Abstract: This paper presents an analysis of the localization accuracy of indoor positioning systems using Cramer-s rule via IEEE 802.15.4 wireless sensor networks. The objective is to study the impact of the methods used to convert the received signal strength into the distance that is used to compute the object location in the wireless indoor positioning system. Various methods were tested and the localization accuracy was analyzed. The experimental results show that the method based on the empirical data measured in the non line-of-sight (NLOS) environment yield the highest localization accuracy; with the minimum error distance less than 3 m.