Abstract: Medicinal plants are now gaining attractiveness in
treatment of bacterial infections and food preservation. The objective
of this study was to assess antibacterial activity of some medicinal
plants on pathogenic bacteria. Screening of antibacterial activity of
aqueous and methanol extracts of some plants: Jojoba, Ginger, Sage,
Thyme and Clove against Bacillus cereus, Salmonella typhimurium,
Staphylococcus aureus, Clostridium perfringens and Escherichia coli
were investigated. Antibacterial activity was performed by agar
diffusion and disc diffusion method. Jatropha, Jojoba, Clove and
Ginger extracts showed notable bacterial activity in the first
screening step then selected to be tested against Bacillus cereus
(Gram+), Staphylococcus aureus (Gram+) and Salmonella
typhimurium (Gram−) and their effect was compared using
antibiotics as control. Screening results showed potential antibacterial
activity of the tested plant extracts against the screened bacterial
strains. It was found that methanol extracts exhibited higher
antibacterial activity than aqueous extracts. Methanol extract of
Jatropha showed the highest inhibition zone against Staphylococcus
aureus (Gram+) with 24.00 mm diameter, compared to the other
plant extracts followed by clove. Meanwhile, the inhibition zones of
methanol extracts of Jojoba and Ginger were the same (12mm).The
Gram-positive bacteria were found to be more sensitive to aqueous
and methanol extracts than Gram-negative bacteria.
Abstract: Nitrogen fertilizer is the most used and often the most
mismanaged nutrient input. Nitrogen management has tremendous
implications on crop productivity, quality and environmental
stewardship. Sufficient nitrogen is needed to optimum yield and
quality. Soil and in-season plant tissue testing for nitrogen status are
a time consuming and expensive process. Real time sensing of plant
nitrogen status can be a useful tool in managing nitrogen inputs. The
objectives of this project were to assess the reliability of remotely
sensed non-destructive plant nitrogen measurements compared to wet
chemistry data from sampled plant tissue, develop in-season nitrogen
recommendations based on remotely sensed data for improved
nitrogen use efficiency and assess the potential for determining yield
and quality from remotely sensed data. Very good correlations were
observed between early-season remotely sensed crop nitrogen status
and plant nitrogen concentrations and subsequent in-season fertilizer
recommendations. The transmittance/absorbance type meters gave
the most accurate readings. Early in-season fertilizer recommendation
would be to apply 40 kg nitrogen per hectare plus 15 kg nitrogen per
hectare for each unit difference measured with the SPAD meter
between the crop and reference area or 25 kg plus 13 kg per hectare
for each unit difference measured with the CCM 200. Once the crop
was sufficiently fertilized meter readings became inconclusive and
were of no benefit for determining nitrogen status, silage yield and
quality and grain yield and protein.
Abstract: In recent years, the compression of date (Phoenix
dactylifera L.) fruit powders (DP) to obtain date tablets (DT) has
been suggested as a promising form of valorization of non
commercial valuable date fruit (DF) varieties. To further improve
and characterize DT, the present study aims to investigate the
influence of the DP particle size and compression force on some
physical properties of DT. The results show that independently of
particle size, the hardness (y) of tablets increases with the increase of
the compression force (x) following a logarithmic law (y = a ln (bx)
where a and b are the constants of model). Further, a full factorial
design (FFD) at two levels, applied to investigate the erosion %,
reveals that the effects of time and particle size are the same in
absolute value and they are beyond the effect of the compression.
Regarding the disintegration time, the obtained results also by means
of a FFD show that the effect of the compression force exceeds 4
times that of the DP particle size. As final stage, the color parameters
in the CIELab system of DT immediately after their obtaining are
differently influenced by the size of the initial powder.
Abstract: The bean (Phaseolus vulgaris L.) is one of the best
known of the legumes, and it has a long cultivation tradition in Italy.
The territory of “Subappennino Dauno” (southern Italy) is at around
700 m a.s.l. and is predominantly grown with cereals, olive trees and
grapevines. Ecotypes of white beans to eat dry (such as cannellini
beans) are also grown, which are sought for their palatability, high
digestibility, and ease of cooking. However, these are not easy to find
on the market due to their low production in relatively small areas
and on small family farms that use seeds handed down from
generation to generation. The introduction of these ecotypes in plain
areas of the Puglia region would provide an opportunity to promote
the diffusion of this type of bean. To investigate the adaptability of
these ecotypes in plain environments (Cerignola, in southern Italy) a
comparative trial was carried out between three ‘Monti Dauni’
ecotypes (E1, E2, E3) that are native to mountain areas and the
similar commercial variety, ‘Cannellini’. The data provide useful
information about the quantitative and qualitative characteristics of
these ecotypes when grown in lowland environments. Ecotype E3
provided the greatest bean production (2.34 t ha-1) compared to
‘Cannellini’ (1.28 t ha-1) and the other ecotypes (0.55 and 0.40 t ha-1,
for E1 and E2, respectively), due to its greater plant growth and the
larger size of the seed (and thickness, in particular). Finally, ecotype
E2 provided the greatest protein content (31.2%), although not
significantly different from the commercial cultivar ‘Cannellini’
(32.1%).
