The Construction of a Probiotic Lactic Acid Bacterium Expressing Acid-Resistant Phytase Enzyme

The use of probiotics engineered to express specific enzymes has been the subject of considerable attention in poultry industry because of increased nutrient availability and reduced cost of enzyme supplementation. Phytase enzyme is commonly added to poultry feed to improve digestibility and availability of phosphorus from plant sources. To construct a probiotic with potential of phytate degradation, phytase gene (appA) from E. coli was cloned and transformed into two probiotic bacteria Lactobacillus salivarius and Lactococcus lactis. L. salivarous showed plasmid instability, unable to express the gene. The expression of appA gene in L. lactis was analyzed by detecting specific RNA and zymography assay. Phytase enzyme was isolated from cellular extracts of recombinant L. lactis, showing a 46 kDa band upon the SDS-PAGE analysis. Zymogram also confirmed the phytase activity of the 46 kDa band corresponding to the enzyme. An enzyme activity of 4.9U/ml was obtained in cell extracts of L. lactis. The growth of native and recombinant L. lactis was similar in the presence of two concentrations of ox bile.

Identifying Karst Pattern to Prevent Bell Spring from Being Submerged in Daryan Dam Reservoir

The large karstic Bell spring with a discharge ranging between 250 and 5300 lit/ sec is one of the most important springs of Kermanshah Province. This spring supplies drinking water of Nodsheh City and its surrounding villages. The spring is located in the reservoir of Daryan Dam and its mouth would be submerged after impounding under a water column of about 110 m height. This paper has aimed to render an account of the karstification pattern around the spring under consideration with the intention of preventing Bell Spring from being submerged in Daryan Dam Reservoir. The studies comprise engineering geology and hydrogeology investigations. Some geotechnical activities included in these studies include geophysical studies, drilling, excavation of exploratory gallery and shaft and diving. The results depict that Bell is a single-conduit siphon spring with 4 m diameter and 85 m height that 32 m of the conduit is located below the spring outlet. To survive the spring, it was decided to plug the outlet and convey the water to upper elevations under the natural pressure of the aquifer. After plugging, water was successfully conveyed to elevation 837 meter above sea level (about 120 m from the outlet) under the natural pressure of the aquifer. This signifies the accuracy of the studies done and proper recognition of the karstification pattern of Bell Spring. This is a unique experience in karst problems in Iran.

Effect of Transplant Preparation Method on Yield and Agronomic Traits of True Potato Seed (TPS) Progenies in Sahneh Region

To study the effect of suitable methods for propagation of True Potato Seed (TPS) progenies, transplant and selection of the best progenies, a factorial experiment base on a randomized complete block design was carried out in the research field of Sahneh region, Kermanshah, Iran during 2009-2010. Five selective progenies from CIP (International Potato Center) including CIP.994013, CIP.994002, CIP.994014, CIP.888006, and CIP.994001 and two transplant preparation methods (Paper pot preparation for mechanical cultivation and preparation in transplant trays for manual cultivation) were studied in three replications. Results showed that different progenies had no significant effect on plant height (cm) and tuber yield (t ha-1), whereas had a significant effect on number of tubers per unit area (m2). There was significant difference between transplant preparation methods for plant height and tuber yield. The interaction effect of progenies and transplant preparation method was not significant for these traits. CIP.888006 progeny and paper pot preparation method produced the highest tuber yields. Also CIP.994002 and CIP.994014 progenies considered as the best progenies under paper pot preparation method due to high yields.

Evaluating the Standards of Hospital Pharmacies in Therapeutic Centers Affiliated with Kermanshah University of Medical Sciences, Iran

Nowadays pharmaceutical care departments located in hospitals are amongst the important pillars of the healthcare system. The aim of this study was to evaluate quality of hospital drugstores affiliated with Kermanshah University of Medical Sciences. In this cross-sectional study a validated questionnaire was used. The questionnaire was filled in by the one of the researchers in all seventeen hospital drugstores located in the teaching and nonteaching hospitals affiliated with Kermanshah University of Medical Sciences. The results shows that in observed hospitals,24% of pharmacy environments, 25% of pharmacy store and storage conditions, 49% of storage procedure, 25% of ordering drugs and supplies, 73% of receiving supplies (proper procedure are fallowed for receiving supplies), 35% of receiving supplies (prompt action taken if deterioration of drugs received is suspected), 23.35% of drugs delivery to patients and finally 0% of stock cards are used for proper inventory control have full compliance with standards.

