostridium perfringens, Staphylococcus aureus, Escherichia coli, Candida albicans and Aspergillus flavus. The increase in volume of the extracts and latex caused more activity, as shown by zones of inhibition. Candida albicans growth was inhibited only by hexane extract. Jojoba latex was not effective against Candida albicans at 0.1 and 0.5 ml extracts concentration but showed 5mm zone of inhibition at (1.0 ml). Lower volume (0.1ml) of latex encouraged Aspergillus flavus growth, while at (1.00 ml) reduced its mycelial growth. Thus, jojoba root extracts and latex can be of potential natural antimicrobial agents.
Abstract: The antimicrobial, antiplasmid and cytotoxic activities of marine algae Halimeda opuntia and Sarconema filiforme were investigated. Antimicrobial bioassay against some human pathogenic bacteria and yeast were conducted using disc diffusion method. Halimeda extract exhibited antibacterial activity against six species of microrganisms, with significant inhibition against Staphylococcus aureus. While Sarconema extract was better potent as antifungal against Candida albicans. Comparative antibacterial studies showed that Halimeda extract showed equivalent or better activity as compared with commercial antibiotic when tested against Staphylococcus aureus. Further tests conducted using dilution method showed both extracts as having bacteriostatic mode of action against the tested microorganisms. Methanol extract of two species showed significant cytotoxicity (LC50
Abstract: Antioxidant activities of ethanolic extracts of Ardisia
japonica Blume., Ageartum conyzoides Linn., and Cocculus hirsutus
Linn Diels. leaves was determined qualitatively and quantitatively in
this research. 1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radical
solution was used to investigate free radical scavenging activity of
these leaves extracts. Ascorbic acid (Vitamin C) was used as the
standard. In the present investigation, it is found that all of these
extracts have remarkable antioxidant activities. The EC50 values of
these ethanolic extracts were 12.72 μg/ml for A. japonica, 15.19
μg/ml for A. conyzoides, 10.68 μg/ml for C. hirsutus respectively.
Among these Myanmar medicinal plants, C. hirsutus showed higher
antioxidant activities as well as free radical scavenging activity than
black tea (Camellia sinensis), the famous antioxidant, and A.
japonica and A. conyzoides showed a rather lower antioxidant
activity than tea extracts. According to results from bioassay with
carrot discs infected with Agrobacterium tumefaciens, all extracts
showed anti-tumor activity after 3 weeks of incubation. No gall was
detected in carrot disks treated with C. hirsutus and A. japonica
extracts in the dose of 100ppm and in carrot discs treated with A.
conyzoides extract in the dose of 1000 ppm. Therefore, the research
clearly indicates that these weedy plants of dry farm land are
exceptionally advantageous for human health.
Abstract: This study has investigated the antidiabetic and
antioxidant potential of Pseudovaria macrophylla bark extract on
streptozotocin–nicotinamide induced type 2 diabetic rats. LCMSQTOF
and NMR experiments were done to determine the chemical
composition in the methanolic bark extract. For in vivo experiments,
the STZ (60 mg/kg/b.w, 15 min after 120 mg/kg/1 nicotinamide, i.p.)
induced diabetic rats were treated with methanolic extract of
Pseuduvaria macrophylla (200 and 400 mg/kg·bw) and
glibenclamide (2.5 mg/kg) as positive control respectively.
Biochemical parameters were assayed in the blood samples of all
groups of rats. The pro-inflammatory cytokines, antioxidant status
and plasma transforming growth factor βeta-1 (TGF-β1) were
evaluated. The histological study of the pancreas was examined and
its expression level of insulin was observed by
immunohistochemistry. In addition, the expression of glucose
transporters (GLUT 1, 2 and 4) were assessed in pancreas tissue by
western blot analysis. The outcomes of the study displayed that the
bark methanol extract of Pseuduvaria macrophylla has potentially
normalized the elevated blood glucose levels and improved serum
insulin and C-peptide levels with significant increase in the
antioxidant enzyme, reduced glutathione (GSH) and decrease in the
level of lipid peroxidation (LPO). Additionally, the extract has
markedly decreased the levels of serum pro-inflammatory cytokines
and transforming growth factor beta-1 (TGF-β1). Histopathology
analysis demonstrated that Pseuduvaria macrophylla has the
potential to protect the pancreas of diabetic rats against peroxidation
damage by downregulating oxidative stress and elevated
hyperglycaemia. Furthermore, the expression of insulin protein,
GLUT-1, GLUT-2 and GLUT-4 in pancreatic cells was enhanced.