Abstract: The aim of present study was to monitor the presence
of Trichodina sp. in Rainbow trout, Oncorhynchus mykiss collected
from various fish farms in the western provinces of Iran during
January, 2013- January, 2014. Out of 675 sampled fish 335, (49.16%)
were infested with Trichodina. The highest prevalence was observed
in the spring and winter followed by autumn and summer. In general,
the intensity of infection was low except in cases where outbreaks of
Trichodiniasis endangered the survival of fish in some ponds. In light
infestation Trichodina is usually present on gills, fins and skin of
apparently healthy fish. Clinical signs of Trichodiniasis only appear
on fish with heavy infections and cases of moderate ones that are
usually exposed to one or more stress factors including, rough
handling during transportation from ponds, overcrowdness,
malnutrition, high of free ammonia and low of oxygen concentration.
Clinical signs of Trichodiniasis in sampled fish were sluggish
movement, loss of appetite, black coloration, necrosis and ulcer on
different parts of the body, detached scales and excessive
accumulation of mucous in gill pouches. The most obvious
histopathological changes in diseased fish were sloughing of the
epidermal layer, aggregation of leucocytes and melanine-carrying
cells (between the dermis and hypodermis) and proliferative changes
including hyperplasia and hypertrophy of the epithelial lining cells of
gill filaments which resulted in fusion of secondary lamellae. Control
of Trichodiniasis, has been achieved by formalin bath treatment at a
concentration of 250 ppm for one hour.
Abstract: Mech-Degla, Degla-Beida and Frezza are the date
(Phoenix dactylifera L.) common varieties with a more or less good
availability and feeble trade value. Some morphologic and
physicochemical factors were determined. Results show that the
whole date weight is significantly different (P= 95%) concerning
Mech-Degla and Degla-Beida which are more commercialized than
Frezza whereas the pulp mass proportion in relation to whole fruits is
highest for Frezza (88.28%). Moreover, there is a large variability
concerning the weights and densities of constitutive tissues in each
variety. The white tissue is dominant in Mech-Degla in opposite to
the two other varieties. The variance analyze showed that the
difference in weights between brown and white tissues is significant
(P = 95%) for all studied varieties. Some other morphologic and
chemical proprieties of the whole pulps and their two constitutive
parts (brown or pigmented and white) are also investigated. The
predominance of phenolics in Mech-Degla (4.01g/100g, w.b) and
Frezza (4.96 g/100g, w.b) pulps brown part is the main result
revealed in this study.
Abstract: The study was conducted to evaluate the efficiency of
Garlic and Chili combination solution on control of insect pests in
cabbage crop. The solution was sprayed at different intervals after
transplanting. The efficiency of Garlic and chili combination solution
on cabbage insect pests was measured. Results revealed that Garlic
and chili combination solution was the effectively reduced cabbage
insect pests. On other hand, the spray solution not only reduced the
number of days required for the cabbage growth but also greatly
enhanced the leaf number, head diameter, head weight, and quality of
cabbage. Garlic and chili combination solution have positive effects
on pests reduction and improve growth, yield and quality of cabbage
vegetable.
Abstract: The aim of the paper was to elaborate a novel calculator BasWilCalc, that allows to estimate the actual amount of biomass on the basket willow plantations. The proposed method is based on the results of field experiment conducted during years 2011-2013 on basket willow plantation in the south-western part of Poland. As input data the results of destructive measurements of the diameter, length and weight of willow stems and non-destructive biometric measurements of diameter in the middle of stems and their length during the growing season performed at weekly intervals were used. Performed analysis enabled to develop the algorithm which, due to the fact that energy plantations are of known and constant planting structure, allows to estimate the actual amount of willow basket biomass on the plantation with a given probability and accuracy specified by the model, based on the number of stems measured and the age of the plantation.