Effect of the Internet on Social Capital

Internet access is a vital part of the modern world and an important tool in the education of our children. It is present in schools, homes and even shopping malls. Mastering the use of the internet is likely to be an important skill for those entering the job markets of the future. An internet user can be anyone he or she wants to be in an online chat room, or play thrilling and challenging games against other players from all corners of the globe. It seems at present time (or near future) for many people relationships in the real world may be neglected as those in the virtual world increase in importance. Internet is provided a fast mode of transportation caused freedom from family bonds and mixing with different cultures and new communities. This research is an attempt to study effect of Internet on Social capital. For this purpose a survey technique on the sample size amounted 168 students of Payame Noor University of Kermanshah city in country of Iran were considered. Degree of social capital is moderate. With the help of the Multi-variable Regression, variables of Iranian message attractive, Interest to internet with effect of positive and variable Creating a cordial atmosphere with negative effect be significant.

The Response of Winter Wheat to Flooding

The effect of flooding can be a serious problem for wheat farmers, even at dry land condition. Amount of flooding damage depends on duration flooding, developmental stage, wheat type and variety. Therefore as a factorial experiment in randomized complete design based on winter bread wheat cultivars (Pishtaz, Marvdasht, Shiraz, Zarin, Shahriar, C-81-4, Sardari, Agosta seed, FGS and Azar2) at stages (Non- flooding stress, flooding at tillering and stem elongation stages for 15 days) carried out in Faculty of Agriculture, Razi University, Kermanshah, Iran. During flooding, soil environment of plant roots were water saturated. Analysis of variance showed that flooding had a significant effect on the number of grains per spike, grain weight per spike and a grain weight. Hence flooding reduces the number of grain per spike between 27.1 to 42.5 percent, grain weight per spike between 34.7 to 54.4 percent and single grain weight between 12.1 to 15.1 percent. Effects of flooding at the tillering stage reduced higher than stem elongation stage on studied traits. The result also showed that flooding at tillering stage delayed spikelet primordial and floret. Between wheat cultivars was significant for traits, but were different reactions. "Shiraz", "Zarin" and "Shahriar" had the most no. grain per spike, but "Zarin" and "Sardari" had the most grain weight per spike and single grain weight, respectively. Also, interaction between start of flooding and cultivar was significant.

A Green Chemical Technique for the Synthesis of Magnetic Nanoparticles by Magnetotactic Bacteria

Bacterial magnetic nanoparticles have great useful potential in biotechnological and biomedical applications. In this study, a liquid growth medium was modified for cultivation a fastidious magnetotactic bacterium that has been isolated from Anzali lagoon, Iran in our previous research. These modifications include change in vitamin, mineral, carbon sources and etcetera. In our experience, the serum bottles and designed air-tight laboratory bottles were used to create microaerobic conditions in order to development of a method for scale-up experiment. This information may serve as a guide to green chemistry based biological protocols for the synthesis of magnetic nanoparticles with control over the chemical composition, morphology and size.

Application of Artificial Neural Networks for Temperature Forecasting

In this paper, the application of neural networks to study the design of short-term temperature forecasting (STTF) Systems for Kermanshah city, west of Iran was explored. One important architecture of neural networks named Multi-Layer Perceptron (MLP) to model STTF systems is used. Our study based on MLP was trained and tested using ten years (1996-2006) meteorological data. The results show that MLP network has the minimum forecasting error and can be considered as a good method to model the STTF systems.

