Abstract: This study evaluated the microbiological quality
and the sensory characteristics of carp fillets processed by the
sousvide method when stored at 2 and 10 °C. Four different
combinations of sauced–storage were studied then stored at 2 or 10
°C was evaluate periodically sensory, microbiological and
chemical quality. Batches stored at 2 °C had lower growth rates of
mesophiles and psychrotrophs. Moreover, these counts decreased
by increasing the heating temperature and time. Staphylococcus
aureus, Bacillus cereus, Clostridium perfringens and Listeria
monocytogenes were not found in any of the samples. The heat
treatment of 90 °C for 15 min and sauced was the most effective to
ensure the safety and extend the shelf-life of sousvide carp
preserving its sensory characteristics. This study establishes the
microbiological quality of sous vide carp and emphasizes the
relevance of the raw materials, heat treatment and storage
temperature to ensure the safety of the product.
Abstract: In order to study the effect of plant density and
competition of wheat with field bindweed (Convolvulus arvensis) on
yield and agronomical properties of wheat(Triticum Sativum) in
irrigated conditions, a factorial experiment as the base of complete
randomize block design in three replication was conducted at the
field of Kamalvand in khoramabad (Lorestan) region of Iran during
2008-2009. Three plant density (Factor A=200, 230 and 260kg/ha)
three cultivar (Factor B=Bahar,Pishtaz and Alvand) and weed control
(Factor C= control and no control of weeds)were assigned in
experiment. Results show that: Plant density had significant effect
(statistically) on seed yield, 1000 seed weight, weed density and dry
weight of weeds, seed yield and harvest index had been meaningful
effect for cultivars. The interaction between plant density and
cultivars for weed density, seed yield, thousand seed weight and
harvest index were significant. 260 kg/ha (plant density) of wheat had
more effect on increasing of seed yield in Bahar cultivar wheat in
khoramabad region of Iran.
Abstract: Prickly pear (Opuntia spp) fruit has received renewed
interest since it contains a betalain pigment that has an attractive
purple colour for the production of juice. Prickly pear juice was
prepared by homogenizing the fruit and treating the pulp with 48 g of
pectinase from Aspergillus niger. Titratable acidity was determined
by diluting 10 ml prickly pear juice with 90 ml deionized water and
titrating to pH 8.2 with 0.1 N NaOH. Brix was measured using a
refractometer and ascorbic acid content assayed
spectrophotometrically. Colour variation was determined
colorimetrically (Hunter L.a.b.). Hunter L.a.b. analysis showed that
the red purple colour of prickly pear juice had been affected by juice
treatments. This was indicated by low light values of colour
difference meter (CDML*), hue, CDMa* and CDMb* values. It was
observed that non-treated prickly pear juice had a high (colour
difference meter of light) CDML* of 3.9 compared to juice
treatments (range 3.29 to 2.14). The CDML* significantly (p
Abstract: The increments of aromatic structures are widely used to monitor the degree of humification. Compost derived from mix manures mixed with agricultural wastes was studied. The compost collected at day 0, 7, 14, 21, 28, 35, 49, 77, 91, 105, and 119 was divided into 3 stages, initial stage at day 0, thermophilic stage during day 1-48, and mature stage during day 49-119. The change of highest absorptions at wavelength range between 210-235 nm during day 0- 49 implied that small molecules such as nitrates and carboxylic occurred faster than the aromatic molecules that were found at wavelength around 280 nm. The ratio of electron-transfer band at wavelength 253 nm by the benzonoid band at wavelength 230 nm (E253/E230) also gradually increased during the fermenting period indicating the presence of O-containing functional groups. This was in agreement with the shift change from aliphatic to aromatic structures as shown by the relationship with C/N and H/C ratios (r = - 0.631 and -0.717, p< 0.05) since both were decreasing. Although the amounts of humic acid (HA) were not different much during the humification process, the UV spectral deconvolution showed better qualitative characteristics to help in determining the compost quality. From this study, the compost should be used at day 49 and should not be kept longer than 3 months otherwise the quality of HA would decline regardless of the amounts of HA that might be rising. This implied that other processes, such as mineralization had an influence on the humification process changing HA-s structure and its qualities.