Abstract: The lactic acid bacteria (LAB) were isolated from
10 samples of fermented foods (Sa-tor-dong and Bodo) in South
locality of Thailand. The 23 isolates of lactic acid bacteria were
selected, which were exhibited a clear zone and growth on MRS
agar supplemented with CaCO3. All of lactic acid bacteria were
tested on morphological and biochemical. The result showed that
all isolates were Gram’s positive, non-spore forming but only
10 isolates displayed catalase negative. The 10 isolates including
BD1 .1, BD 1.2, BD 2.1, BD2.2, BD 2.3, BD 3.1, BD 4.1, BD 5.2,
ST 4.1 and ST 5.2 were selected for inhibition activity
determination. Only 2 strains (ST 4.1 and BD 2.3) showed
inhibition zone on agar, when using Escherichia coli sp. as target
strain. The ST 4.1 showed highest inhibition zone on agar, which
was selected for probiotic property testing. The ST4.1 isolate
could grow in MRS broth containing a high concentration of
sodium chloride 6%, bile salts 7%, pH 4-10 and vary temperature
at 15-45°C.
Abstract: The mineral bioflotation represents a viable
alternative for the evaluation of new processes benefit alternative.
The adsorption bacteria on minerals surfaces will depend mainly on
the type of the microorganism as well as of the studied mineral
surface. In the current study, adhesion of S. carnosus on coal was
studied. Several methods were used as: DRX, Fourier Transform
Infra-Red (FTIR) adhesion isotherms and kinetic. The main goal is to
recovery of organic matter by the microflotation process on coal
particles with biological reagent (S. carnosus). Adhesion tests
revealed that adhesion took place after of 8 h at pH 9. The results
suggest that the adhesion of bacteria to solid substrates can be
considered an abiotic physicochemical process that is consequently
governed by bacterial surface properties such as their specific surface
area, hydrophobicity and surface functionalities. The greatest coal
fine flotability was of 75%, after 5 min of flotation.
Abstract: Currently, biological control programs in greenhouse
crops involve the use, at the same time, several natural enemies
during the crop cycle. Also, large number of plant species grown in
greenhouses, among them, the used cultivars are also wide. However,
the cultivar effects on entomophagous species efficacy (predators and
parasitoids) have been scarcely studied. A new method had been
developed, using the factitious prey or host Ephestia kuehniella. It
allow us to evaluate, under greenhouse or controlled conditions
(semi-field), the cultivar effects on the entomophagous species
effectiveness. The work was carried out in greenhouse tomato crop. It
has been found the biological and ecological activities of predatory
species (Nesidiocoris tenuis) and egg-parasitoid (Trichogramma
achaeae) can be well represented with the use of the factitious prey
or host; being better in the former than the latter. The data found in
the trial are shown and discussed. The developed method could be
applied to evaluate new plant materials before making available to
farmers as commercial varieties, at low costs and easy use.
Abstract: Natural antimicrobials are used to preserve foods that
can be found in plants, animals, and microorganisms. Antimicrobial
substances are natural or artificial agents that produced by
microorganisms or obtained semi/total chemical synthesis are used at
low concentrations to inhibit the growth of other microorganisms.
Food borne pathogens and spoilage microorganisms are inactivated
by the use of antagonistic microorganisms and their metabolites.
Yeasts can produce toxic proteins or glycoproteins (toxins) that cause
inhibition of sensitive bacteria and yeast species. Antimicrobial
substance producing phenotypes belonging different yeast genus
were isolated from different sources. Toxins secreted by many yeast
strains inhibiting the growth of other yeast strains. These strains show
antimicrobial activity, inhibiting the growth of mold and bacteria.
The effect of antimicrobial agents produced by yeasts can be
extremely fast, and therefore may be used in various treatment
procedures. Rapid inhibition of microorganisms is possibly caused by
microbial cell membrane lipopolysaccharide binding and in
activation (neutralization) effect. Antimicrobial agents inhibit the
target cells via different mechanisms of action.
Abstract: Fibrin degradation is an important part in prevention
or treatment of intravascular thrombosis and cardiovascular diseases.
Plasmin like fibrinolytic enzymes has given new hope to patient with
cardiovascular diseases by treating fibrin aggregation related diseases
with traditional plasminogen activator which have many side effects.
Various researches involving wide range of sources for production of
fibrinolytic proteases, from bacteria, fungi, insects and fermented
foods. But few have looked into endophytic fungi as a potential
source. Sixteen (16) endophytic fungi were isolated from Hibiscus sp.
leaves from six different locations in Shah Alam, Selangor. Only two
endophytic fungi, FH3 and S13 showed positive fibrinolytic protease
activities. FH3 produced 5.78cm and S13 produced 4.48cm on Skim
Milk Agar after 4 days of incubation at 27°C. Fibrinolytic activity
was observed; 3.87cm and 1.82cm diameter clear zone on fibrin plate
of FH3 and S13 respectively. 18srRNA was done for identification of
the isolated fungi with positive fibrinolytic protease. S13 had the
highest similarity (100%) to that of Penicillium citrinum strain TG2
and FH3 had the highest similarity (99%) to that of Fusarium sp.
