Abstract: Quantitative radiobiological models can be used to
assess the optimum clinical outcome from sophisticated therapeutic
modalities by calculating tumor control probability (TCP) and normal
tissue complication probability (NTCP). In this study two 3D-CRT
and an IMRT treatment plans were developed with an initial
prescription dose of 60 Gy in 2 Gy/fraction to prostate. Sensitivity of
TCP and Complication free tumor control probability (P+) to the
different values of α/β ratio was investigated for various prescription
doses planned to be delivered in either a fixed number of fractions (I)
or in a fixed dose per fraction (II) in each of the three different
treatment plans. High dose/fraction and high α/β value result in
comparatively smaller P+ and IMRT plans resulted in the highest P+,
mainly due to the decrease in NTCP. If α/β is lower than expected,
better tumor control can be achieved by increasing dose/fraction but
decreasing the number of fractions.
Abstract: Background: The objectives of this study were to
assess patient’s knowledge of appropriate sublingual glyceryl
trinitrate (GTN) use as well as to investigate how patients commonly
store and carry their sublingual GTN tablets. Methodology: This was
a cross-sectional survey, using a validated researcher-administered
questionnaire. The study involved cardiac patients receiving
sublingual GTN attending the outpatient and inpatient departments of
Taiping Hospital, a non-academic public care hospital. The minimum
calculated sample size was 92, but 100 patients were conveniently
sampled. Respondents were interviewed on 3 areas, including
demographic data, knowledge and use of sublingual GTN. Eight
items were used to calculate each subject’s knowledge score and six
items were used to calculate use score. Results: Of the 96 patients
who consented to participate, majority (96.9%) were well aware of
the indication of sublingual GTN. With regards to the mechanism of
action of sublingual GTN, 73 (76%) patients did not know how the
medication works. Majority of the patients (66.7%) knew about the
proper storage of the tablet. In relation to the maximum number of
sublingual GTN tablets that can be taken during each angina episode,
36.5% did not know that up to 3 tablets of sublingual GTN can be
taken during each episode of angina. Fifty four (56.2%) patients were
not aware that they need to replace sublingual GTN every 8 weeks
after receiving the tablets. Majority (69.8%) of the patients
demonstrated lack of knowledge with regards to the use of sublingual
GTN as prevention of chest pain. Conclusion: Overall, patients’
knowledge regarding the self-administration of sublingual GTN is
still inadequate. The findings support the need for more frequent
reinforcement of patient education, especially in the areas of
preventive use, storage and drug stability.
Abstract: Microarray technology is universally used in the study
of disease diagnosis using gene expression levels. The main
shortcoming of gene expression data is that it includes thousands of
genes and a small number of samples. Abundant methods and
techniques have been proposed for tumor classification using
microarray gene expression data. Feature or gene selection methods
can be used to mine the genes that directly involve in the
classification and to eliminate irrelevant genes. In this paper
statistical measures like T-Statistics, Signal-to-Noise Ratio (SNR)
and F-Statistics are used to rank the genes. The ranked genes are used
for further classification. Particle Swarm Optimization (PSO)
algorithm and Shuffled Frog Leaping (SFL) algorithm are used to
find the significant genes from the top-m ranked genes. The Naïve
Bayes Classifier (NBC) is used to classify the samples based on the
significant genes. The proposed work is applied on Lung and Ovarian
datasets. The experimental results show that the proposed method
achieves 100% accuracy in all the three datasets and the results are
compared with previous works.
Abstract: The article demonstrates on a case study how it is
possible to identify MSD risk. It is based on a dissertation Risk
identification model of occupational diseases formation in relation to
the work activity that determines what risk can endanger workers who
are exposed to the specific risk factors. It is evaluated based on
statistical calculations. These risk factors are main cause of upperextremities
musculoskeletal disorders.
Abstract: A method which allows a diabetic quadriplegic patient
that has had four limb amputations (above the knee and elbow) to
self-administer injections of insulin has been designed. The aim of
this research project is to improve a quadriplegic patient’s selfmanagement,
affected by diabetes, by designing a suitable device for
self-administering insulin.
