Abstract: The present study is a foundational study for performance improvements on isolation wards to prevent proliferation of secondary infection of infectious diseases such as SARS, H1N1, and MERS inside hospitals. Accordingly, the present study conducted an analysis of the effect of sealing mechanisms and filling of openings on ensuring air tightness performance in isolation wards as well as simulation on air currents in improved isolation wards. The study method is as follows. First, previous studies on aerial infection type and mechanism were reviewed, and the review results were utilized as basic data of analysis on simulation of air current. Second, national and international legislations and regulations in relation to isolation wards as well as case studies on developed nations were investigated in order to identify the problems in isolation wards in Korea and improvement plans. Third, construction and facility plans were compared and analyzed between general and isolation wards focusing on large general hospitals in Korea, thereby conducting comparison and analysis on the performance and effects of air-tightness of general and isolation wards through CFD simulations. The study results showed that isolation wards had better air-tightness performance than that of general wards.
Abstract: Viral influenza A subtypes H5N1 and pandemic
H1N1 (pH1N1) have worldwide emerged and transmitted. The most
common anti-influenza drug for treatment of both seasonal and
pandemic influenza viruses is oseltamivir that nowadays becomes
resistance to influenza neuraminidase. The novel long-acting drug,
laninamivir, was discovered for treatment of the patients infected
with influenza B and influenza A viruses. In the present study,
laninamivir complexed with wild-type strain of both H5N1 and
pH1N1 viruses were comparatively determined the structures and
drug-target interactions by means of molecular dynamics (MD)
simulations. The results show that the hydrogen bonding interactions
formed between laninamivir and its binding residues are likely
similar for the two systems. Additionally, the presence of
intermolecular interactions from laninamivir to the residues in the
binding pocket is established through their side chains in accordance
with hydrogen bond interactions.
Abstract: Weblogs are resource of social structure to discover and track the various type of information written by blogger. In this paper, we proposed to use mining weblogs technique for identifying the trends of influenza where blogger had disseminated their opinion for the anomaly disease. In order to identify the trends, web crawler is applied to perform a search and generated a list of visited links based on a set of influenza keywords. This information is used to implement the analytics report system for monitoring and analyzing the pattern and trends of influenza (H1N1). Statistical and graphical analysis reports are generated. Both types of the report have shown satisfactory reports that reflect the awareness of Malaysian on the issue of influenza outbreak through blogs.
Abstract: In this study, three subtypes of influenza A viruses (pH1N1, H5N1 and H3N2) which naturally infected human were analyzed by bioinformatic approaches to find candidate human cellular miRNAs targeting viral genomes. There were 76 miRNAs targeting influenza A viruses. Among these candidates, 70 miRNAs were subtypes specifically targeting each subtype of influenza A virus including 21 miRNAs targeted subtype H1N1, 27 miRNAs targeted subtype H5N1 and 22 miRNAs targeted subtype H3N2. The remaining 6 miRNAs target on multiple subtypes of influenza A viruses. Uniquely, hsa-miR-3145 is the only one candidate miRNA targeting PB1 gene of all three subtypes. Obviously, most of the candidate miRNAs are targeting on polymerase complex genes (PB2, PB1 and PA) of influenza A viruses. This study predicted potential human miRNAs targeting on different subtypes of influenza A viruses which might be useful for inhibition of viral replication and for better understanding of the interaction between virus and host cell.
Abstract: The aims of this paper are to study the efficacy of
chitosan nanoparticles in stimulating specific antibody against
A/H1N1 influenza antigen in mice. Chitosan nanoparticles (CSN)
were characterized by TEM. The results showed that the average size
of CSN was from 80nm to 106nm. The efficacy of A/H1N1 influenza
vaccine loaded on the surface of CSN showed that loading efficiency
of A/H1N1 influenza antigen on CSN was from 93.75 to 100%. Safe
property of the vaccine were tested. In 10 days post vaccination,
group of CSN 30 kDa and 300 kDa loaded A/H1N1 influenza antigen
were the rate of immune response on mice to be 100% (9/9) higher
than Al(OH)3 and other adjuvant. 100% mice in the experiment of all
groups had immune response in 20 days post vaccination. The results
also showed that HI titer of the group using CSN 300 kDa as an
adjuvant increased significantly up to 3971 HIU, over three-fold
higher than the Al(OH)3 adjuvant, chitosan (CS), and one hundredfold
than the A/H1N1 antigen only. Stability of the vaccine
formulation was investigated.
Abstract: The Swine flu outbreak in humans is due to a new
strain of influenza A virus subtype H1N1 that derives in part from
human influenza, avian influenza, and two separated strains of swine
influenza. It can be transmitted from human to human. A
mathematical model for the transmission of Swine flu is developed in
which the human populations are divided into two classes, the risk
and non-risk human classes. Each class is separated into susceptible,
exposed, infectious, quarantine and recovered sub-classes. In this
paper, we formulate the dynamical model of Swine flu transmission
and the repetitive contacts between the people are also considered.
We analyze the behavior for the transmission of this disease. The
Threshold condition of this disease is found and numerical results are
shown to confirm our theoretical predictions.
Abstract: In April 2009, a new variant of Influenza A virus
subtype H1N1 emerged in Mexico and spread all over the world. The
influenza has three subtypes in human (H1N1, H1N2 and H3N2)
Types B and C influenza tend to be associated with local or regional
epidemics. Preliminary genetic characterization of the influenza
viruses has identified them as swine influenza A (H1N1) viruses.
Nucleotide sequence analysis of the Haemagglutinin (HA) and
Neuraminidase (NA) are similar to each other and the majority of
their genes of swine influenza viruses, two genes coding for the
neuraminidase (NA) and matrix (M) proteins are similar to
corresponding genes of swine influenza. Sequence similarity between
the 2009 A (H1N1) virus and its nearest relatives indicates that its
gene segments have been circulating undetected for an extended
period. Nucleic acid sequence Maximum Likelihood (MCL) and
DNA Empirical base frequencies, Phylogenetic relationship amongst
the HA genes of H1N1 virus isolated in Genbank having high
nucleotide sequence homology.
In this paper we used 16 HA nucleotide sequences from NCBI for
computing sequence relationships similarity of swine influenza A
virus using the following method MCL the result is 28%, 36.64% for
Optimal tree with the sum of branch length, 35.62% for Interior
branch phylogeny Neighber – Join Tree, 1.85% for the overall
transition/transversion, and 8.28% for Overall mean distance.
Abstract: In recent years, several severe large-scale influenza
outbreaks happened in many countries, such as SARS in 2005 or
H1N1 in 2009. Those influenza Epidemics have greatly impacts not
only on people-s life and health, but medical systems in different
countries. Although severe diseases are more experienced, they are not
fully controlled. Governments have different policies to control the
spreads of diseases. However, those policies have both positive and
negative social or economical influence on people and society.
Therefore, it is necessary and essential to develop an appropriate
model for evaluations of policies. Consequently, a proper measure can
be implemented to confront the diseases. The main goal of this study is
to develop a SIR-based model for the further evaluations of the
candidate policies during the influenza outbreaks.
Abstract: A new strain of Type A influenza virus can cause the
transmission of H1N1 virus. This virus can spread between the
people by coughing and sneezing. Because the people are always
movement, so this virus can be easily spread. In this study, we
construct the dynamical network model of H1N1 virus by separating
the human into five groups; susceptible, exposed, infectious,
quarantine and recovered groups. The movement of people between
houses (local level) is considered. The behaviors of solutions to our
dynamical model are shown for the different parameters.