FW2PhC1, Fusarium sp. 13002, Fusarium sp. 08006, Fusarium
equiseti strain Salicorn 8 and Fungal sp. FCASAn-2. Media
composition variation showed the effects of carbon nitrogen on
protein concentration, where the decrement of 50% of media
composition caused drastic decrease in protease of FH3 from 1.081 to
0.056 and also S13 from 2.946 to 0.198.
Abstract: There are various sources of energies available
worldwide and among them, crude oil plays a vital role. Oil recovery
is achieved using conventional primary and secondary recovery
methods. In-order to recover the remaining residual oil, technologies
like Enhanced Oil Recovery (EOR) are utilized which is also known
as tertiary recovery. Among EOR, Microbial enhanced oil recovery
(MEOR) is a technique which enables the improvement of oil
recovery by injection of bio-surfactant produced by microorganisms.
Bio-surfactant can retrieve unrecoverable oil from the cap rock which
is held by high capillary force. Bio-surfactant is a surface active agent
which can reduce the interfacial tension and reduce viscosity of oil
and thereby oil can be recovered to the surface as the mobility of the
oil is increased. Research in this area has shown promising results
besides the method is echo-friendly and cost effective compared with
other EOR techniques. In our research, on laboratory scale we
produced bio-surfactant using the strain Pseudomonas putida (MTCC
2467) and injected into designed simple sand packed column which
resembles actual petroleum reservoir. The experiment was conducted
in order to determine the efficiency of produced bio-surfactant in oil
recovery. The column was made of plastic material with 10 cm in
length. The diameter was 2.5 cm. The column was packed with fine
sand material. Sand was saturated with brine initially followed by oil
saturation. Water flooding followed by bio-surfactant injection was
done to determine the amount of oil recovered. Further, the injection
of bio-surfactant volume was varied and checked how effectively oil
recovery can be achieved. A comparative study was also done by
injecting Triton X 100 which is one of the chemical surfactant. Since,
bio-surfactant reduced surface and interfacial tension oil can be easily
recovered from the porous sand packed column.
Abstract: Processing tabah bamboo shoot as fermented pickle is
one of the way to increase the shelf life of this bamboo shoot. The
advantage of this shoot is low concentration of hydro cyanic acid
(HCN) make it potential for functional food product. This study
aimed to determine the characteristic of tabah bamboo shoot pickle
such as total of lactic acid bacteria (LAB), pH, total acidity, and
hydro cyanic acid (HCN) content, and also find the LAB’s type
involved during fermentation, and organic acids’ profiles. The pickle
was made by natural fermentation with 6% salt concentration and
fermentation conducted for 13 days.
The result showed during the fermentation time, in the 4th day
LAB’s number was highest as much as 72 x 107 CFU/ml and the
lowest pH was 3.09. We also found decreasing in HCN from 37.8
ppm at the beginning to 20.52 ppm at the end of fermentation
process. The organic acids detected during the fermentation were
lactic acid with the highest concentration was 0.0546 g/100 g and
small amount of acetic acid. By using PCR method, the 18 of LABs
which had rod shape were detected as member of Lactobacillus spp.,
in which 17 strains detected as L. plantarum.
Abstract: Three strains of Vibrio parahaemolyticus (690, BCRC
13023 and BCRC 13025) implicated in food poisoning outbreaks in
Taiwan were subjected to acid adaptation at pH 5.5 for 90 min. The
growth behaviors of acid-adapted and non-adapted V.
parahaemolyticus in the media supplemented with various nitrogen
and carbon sources were investigated. The effects of acid adaptation
on the thermostable direct hemolysin (TDH) secretion and fatty acid
profiles of V. parahaemolyticus were also examined. Results showed
that acid-adapted and non-adapted V. parahaemolyticus 690, BCRC
13023 and BCRC 13025 grew similarly in TSB-3% NaCl and basal
media supplemented with various carbon and nitrogen sources during
incubation period. Higher TDH secretion was noted with V.
parahaemolyticus 690 among the three strains. However, acid-adapted
strains produced less amounts of TDH than non-adapted strains when
they were grown in TSB-3% NaCl. Additionally, acid adaptation
increased the ratio of SFA/USFA in cells of V. parahaemolyticus
strains.