The findings of this study support the anti-diabetic claims of
Pseudovaria macrophylla bark.
Abstract: Antibacterial activity of Plumeria alba (Frangipani)
petals methanolic extracts were evaluated against Escherichia coli,
Proteus vulgaris,Staphylococcus aureus, Klebsiella pneumoniae,
Pseudomonas aeruginosa, Staphylococcus saprophyticus,
Enterococcus faecalis and Serratia marcescens by using disk
diffusion method. Concentration extracts (80 %) showed the highest
inhibition zone towards Escherichia coli (14.3 mm). Frangipani
extract also showed high antibacterial activity against
Staphylococcus saprophyticus, Proteus vulgaris and Serratia
marcescens, but not more than the zones of the positive control used.
Comparison between two broad specrum antibiotics to frangipani
extracts showed that the 80 % concentration extracts produce the
same zone of inhibition as Streptomycin. Frangipani extracts showed
no bacterial activity towards Klebsiella pneumoniae, Pseudomonas
aeruginosa and Enterococcus faecalis. There are differences in the
sensitivity of different bacteria to frangipani extracts, suggesting that
frangipani-s potency varies between these bacteria. The present
results indicate that frangipani showed significant antibacterial
activity especially to Escherichia coli.
Abstract: The aim of the study was to investigate phytochemical
properties, antimicrobial activity and cytotoxicity of Aloe vera. The
phytochemical screening of the extracts of leaves of A. vera revealed
the presence of bioactive compounds such as alkaloids, tannins,
flavonoids phenolic compounds, and etc. with absence of cyanogenic
glycosides. Three different solvents such as methanol, ethanol and
Di-Methyl sulfoxide were used to screen the antimicrobial activity of
A. vera leaves against four human clinical pathogens by agar well
diffusion method. The maximum antibacterial activities were
observed in methanol extract followed by ethanol and Di-Methyl
sulfoxide. It was also found that remarkable antibacterial activities
with methanolic and ethanolic extracts of A. vera compared with the
standard antibiotic, tetracycline that was not active against E. coli
and S. boydii and supported the view that A. vera is a potent
antimicrobial agent compared with the conventional antibiotic.
Moreover, the brine shrimps (Artemia salina) toxicity test exhibited
LC50 value was 569.52 ppm. The resulting data indicated that the A.
vera plant have less toxic effects on brine shrimp. Hence, it is
signified that Aloe vera plant extract is safe to be used as an
antimicrobial agent.
Abstract: Erwinia carotovora var. carotovora is the main cause of soft rot in potatoes. Hyphaene thebaica was studied for biocontrol of E. carotovora which inhibited growth of E. carotovora on solid medium, a comparative study of classical and ultrasound-assisted extractions of Hyphaene thebaica fruit. The use of ultrasound decreased significant the total time of treatment and increase the total amount of crude extract. The crude extract was subjected to determine the in vitro, by a bioassay technique revealed that the treatment of paper disks with ultrasound extraction of Hyphaene thebaica reduced the growth of pathogen and produced inhibition zones up to 38mm in diameter. The antioxidant activity of ultrasound-ethanolic extract of Doum fruits (Hyphaene thebaica) was determined. Data obtained showed that the extract contains the secondary metabolites such as Tannins, Saponin, Flavonoids, Phenols, Steroids, Terpenoids, Glycosides and Alkaloids.
Abstract: The methanolic extracts from seeds of tamarind
(Tamarindus indica) was prepared by Soxhlet apparatus extraction
and evaluated for total phenolic content by Folin-Ciocalteu method.
Then, methanolic extract was screened biological activities (In vitro)
for anti-melanogenic activity by tyrosinase inhibition test, antiinflammation
activity by cyclooxygenase 1 (COX-1) and
cyclooxygenase 2 (COX-2) inhibition test, and cytotoxic screening
test with Vero cells. The results showed that total phenolic content,
which contained in extract, was contained 27.72 mg of gallic acid
equivalent per g of dry weight. The ability to inhibit tyrosinase
enzyme, which exerted by Tamarind seed extracts (1 mg/ml) was
52.13 ± 0.42 %. The extract was not possessed inhibitory effect to
COX-1 and COX-2 enzymes and cytotoxic effect to Vero cells. The
finding is concludes that tested seed extract was possessed
antimelanogenic activity with non-toxic effects. However, there was
not exhibited anti-inflammatory activity. Further studies include the
use of advance biological models to confirm this biological activity,
as well as, the isolation and characterization of the purified
compounds that it was contained.