Abstract: The aim of the present study was to investigate the
chemical and biological properties of local cowpea seed protein
cultivated in Gizan region. The results showed that the cowpea and
its products contain high level of protein (22.9-77.6%), high
carbohydrates (9.4-64.3%) and low fats (0.1-0.3%). The trypsin and
chymotrypsin activities were found to be 32.2 and 15.2 units,
respectively. These activities were not affected in both defatted and
protein concentrate whereas they were significantly reduced in
isolated protein and cooked samples. The phytate content of cooked
and concentrated cowpea samples varied from 0.25% -0.32%,
respectively. Tannin content was found to be 0.4% and 0.23% for
cooked and raw samples, respectively. The in vitro protein
digestibility was very high in cowpea seeds (75.04-78.76%). The
biological evaluation using rats showed that the group fed with
animal feed containing casein gain more weight than those fed with
that containing cowpea. However, the group fed with cooked cowpea
gain more weight than those fed with uncooked cowpea. On the
other hand, in vivo digestion showed high value (98.33%) among the
group consumed casein compared to other groups those consumed
cowpea contains feed. This could be attributed to low antinutritional
factors in casein contains feed compared to those of cowpea contains
feed because cooking significantly increased the digestion rate
(80.8% to 83.5%) of cowpea contains feed. Furthermore, the
biological evaluation was high (91.67%) of casein containing feed
compared to that of cowpea containing feed (80.83%-87.5%). The
net protein utilization (NPU) was higher (89.67%) in the group fed
with casein containing feed than that of cowpea containing feed
(56.33%-69.67%).
Abstract: Principally, plants grown in soilless culture may be
attacked by the same pests and diseases as cultivated traditionally in
soil. The most destructive phytopathogens are fungi, such as
Phythium, Phytophthora and Fusarium, followed by viruses, bacteria
and nematodes. We investigated effect of carbon nanotube filters on
disease management of soilless culture. Tomato seedlings transplant
in plastic pots filled with a soilless media of vermiculite. The crop
irrigated and fertilized using a hydroponic nutrient solution. We used
carbon nanotube filters for nutrient solution disinfection. Our results
show that carbon nanotube filtration significantly reduces pathogens
on tomato plants. Fungal elimination (Fusarium oxysporum and
Pythium spp.) was usually successful at about 96 to 99.9% all over
the cultural season. It is seem that in tomato soilless culture,
nanofiltration constitutes a reliable method that allows control of the
development of diseases caused by pathogenic fungi
Abstract: Our results showed that for the growth of qualitative
seedling and vegetative raw material of ðó. marschallianus Willd. and
T. serphyllum L. it is more profitable to use the in vitro and
hydroponics combined method. In in vitro culture it is possible to do
micro-propagation whole year with 98-99% rhizogenesis. 30000
micro-plants were obtained from one explant during 9 months.
Hydroponic conditions provide the necessary microclimate for
microplants where the survival rate without acclimatization was
93.3%. The essential oil content in hydroponic dry herb of both
species in vegetative and blossom phase was 1.3% whereas in wild
plants it was 1.2%, the content of extractive substances and vitamin
C also exceeded wild plants. Our biochemical and radiochemical
investigations indicated that the medicinal raw materials obtained
from hydroponic and wild plants of Thymus species correspond to
the demands of SPh XI, and the content of artificial radionuclides
does not exceed the MACL.
Abstract: Experiments were carried out at the Faculty of Food
Technology of Latvia University of Agriculture (LLU). Soft cheese
Kleo produced in Latvia was packed in a biodegradable PLA without
barrierproperties and VC999 BioPack lidding film PLA, coated with
a barrier of pure silicon oxide (SiOx) and in combination with
modified atmosphere (MAP) the influence on the shelf life was
investigated and compared with some conventional (OPP, PE/PA,
PE/OPA and Multibarrier 60) polymer film impact. Modified
atmosphere consisted of carbon dioxide CO2 (E 290) 30% and
nitrogen N2 (E 941) 70%. The analyzable samples were stored at the
temperature of +4.0±0.5 °C up to 32 days- and analyzed before
packaging and in the 0, 5th, 11th, 15th, 18th, 22nd, 25th, 29th and 32nd
day of storage. The shelf life was extended along to 32 days, good
outside appearance and lactic acid aroma was observed.
Abstract: This study was conducted to investigate the optimum
levels of glutamine (Gln) supplementation in broiler diets. A total of
32 one-day-old male chicks with initial body weight 41.5 g were
segregated into 4 groups (8 chicks per group) and subsequently
distributed to individual cages. Feed and water were provided ad
libitum for 21 days. Four dietary treatments were as follows: control
and supplemented Gln at 1, 2 and 3%, respectively. The results found
that the addition Gln had no negative effects on dry matter, organic
matter, ash digestibility or nitrogen retention. Birds fed with 1% Gln
had significantly higher villi wide and villi height : crypt depth ratio
in duodenum than the control chicks and 2 and 3% Gln chicks. It is
suggested that the addition of Gln at 1% indicated a beneficial effect
on improving small intestinal morphology, in addition Gln may
stimulate immune organ development of broiler chickens.