The quadriplegic patient affected by diabetes has to be able to selfadminister
insulin safely and independently to guarantee stable
healthy conditions. The device also should be designed to adapt to a
number of different varying personal characteristics such as height
and body weight.
Abstract: Kinematic data wisely correlate vector quantities in
space to scalar parameters in time to assess the degree of symmetry
between the intact limb and the amputated limb with respect to a
normal model derived from the gait of control group participants.
Furthermore, these particular data allow a doctor to preliminarily
evaluate the usefulness of a certain rehabilitation therapy.
Kinetic curves allow the analysis of ground reaction forces (GRFs)
to assess the appropriateness of human motion.
Electromyography (EMG) allows the analysis of the fundamental
lower limb force contributions to quantify the level of gait
asymmetry. However, the use of this technological tool is expensive
and requires patient’s hospitalization. This research work suggests
overcoming the above limitations by applying artificial neural
networks.
Abstract: In this research work, neural networks were applied to
classify two types of hip joint implants based on the relative hip joint
implant side speed and three components of each ground reaction
force. The condition of walking gait at normal velocity was used and
carried out with each of the two hip joint implants assessed. Ground
reaction forces’ kinetic temporal changes were considered in the first
approach followed but discarded in the second one. Ground reaction
force components were obtained from eighteen patients under such
gait condition, half of which had a hip implant type I-II, whilst the
other half had the hip implant, defined as type III by Orthoload®.
After pre-processing raw gait kinetic data and selecting the time
frames needed for the analysis, the ground reaction force components
were used to train a MLP neural network, which learnt to distinguish
the two hip joint implants in the abovementioned condition. Further
to training, unknown hip implant side and ground reaction force
components were presented to the neural networks, which assigned
those features into the right class with a reasonably high accuracy for
the hip implant type I-II and the type III. The results suggest that
neural networks could be successfully applied in the performance
assessment of hip joint implants.
Abstract: In healthy humans, the cortical brain rhythm shows
specific mu (~6-14 Hz) and beta (~18-24 Hz) band patterns in the
cases of both real and imaginary motor movements. As cerebellar
ataxia is associated with impairment of precise motor movement
control as well as motor imagery, ataxia is an ideal model system in
which to study the role of the cerebellocortical circuit in rhythm
control. We hypothesize that the EEG characteristics of ataxic patients
differ from those of controls during the performance of a
Brain-Computer Interface (BCI) task. Ataxia and control subjects
showed a similar distribution of mu power during cued relaxation.
During cued motor imagery, however, the ataxia group showed
significant spatial distribution of the response, while the control group
showed the expected decrease in mu-band power (localized to the
motor cortex).
Abstract: A blood pressure monitor or sphygmomanometer can
be either manual or automatic, employing respectively either the
auscultatory method or the oscillometric method.
The manual version of the sphygmomanometer involves an
inflatable cuff with a stethoscope adopted to detect the sounds
generated by the arterial walls to measure blood pressure in an artery.
An automatic sphygmomanometer can be effectively used to
monitor blood pressure through a pressure sensor, which detects
vibrations provoked by oscillations of the arterial walls.
The pressure sensor implemented in this device improves the
accuracy of the measurements taken.
Abstract: Second line antiretroviral therapy (ART) regimen is
used when patients fail their first line regimen. There are many
factors such as non-adherence, drug resistance as well as virological
and immunological failure that lead to second line highly active
antiretroviral therapy (HAART) regimen treatment failure. This study
was aimed at determining predictor factors to treatment failure with
second line HAART and analyzing median survival time.
An observational, retrospective study was conducted in Sungai
Buloh Hospital (HSB) to assess current status of HIV patients treated
with second line HAART regimen. Convenience sampling was used
and 104 patients were included based on the study’s inclusion and
exclusion criteria. Data was collected for six months i.e. from July
until December 2013. Data was then analysed using SPSS version 18.