Abstract: In this study, the forty Thai medicinal plants were
used to screen the antibacterial activity against Campylobacter jejuni.
Crude 95% ethanolic extracts of each plant were prepared.
Antibacterial activity was investigated by the disc diffusion assay,
and MICs and MBCs were determined by broth microdilution. The
results of antibacterial screening showed that five plants have activity
against C.jejuni including Adenanthera pavonina L., Moringa
oleifera Lam., Annona squamosa L., Hibiscus sabdariffa L. and
Eupotorium odortum L. The extraction of A. pavonina L. and A.
squamosa L. produced an outstanding against C. jejuni, inhibiting
growth at 62.5-125 and 250-500 μg/mL, respectively. The MBCs of
two extracts were just 4-fold higher than MICs against C. jejuni,
suggesting the extracts are bactericidal against this species. These
results indicate that A. pavonina and A. squamosa could potentially
be used in modern applications aimed at treatment or prevention of
foodborne disease from C. jejuni.
Abstract: Nigella sativa L. is an aromatic plant belonging to the
family Ranunculaceae. It has been used traditionally, especially in the
middle East and India, for the treatment of asthma, cough, bronchitis,
headache, rheumatism, fever, influenza and eczema. Several
biological activities have been reported in Nigella sativa seeds,
including antioxidant. In this context we tried to estimate the
antioxidant activity of various extracts prepared from Nigella sativa
seeds, methanolic extract (ME), chloroformic extract (CE), hexanic
extract (HE : fixed oil), ethyl acetate extract (EAE) water extract
(WE). The Folin-Ciocalteu assay showed that CE and EAE contained
high level of phenolic compounds 81.31 and 72.43μg GAE/mg of
extract respectively. Similarly, the CE and EAE exhibited the highest
DPPH radical scavenging activity, with IC50 values of 106.56μg/ml
and 121.62μg/ml respectively. In addition, CE and HE showed the
most scavenging activity against superoxide radical generated in the
PMS-NADH-NBT system with respective IC50 values of 361.86
μg/ml and 371.80 μg/ml, which is comparable to the activity of the
standard antioxidant BHT (344.59 μg/ml). Ferrous ion chelating
capacity assay showed that WE, EAE and ME are the most active
with 40.57, 39.70 and 22.02 mg EDTA-E/g of extract. The inhibition
of linoleic acid/ß-carotene coupled oxidation was estimated by ßcarotene
bleaching assay, this showed a highest relative antioxidant
activity with CE and EAE (69.82% of inhibition). The antioxidant
activities of the methanolic extract and the fixed oil are confirmed by
an in vivo assay in mice, the daily oral administration of methanolic
extract (500 and 800 mg/kg/day) and fixed oil (2 and 4 ml/kg/day)
during 21 days, resulted in a significant enhancement of the blood
total antioxidant capacity (measured by KRL test) and the plasmatic
antioxidant capacity towards DPPH radical.
Abstract: The present research was designed to investigate the
anti-microbial activity of aristolochic acid from the root of
Aristolochia bracteata. From the methanolic & ethyl extract extracts
of Aristolochia bracteata aristolochic acid I was isolated and
conformed through IR, NMR & MS. The percentage purity of
aristolochic acid I was determined by UV & HPLC method. Antibacterial
activity of extracts of Aristolochia bracteata and the
isolated compound was determined by disc diffusion method. The
results reveled that the isolated aristolochic acid from methanolic
extract was more pure than the compound from ethyl acetate extract.
The various extracts (500μg/disc) of Aristolochia bracteata showed
moderate antibacterial activity with the average zone of inhibition of
7-18 mm by disc diffusion method. Among the extracts, ethyl acetate
& methanol extracts were shown good anti-microbial activity and the
growth of E.coli (18 mm) was strongly inhibited. Microbial assay of
isolated compound (Aristolochic acid I) from ethyl acetate &
methanol extracts were shown good antimicrobial activity and the
zone of inhibition of both at higher concentration 50 μg/ml was
similar with the standard aristolochic acid. It may be concluded that
the isolated compound of aristolochic acid I has good anti-bacterial
activity.