Abstract: The phylogenetic analysis using the most conservative
portions of 18S rRNA gene revealed the phylogenetic relationship
among the two populations where DNA divergence showed that the
nucleotides diversity value were -0.00838 for the Tanjung Dawai,
Kedah and -0.00708 for the Cherating, Pahang populations
respectively. The net nucleotide divergence among populations (Da)
was -0.0073 indicating a low polymorphism among the populations
studied. Total number of mutations in the Tanjung Dawai, Kedah
samples was higher than Cherating, Pahang samples, which are 73 and
59 respectively while shared mutations across the populations were 8,
and reveal the evolutionary in the genome of Malaysian T. gigas. The
tree topology of both populations inferred using Neigbour-joining
method by comparing 1791 bp of partial 18S rRNA sequence revealed
that T. gigas haplotypes were clustered into seven clades, suggesting
that they are genetically diverse among populations which derived
from a common ancestor.
Abstract: Humic acids (HAs) have been shown to activate some
ion uptakes along with stimulating the lateral roots at effective
concentration of micronutrients. However, the effects of HA on ion
adsorption by plant roots are not easily explainable due to the
varieties of HAs that differ from origins. Therefore, this study was
aimed to investigate the effect of various concentrations of HA
obtained from the compost derived from mix manures and some
agricultural wastes on the growth of eggplant seedlings (Solanum
melongena L. cv. Chao Praya) in tissue cultures at low nutrient level.
Egg plant seeds were surfaced sterilized and germinated in ½
Murashige and Skoog medium (MS) without HA added or in ¼ MS
supplemented with 0, 25, 50, 75 and 100 ppm of HAs. Then, they
were cultured for 4 weeks under the controlled environment. The
results showed that seedlings grown on ¼MS supplemented with
HAs at the concentration of 25 and 50 ppm had the average plant
heights (2.49 and 2.28 cm, respectively) higher than the other
treatments. Both treatments also significantly showed the maximum
average fresh and dry weights (p
Abstract: Reverse engineering of full-genomic interaction networks based on compendia of expression data has been successfully applied for a number of model organisms. This study adapts these approaches for an important non-model organism: The major human fungal pathogen Candida albicans. During the infection process, the pathogen can adapt to a wide range of environmental niches and reversibly changes its growth form. Given the importance of these processes, it is important to know how they are regulated. This study presents a reverse engineering strategy able to infer fullgenomic interaction networks for C. albicans based on a linear regression, utilizing the sparseness criterion (LASSO). To overcome the limited amount of expression data and small number of known interactions, we utilize different prior-knowledge sources guiding the network inference to a knowledge driven solution. Since, no database of known interactions for C. albicans exists, we use a textmining system which utilizes full-text research papers to identify known regulatory interactions. By comparing with these known regulatory interactions, we find an optimal value for global modelling parameters weighting the influence of the sparseness criterion and the prior-knowledge. Furthermore, we show that soft integration of prior-knowledge additionally improves the performance. Finally, we compare the performance of our approach to state of the art network inference approaches.
Abstract: An experiment was conducted to determine the effect
of the rearing system on growth performance, carcass yield,
hematological parameters, and feather pecking damage of Thai
indigenous chickens. Three hundred and sixty 1-d-old chicks were
randomly assigned to 2 treatments: indoor treatment and outdoor
access treatment. In the indoor treatment, the chickens were housed
in floor pens (5 birds/m2). In the outdoor access treatment, the
chickens were housed in a similar indoor house; in addition, they also
had an outdoor grass paddock (1 bird/m2). All birds were provided
with same diet and were raised for 16 wk of age. The results showed
that growth performance and carcass yield were not different among
treatment (P>0.05). Outdoor access had no effect on hematological
parameters (P>0.05). However, the feather pecking damage of the
chickens in the outdoor access treatment was lower than that of the
chickens in the indoor treatment (P
Abstract: The research object was apple-black currant
marmalade candies. Experiments were carried out at the Faculty of
Food Technology of the Latvia University of Agriculture. An active
packaging in combination with modified atmosphere (MAP, CO2
100%) was examined and compared with traditional packaging in air
ambiance. Polymer Multibarrier 60 and paper bags were used.
