Abstract: The counting process of cell colonies is always a long
and laborious process that is dependent on the judgment and ability
of the operator. The judgment of the operator in counting can vary in
relation to fatigue. Moreover, since this activity is time consuming it
can limit the usable number of dishes for each experiment. For these
purposes, it is necessary that an automatic system of cell colony
counting is used. This article introduces a new automatic system of
counting based on the elaboration of the digital images of cellular
colonies grown on petri dishes. This system is mainly based on the
algorithms of region-growing for the recognition of the regions of
interest (ROI) in the image and a Sanger neural net for the
characterization of such regions. The better final classification is
supplied from a Feed-Forward Neural Net (FF-NN) and confronted
with the K-Nearest Neighbour (K-NN) and a Linear Discriminative
Function (LDF). The preliminary results are shown.
Abstract: Semiconductor nanomaterials like TiO2 nanoparticles
(TiO2-NPs) approximately less than 100 nm in diameter have become
a new generation of advanced materials due to their novel and
interesting optical, dielectric, and photo-catalytic properties. With the
increasing use of NPs in commerce, to date few studies have
investigated the toxicological and environmental effects of NPs.
Motivated by the importance of TiO2-NPs that may contribute to the
cancer research field especially from the treatment prospective
together with the fractal analysis technique, we have investigated the
effect of TiO2-NPs on colony morphology in the dark condition
using fractal dimension as a key morphological characterization
parameter. The aim of this work is mainly to investigate the cytotoxic
effects of TiO2-NPs in the dark on the growth of human cervical
carcinoma (HeLa) cell colonies from morphological aspect. The in
vitro studies were carried out together with the image processing
technique and fractal analysis. It was found that, these colonies were
abnormal in shape and size. Moreover, the size of the control
colonies appeared to be larger than those of the treated group. The
mean Df +/- SEM of the colonies in untreated cultures was
1.085±0.019, N= 25, while that of the cultures treated with TiO2-NPs
was 1.287±0.045. It was found that the circularity of the control
group (0.401±0.071) is higher than that of the treated group
(0.103±0.042). The same tendency was found in the diameter
parameters which are 1161.30±219.56 μm and 852.28±206.50 μm
for the control and treated group respectively. Possible explanation of
the results was discussed, though more works need to be done in
terms of the for mechanism aspects. Finally, our results indicate that
fractal dimension can serve as a useful feature, by itself or in
conjunction with other shape features, in the classification of cancer
colonies.