Abstract: This study was conducted to determine effect of water stress on chlorophyll content and chlorophyll fluorescence parameter in young `Dezful- olive trees. Three irrigation regimes (40% ETcrop, 65% ETcrop and 100% ETcrop) were used. After irrigation treatments were applied, some of biochemical parameters including chlorophyll a, b, total chlorophyll, chlorophyll fluorescence and also chlorophyll content index (C.C.I) were measured. Results of Analysis of variance showed that irrigation treatments had significant effect on chlorophylla, total chlorophyll (chl a+b), C.C.I and Fv/Fm ratio. The amount of decreased chlorophyll a and total chlorophyll in plants were received 40% ETcrop were 51.55% and 46.86%, respectively, compared with 100% ETcrop.
Abstract: The antioxidant capability of beverage blends made from cocoa, zobo and ginger with standard antioxidant assay procedures was investigated. The DPPH (2,2-diphenyl-1- picrylhydrazyl) scavenging capacity ranged from 21.2-25.8% in comparison with GSH of 37.1%. The ferric reducing ability was highest in the zobo drink and lowest in ginger. The superoxide scavenging capacity was also highest in the zobo drink followed by the drink with alkalized cocoa. The metal chelating power decreased as the level of zobo in the blends decreases. The chelating power of zobo and ginger were significantly lower than the natural and alkalized cocoa. The 100% zobo drink inhibited linoleic acid till the fifth day while natural and alkalized cocoa as well as the blend with 50% alkalized cocoa inhibited linoleic acid greatly till the sixth day. The finding describes the potential health benefit of the phytochemical antioxidants of cocoa:zobo:ginger beverage blends.
Abstract: The present study has been conducted to characterize
the prophenoloxidase (PPO) system of the desert locust, Schistocerca
gregaria following injection of Bacillus thuringiensis kurstaki (Bt).
The bulk of PPO system was associated with haemocytes and a little
amount was found in plasma. This system was activated by different
activators such as laminarin, lipopolysaccharide (LPS) and trypsin
suggesting that the stimulatory mechanism may involve an enzyme
cascade of one or more associated molecules. These activators did
not activate all the molecules of the cascade. Presence of
phenoloxidase activity (PO) coincides with the appearance of protein
band with molecular weight (MW) 70.154 KD (Kilo Dalton).
Abstract: Present study summarizes the control of Vibrio
alginolyticus infection in hatchery reared Clownfish, Amphiprion
sebae with the extract of the mangrove plant, Avicennia marina.
Fishes with visible symptoms of hemorrhagic spots were chosen and
the genomic DNA of the causative bacterium was isolated and
sequenced based on 16S rDNA gene. The in vitro assay revealed that
a fraction of A. marina leaf extract elucidated with ethyl acetate:
methanol (6:4) showed a high activity (28 mm) at 125 μg/ml
concentrations. About 4 % of the fraction fed along with live V.
alginolyticus was significantly decreased the cumulative mortality
(P
Abstract: This study include the effect of strain and storage
period and their interaction on some quantitative and qualitative traits
and percentages of the egg components in the eggs collected at the
start of production (at age 24 weeks). Eggs were divided into three
storage periods (1, 7 and 14) days under refrigerator temperature (5-
7)0C. Fifty seven eggs obtained randomly from each strain including
Isa Brown and Lohman White. General Linear Model within
SAS programme was used to analyze the collected data
and correlations between the studied traits were calculated for each
strain.Average egg weight (EW), Haugh Unit (HU), yolk index (YI),
yolk % (HP), albumin % (AP) and yolk to albumin ratio (YAR) was
56.629 gm, 87.968 %, 0.493, 22.13%, 67.74% and 32.76
respectively. Egg produced from ISA Brown surpassed those
produced by Lohman White significantly (P
Abstract: Atrazine, a herbicide widely used in sugarcane and corn production, is a frequently detected groundwater contaminant. An atrazine-degrading bacterium, strain KB02, was obtained from long-term atrazine-treated sugarcane field soils in Kanchanaburi province of Thailand. Strain KB02 had a rod-to-coccus morphological cycle during growth. Sequence analysis of the PCR product indicated that the 16S rRNA gene in strain KB02 was ranging from 97-98% identical to the same region in Klebsiella sp. Based on biochemical, physiological analysis and 16S rDNA sequence analysis of one representative isolate, strain KB02, the isolates belong to the genus Klebsiella in the family Enterobacteriaceae. Interestingly that the various primers for atzA, B and C failed to amplify genomic DNA of strain KB02. Whereas the expected PCR product of atzA, B and C were obtained from the reference strain, Arthrobacter sp. strain KU001.
Abstract: The halophilic proteinase showed a maximal activity
at 50°C and pH 9~10, in 20% NaCl and was highly stabilized by
NaCl. It was able to hydrolyse natural actomyosin (NAM), collagen
and anchovy protein. For NAM hydrolysis, the myosin heavy chain
was completely digested by halophilic proteinase as evidenced by the
lowest band intensity remaining, but partially hydrolysed actin. The
SR5-3 proteinase was also capable hydrolyzing two major
components of collagen, β- and α-compounds, effectively. The
degree of hydrolysis (DH) of the halophilic proteinase and
commercial proteinases (Novozyme, Neutrase, chymotrypsin and
Flavourzyme) on the anchovy protein, were compared, and it was
found that the proteinase showed a greater degree of hydrolysis
towards anchovy protein than that from commercial proteinases. DH
of halophilic proteinase was sharply enhanced according to the
increase in the concentration of enzyme from 0.035 U to 0.105 U.