Evaluating the Response of Rainfed-Chickpea to Population Density in Iran, Using Simulation

The response of growth and yield of rainfed-chickpea to population density should be evaluated based on long-term experiments to include the climate variability. This is achievable just by simulation. In this simulation study, this evaluation was done by running the CYRUS model for long-term daily weather data of five locations in Iran. The tested population densities were 7 to 59 (with interval of 2) stands per square meter. Various functions, including quadratic, segmented, beta, broken linear, and dent-like functions, were tested. Considering root mean square of deviations and linear regression statistics [intercept (a), slope (b), and correlation coefficient (r)] for predicted versus observed variables, the quadratic and broken linear functions appeared to be appropriate for describing the changes in biomass and grain yield, and in harvest index, respectively. Results indicated that in all locations, grain yield tends to show increasing trend with crowding the population, but subsequently decreases. This was also true for biomass in five locations. The harvest index appeared to have plateau state across low population densities, but decreasing trend with more increasing density. The turning point (optimum population density) for grain yield was 30.68 stands per square meter in Isfahan, 30.54 in Shiraz, 31.47 in Kermanshah, 34.85 in Tabriz, and 32.00 in Mashhad. The optimum population density for biomass ranged from 24.6 (in Tabriz) to 35.3 stands per square meter (Mashhad). For harvest index it varied between 35.87 and 40.12 stands per square meter.

Effects of PEG and NaCl Stress on Two Cultivars of Corn (Zea mays L.) at Germination and Early Seedling Stages

To study on effect of PEG and NaCl stress on germination and early seedling stages on two cultivar of corn, two separated experiment were laid out at physiology laboratory, faculty of Agriculture, Razi University, Kermanshah, Iran in 2009. This investigation was performed as factorial experiment under Complete Randomized Design (CRD) with three replications. Cultivar factor contains of two varieties (sweet corn SC403 and Flint corn SC704) and five levels of stress (0, -2, -4, -6 and -8 bar). The principal aim of current study was to compare the two varieties of maize in relative to the stress conditions. Results indicated that significant decrease was observed in percentage of germination, germination rate, length of radicle and plumule and radicle and plumule dry matter. On the basis of the results, NaCl as compared with PEG had more effect on germination and early seedling stage and sweet corn had more resistant than flint corn in both stress conditions.

Effects of Late Sowing on Quality of Coriander (Coriandrum sativum L.)

Coriander is an annual and herbaceous plant, belong to the apiaceae family. This plant is cultivated world widely. It is well known for having medicinal properties. The aim of this experiment was to study seed quality of species grown in Kermanshah conditions. The experiment was carried out in research farm, Campus of Agriculture and Natural Resources, Razi University, Kermanshah, Iran. Coriander (local type) was grown in late spring May (5th and 20th) and Jun (4th and 19th), and plant density (10, 30, 50 and 70 plants m-2) in 2009. The experimental plots were laid out in a factorial according to a randomized complete block design with three replications. The fruits were harvest between 83.5 – 106.5 days after sowing. The essential oil and oil content was extracted by Clevenger and Soxhlet apparatuses, respectively. Results showed that delay at planting date increased the oil content. Also, with the increase at plant density was decreased oil content and essential oil.

Synthesis of Sterile and Pyrogen Free Biogenic Magnetic Nanoparticles: Biotechnological Potential of Magnetotactic Bacteria for Production of Nanomaterials

Today, biogenic magnetite nanoparticles among magnetic nanoparticles have unique attracted attention because of their magnetic characteristics and potential applications in various fields such as therapeutic and diagnostic. A well known example of these biogenic nanoparticles is magnetosomes of magnetotactic bacteria. In this research, we used two different types of technique for the isolation and purification of magnetosome nanoparticles from the isolated magnetotactic bacterial cells, heat-alkaline treatment and sonication. Also we evaluated pyrogen content and sterility of synthesized the isolated individual magnetosome by the Limulus Amoebocyte Lysate test and direct impedimetric method respectively.

A Materialized Approach to the Integration of XML Documents: the OSIX System

The data exchanged on the Web are of different nature from those treated by the classical database management systems; these data are called semi-structured data since they do not have a regular and static structure like data found in a relational database; their schema is dynamic and may contain missing data or types. Therefore, the needs for developing further techniques and algorithms to exploit and integrate such data, and extract relevant information for the user have been raised. In this paper we present the system OSIX (Osiris based System for Integration of XML Sources). This system has a Data Warehouse model designed for the integration of semi-structured data and more precisely for the integration of XML documents. The architecture of OSIX relies on the Osiris system, a DL-based model designed for the representation and management of databases and knowledge bases. Osiris is a viewbased data model whose indexing system supports semantic query optimization. We show that the problem of query processing on a XML source is optimized by the indexing approach proposed by Osiris.