Abstract: The toxicity of bare zinc oxide nanoparticles used as drug carriers may be the result of releasing zinc ions. Thus, zinc oxide nanoparticles modified with galactose were obtained. The process of their formation was conducted in the microwave field. The physicochemical properties of the obtained products were studied. The size and electrokinetic potential were defined by using dynamic light scattering technique. The crystalline properties were assessed by X-ray diffractometry. In order to confirm the formation of the desired products, Fourier-transform infrared spectroscopy was used. Releasing of zinc ions from the prepared products when comparing to the bare oxide was analyzed. It was found out that modification of zinc oxide nanoparticles with galactose limits the releasing of zinc ions which are responsible for the toxic effect of the whole carrier-drug conjugate.
Abstract: The paper presents a process to obtain glutathione-modified titanium oxide nanoparticles. The processes were carried out in a microwave radiation field. The influence of the molar ratio of glutathione to titanium oxide and the effect of the fold of NaOH vs. stoichiometric amount on the size of the formed TiO2 nanoparticles was determined. The physicochemical properties of the obtained products were evaluated using dynamic light scattering (DLS), transmission electron microscope- energy-dispersive X-ray spectroscopy (TEM-EDS), low-temperature nitrogen adsorption method (BET), X-Ray Diffraction (XRD) and Fourier-transform infrared spectroscopy (FTIR) microscopy methods. The size of TiO2 nanoparticles was characterized from 30 nm to 336 nm. The release of titanium ions from the prepared products was evaluated. These studies were carried out using different media in which the powders were incubated for a specific time. These were: water, SBF and Ringer's solution. The release of titanium ions from modified products is weaker compared to unmodified titanium oxide nanoparticles. The reduced release of titanium ions may allow the use of such modified materials as substances in drug delivery systems.
Abstract: Drug delivery to target human acute myeloid leukemia (AML) using a nanoparticulate chemotherapeutic formulation that can deliver drugs selectively to AML cancer is hugely needed. In this work, we report the development of a nanoformulation made of polymeric-stabilized multifunctional magnetic iron oxide nanoparticles (PMNP) loaded with the anticancer drug Doxorubicin (Dox) as a promising drug carrier to treat AML. Dox@PMNP conjugates simultaneously exhibited high drug content, maximized fluorescence, and excellent release properties. Nanoparticulate uptake and cell death following addition of Dox@PMNPs were then evaluated in different types of human AML target cells, as well as on normal human cells. While the unloaded MNPs were not toxic to any of the cells, Dox@PMNPs were found to be highly toxic to the different AML cell lines, albeit at different inhibitory concentrations (IC50 values), but showed very little toxicity towards the normal cells. In comparison, free Dox showed significant potency concurrently to all the cell lines, suggesting huge potentials for the use of Dox@PMNPs as selective AML anticancer cargos. Live confocal imaging, fluorescence and electron microscopy confirmed that Dox is indeed delivered to the nucleus in relatively short periods of time, causing apoptotic cell death. Importantly, this targeted payload may potentially enhance the effectiveness of the drug in AML patients and may further allow physicians to image leukemic cells exposed to Dox@PMNPs using MRI.
Abstract: Application of nanoscience in biomedical field has come across as a new era. This study involves the synthesis of nano drug carrier with antibiotic loading. Based on the founding that polydopamine (PDA) nanoparticles could be formed via self-polymerization of dopamine at alkaline pH, one-step synthesis of rifampicin coupled polydopamine (PDA-R) nanoparticles was achieved by adding rifampicin into the dopamine solution. The successful yield of PDA nanoparticles with or without the presence of rifampicin during the polymerization process was characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, and Raman spectroscopy. Drug loading was monitored by UV-vis spectroscopy and the loading efficiency of rifampicin was calculated to be 76%. Such highly capacious nano-reservoir was found very stable with little drug leakage at pH 3.
