Abstract: A statistical optimization of the saccharification
process of EFB was studied. The statistical analysis was done by
applying faced centered central composite design (FCCCD) under
response surface methodology (RSM). In this investigation, EFB
dose, enzyme dose and saccharification period was examined, and the
maximum 53.45% (w/w) yield of reducing sugar was found with 4%
(w/v) of EFB, 10% (v/v) of enzyme after 120 hours of incubation. It
can be calculated that the conversion rate of cellulose content of the
substrate is more than 75% (w/w) which can be considered as a
remarkable achievement. All the variables, linear, quadratic and
interaction coefficient, were found to be highly significant, other than
two coefficients, one quadratic and another interaction coefficient.
The coefficient of determination (R2) is 0.9898 that confirms a
satisfactory data and indicated that approximately 98.98% of the
variability in the dependent variable, saccharification of EFB, could
be explained by this model.
Abstract: A statistical optimization was studied to design a media composition to produce optimum cellulolytic enzyme where palm oil mill effluent (POME) as a basal medium and filamentous fungus, Trichoderma reesei RUT-C30 were used in the liquid state bioconversion(LSB). 2% (w/v) total suspended solid, TSS, of the POME supplemented with 1% (w/v) cellulose, 0.5%(w/v) peptone and 0.02% (v/v) Tween 80 was estimated to produce the optimum CMCase activity of 18.53 U/ml through the statistical analysis followed by the faced centered central composite design(FCCCD). The probability values of cellulose (