Kaplan-Meier and Cox regression analyses were used to measure
median survival times and predictor factors for treatment failure.
The study population consisted mainly of male subjects, aged 30-
45 years, who were heterosexual, and had HIV infection for less than
6 years. The most common second line HAART regimen given was
lopinavir/ritonavir (LPV/r)-based combination. Kaplan-Meier
analysis showed that patients on LPV/r demonstrated longer median
survival times than patients on indinavir/ritonavir (IDV/r) based
combination (p
Abstract: Red blood cells (RBCs) are among the most
commonly and intensively studied type of blood cells in cell biology.
Anemia is a lack of RBCs is characterized by its level compared to
the normal hemoglobin level. In this study, a system based image
processing methodology was developed to localize and extract RBCs
from microscopic images. Also, the machine learning approach is
adopted to classify the localized anemic RBCs images. Several
textural and geometrical features are calculated for each extracted
RBCs. The training set of features was analyzed using principal
component analysis (PCA). With the proposed method, RBCs were
isolated in 4.3secondsfrom an image containing 18 to 27 cells. The
reasons behind using PCA are its low computation complexity and
suitability to find the most discriminating features which can lead to
accurate classification decisions. Our classifier algorithm yielded
accuracy rates of 100%, 99.99%, and 96.50% for K-nearest neighbor
(K-NN) algorithm, support vector machine (SVM), and neural
network RBFNN, respectively. Classification was evaluated in highly
sensitivity, specificity, and kappa statistical parameters. In
conclusion, the classification results were obtained within short time
period, and the results became better when PCA was used.
Abstract: Glutathione S-transferase was purified from human
erythrocytes and effects of some polyphenols were investigated on
the enzyme activity. The purification procedure was performed on
Glutathione-Agarose affinity chromatography after preparation of
erythrocytes hemolysate with a yield of 81%. The purified enzyme
showed a single band on the SDS-PAGE. The effects of some
poliphenolic compounds such as catechin, dopa, dopamine, progallol
and catechol were examined on the in vitro GST activity. Catechin
was determined to be inhibitor for the enzyme, but others were not
effective on the enzyme as inhibitors or activators. IC50 value -the
concentration of inhibitor which reduces enzyme activity by 50%-
was estimated to be 10 mM. Ki constants were also calculated as 6.38
± 0,70 mM with GSH substrate, and 3.86 ± 0,78 mM with CDNB
substrate using the equations of graphs for the inhibitor, and its
inhibition type was determined as non-competitive.
Abstract: A new and novel approach in medicine is the use of
cold plasma for various applications such as sterilization blood
coagulation and cancer cell treatment. In this paper a pin-to-hole
plasma jet suitable for biological applications is investigated and
characterized and the possibility and feasibility of cancer cell
treatment is evaluated. The characterization includes power
consumption via Lissajous method, thermal behavior of plasma using
Infra-red camera as a novel method, Optical Emission Spectroscopy
(OES) to determine the species that are generated. Treatment of
leukemia cancer cells is also implemented and MTT assay is used to
evaluate viability.
Abstract: Information regarding early onset neonatal sepsis
(EONS) pathogens may vary between regions. Global perspectives
showed Group B Streptococcal (GBS) as the most common causative
pathogens, but the widespread use of intrapartum antibiotics has
changed the pathogens pattern towards gram negative
microorganisms, especially E. coli. Objective of this study is to
describe the pathogens isolated, to assess current treatment and risk
of EONS. Records of 899 neonates born in three General Hospitals
between 2009 until 2012 were retrospectively reviewed. Proven was
found in 22 (3%) neonates. The majority was isolated with gram
positive organisms, 17 (2.3%). All grams positive and most gram
negative organisms showed sensitivity to the tested antibiotics. Only
two rare gram negative organisms showed total resistant. Male was
possible risk of proven EONS. Although proven EONS remains
uncommon in Malaysia, nonetheless, the effect of intrapartum
antibiotics still required continuous surveillance.