Abstract: Liposome plays an important role in medical and
pharmaceutical science as e.g. nano scale drug carriers. Liposomes
are vesicles of varying size consisting of a spherical lipid bilayer and
an aqueous inner compartment. Magnet-driven liposome used for the
targeted delivery of drugs to organs and tissues. These liposome
preparations contain encapsulated drug components and finely
dispersed magnetic particles.
Liposomes are vesicles of varying size consisting of a spherical
lipid bilayer and an aqueous inner compartment that are generated in
vitro. These are useful in terms of biocompatibility, biodegradability,
and low toxicity, and can control biodistribution by changing the size,
lipid composition, and physical characteristics. Furthermore,
liposomes can entrap both hydrophobic and hydrophilic drugs and are
able to continuously release the entrapped substrate, thus being useful
drug carriers. Magnetic liposomes (MLs) are phospholipid vesicles
that encapsulate magneticor paramagnetic nanoparticles. They are
applied as contrast agents for magnetic resonance imaging (MRI).
The biological synthesis of nanoparticles using plant extracts plays
an important role in the field of nanotechnology. Green-synthesized
magnetite nanoparticles-protein hybrid has been produced by treating
Iron (III) / Iron (II) chloride with the leaf extract of Datura inoxia.
The phytochemicals present in the leaf extracts act as a reducing as
well stabilizing agents preventing agglomeration, which include
flavonoids, phenolic compounds, cardiac glycosides, proteins and
sugars.
The magnetite nanoparticles-protein hybrid has been trapped
inside the aqueous core of the liposome prepared by reversed phase
evaporation (REV) method using oleic and linoleic acid which has
been shown to be driven under magnetic field confirming the
formation magnetic liposome (ML). Chemical characterization of
stealth magnetic liposome has been performed by breaking the
liposome and release of magnetic nanoparticles. The presence iron
has been confirmed by colour complex formation with KSCN and
UV-Vis study using spectrophotometer Cary 60, Agilent.
This magnet driven liposome using nanoparticles-protein hybrid
can be a smart vesicles for the targeted drug delivery.
Abstract: Most of the drugs used for pharmaceutical purposes
are poorly water-soluble drugs. About 40% of all newly discovered
drugs are lipophilic and the numbers of lipophilic drugs seem to
increase more and more. Drug delivery systems such as
nanoparticles, micelles or liposomes are applied to improve their
solubility and thus their bioavailability. Besides various techniques of
solubilization, oil-in-water emulsions are often used to incorporate
lipophilic drugs into the oil phase. To stabilize emulsions surface
active substances (surfactants) are generally used. An alternative
method to avoid the application of surfactants was of great interest.
One possibility is to develop O/W-emulsion without any addition of
surface active agents or the so called “surfactant-free emulsion or
SFE”. The aim of this study was to develop and characterize SFE as a
drug carrier by varying the production conditions. Lidocaine base
was used as a model drug. The injection method was developed.
Effects of ultrasound as well as of temperature on the properties of
the emulsion were studied. Particle sizes and release were
determined. The long-term stability up to 30 days was performed.
The results showed that the surfactant-free O/W emulsions with
pharmaceutical oil as drug carrier can be produced.
Abstract: The expression of LFA-1 diverges from the
physiological condition, thus active targeting carrier can provide the
benefits from difference into LFA-1 expression in various conditions.
Here, the selectivity of cIBR-conjugated nanoparticles (cIBR-NPs),
in terms of uptake, was investigated using PBMCs, Mixed PBMCMolt-
3 cells and Molt-3 cells. The expressions of LFA-1 on Molt-3
cells, from flow cytometry and Western blot, possessed the highest
level whereas PBMCs showed the lowest level. The kinetic uptake
profiles of cIBR-NPs were obtained by flow cytometry, which the
degree of cellular uptake presented a similar trend with the level of
LFA-1 indicating the influence of LFA-1 expression on the cellular
uptake of cIBR-NPs. The conformation of LFA-1 had a slight effect
on the cellular uptake of cIBR-NPs. Overall we demonstrated that
cIBR-NPs enhanced cellular uptake and improved the selectivity of
drug carriers to LFA-1 on the leukemia cells, which related with the
order of LFA-1 expression.