The results warranting that the acceleration of the production of fish
sauce with higher quality, may be achieved by adding of the
halophilic proteinase from this bacterium.
Abstract: Carbonic anhydrases (CAs) has been focused as
biological catalysis for CO2 sequestration process because it can
catalyze the conversion of CO2 to bicarbonate. Here, codon-optimized
sequence of α type-CA cloned from Duneliala species. (DsCAopt) was
constructed, expressed, and characterized. The expression level in E.
coli BL21(DE3) was better for codon-optimized DsCAopt than intact
sequence of DsCAopt. DsCAopt enzyme shows high-stability at pH
7.6/10.0. In final, we demonstrated that in the Ca2+ solution, DsCAopt
enzyme can catalyze well the conversion of CO2 to CaCO3, as the
calcite form.
Abstract: Empty Fruit Bunches (EFB) and Palm Oil Mill
Effluent (POME) are two main wastes from oil palm industries which
contain rich lignocellulose. Degradation of EFB and POME by
microorganisms will produce hydrolytic enzyme which will degrade
cellulose and hemicellulose during composting process. However,
normal composting takes about four to six months to reach maturity.
Hence, application of fungi into compost can shorten the period of
composting. This study identifies the effect of xylanase and cellulase
produced by Aspergillus niger and Trichoderma virens on
composting process using EFB and POME. The degradation of EFB
and POME indicates the lignocellulolytic capacity of Aspergillus
niger and Trichoderma virens with more than 7% decrease in
hemicellulose and more than 25% decrease in cellulose for both
inoculated compost. Inoculation of Aspergillus niger and
Trichoderma virens also increased the enzyme activities during the
composting period compared to the control compost by 21% for both
xylanase and cellulase. Rapid rise in the activities of cellulase and
xylanase was observed by Aspergillus niger with the highest
activities of 14.41 FPU/mg and 3.89 IU/mg, respectively. Increased
activities of cellulase and xylanase also occurred in inoculation of
Trichoderma virens with the highest activities obtained at 13.21
FPU/mg and 4.43 IU/mg, respectively. Therefore, it is evident that
the inoculation of fungi can increase the enzyme activities hence
effectively degrading the EFB and POME.
Abstract: fifteen cultivars of Strawberries (Queen Eliza, Sequia,
Paros, Mcdonance, Selva, Chandler, Mrak, Ten beauty, Aliso, Pajero,
Kordestan, Camarosa, Blackmore, Gaviota and Fresno) were
investigated in 2011, under hydroponic system condition. Yield and
fruit Firmness was determinate. Chemical analyses of soluble solids
content (SSC), titratable acidity (TA), ascorbic acid (AA) and pH
were done. 4 cultivars (Aliso, Selva, Paros and Gaviota) yielded more
than 250 g/plant, while cultivar Black more, Fresno and Kordestan
produced less than 100g/plant. The amounts of fruit firmness
indicated that 'Camarosa' fruit was firmer than others cultivars.
Cultivar 'Fresno' had the highest pH (3.27). Ttitratable acidity varied
from 1.03g/l00g for cultivar 'Sequia' and 'Gaviota' to 1.48g/l00g for
cultivar 'Chandler'. Fresno, Kordestan, Aliso and Chandler showed
the highest soluble solid concentration. Ascorbic acid averaged for
most cultivars between 30.26 and 79.73 mg/100gf.w. Present results
showed that different cultivars of strawberry contain highly variable
in fruit quality.
Abstract: This study aims to screen out and to optimize the
major nutrients for maximum carotenoid production and
antioxidation characteristics by Rhodotorula rubra. It was found that
supplementary of 10 g/l glucose as carbon source, 1 g/l ammonium
sulfate as nitrogen source and 1 g/l yeast extract as growth factor in
the medium provided the better yield of carotenoid content of 30.39
μg/g cell dry weight the amount of antioxidation of Rhodotorula
rubra by DPPH, ABTS and MDA method were 1.463%, 34.21% and
34.09 μmol/l, respectively.
Abstract: In this paper spatial variability of some chemical and
physical soil properties were investigated in mountain rangelands of
Nesho, Mazandaran province, Iran. 110 soil samples from 0-30 cm
depth were taken with systematic method on grid 30×30 m2 in
regions with different vegetation cover and transported to laboratory.
Then soil chemical and physical parameters including Acidity (pH),
Electrical conductivity, Caco3, Bulk density, Particle density, total
phosphorus, total Nitrogen, available potassium, Organic matter,
Saturation moisture, Soil texture (percentage of sand, silt and clay),
Sodium, Calcium, magnesium were measured in laboratory. Data
normalization was performed then was done statistical analysis for
description of soil properties and geostatistical analysis for indication
spatial correlation between these properties and were perpetrated
maps of spatial distribution of soil properties using Kriging method.
Results indicated that in the study area Saturation moisture and
percentage of Sand had highest and lowest spatial correlation
respectively.