Influence of iron based oxygen absorber in sachets of 500 cc
obtained from Mitsubishi Gas Chemical Europe Ageless® was tested
on the quality during the shelf of marmalade. Samples of 80±5 g
were packaged in polymer pouches (110 mm x 110 mm),
hermetically sealed by MULTIVAC C300 vacuum chamber machine,
and stored in room temperature +20.0±1.0 °C. The physiochemical
properties – weight losses, moisture content, hardness, aw, pH, colour,
changes of atmosphere content (CO2 and O2) in headspace of packs,
and microbial conditions were analysed before packaging and in the
1st, 3rd , 5th, 8th, 11th and 15th weeks of storage.
Abstract: Traditionally, Yemini Sidr honey has been reported to
cure liver problems, stomach ulcers, and respiratory disorders. In this
experiment, we evaluated Yemeni Sidr honey for its ability to protect
inflammations caused by acetic acid and formalin -induced writhing,
carrageenan and histamine-induced paw oedema in experimental rat
model. Hyperpyrexia, membrane stabilizing activity, and
phytochemical screening of the honey was also examined. Yemini
Sidr Honey at (100, 200 and 500 mg/kg) exhibited a concentration
dependant inhibition of acetic acid induced and formalin induced
writhing, paw oedema induced by carrageenan & histamine, and
hyperpyrexia induced by brewer's yeast, it also inhibited membrane
stabilizing activity. Phytochemical screenings of the honey reveal the
presence of flavonoids, steroid, alkaloids, saponins and tannins. This
study suggested that Yemeni Sidr honey possess very strong antiinflammatory,
analgesic and antipyretic effects and these effects
would be a result of the phytochemicals present.
Abstract: Optimization of extraction of phenolic compounds
from Avicennia marina using response surface methodology was
carried out during the present study. Five levels, three factors
rotatable design (CCRD) was utilized to examine the optimum
combination of extraction variables based on the TPC of Avicennia
marina leaves. The best combination of response function was 78.41
°C, drying temperature; 26.18°C; extraction temperature and 36.53
minutes of extraction time. However, the procedure can be promptly
extended to the study of several others pharmaceutical processes like
purification of bioactive substances, drying of extracts and
development of the pharmaceutical dosage forms for the benefit of
consumers.
Abstract: The objective was to determine the single gene and
interaction effect of composite genotype of beta-kappa casein and
DGAT1 gene on milk yield (MY) and milk composition, content of
milk fat (%FAT), milk protein (%PRO), solid not fat (%SNF), and
total solid (%TS) in crossbred Holstein cows. Two hundred and
thirty- one cows were genotyped with PCR-RFLP for DGAT1 and
composite genotype data of beta-kappa casein from previous work
were used. Two model, (1), and (2), was used to estimate single gene
effect, and interaction effect on the traits, respectively. The
significance of interaction effects on all traits were detected. Most
traits have consistent pattern of significant when model (1), and (2)
were compared, except the effect of composite genotype of betakappa
casein on %FAT, and the effect of DGAT1 on MY, which the
significant difference was detected in only model (1).The results
suggested that when the optimum of all traits was necessary,
interaction effect should be concerned.
Abstract: A study was conducted to determine the effect of
feeding glycerol on dairy cows performance. Twenty four Holstein
Friesian crossbred (>87.5% Holstein Friesian) lactating dairy cows in
early lactation; averaging 13+2.4 kg of milk, 64+45 days in milk,
55+16 months old and 325+26 kg live weight, were stratified for
milk yield, days in milk, age, stage of lactation and body weight, and
then randomly allocated to three treatment groups. All cows were fed
approximate 8 kg of concentrate together with ad libitum corn silage
and freely access to clean water. Nil or 150 and 300g of glycerol
were supplemented to the cows according to treatment groups. All
cows consumed similar concentrate, corn silage and total DM and
NELP. There were no significant differences in DM intake, CP intake,
NELP intake, milk and milk composition yields. All cows had similar
fat, protein, lactose, solid not fat and total solid percentage. All cows
gain similar live weight. The present study indicated that,
supplementation of glycerol did not enhance milk yield, milk
composition and live weight change.
Abstract: Foodborne Salmonella infections have become a
major problem world wide. Salmonellosis transmitted from fish are
quite common. Established quality control measures exist for export
oriented fish, none exists for fish consumed locally. This study aimed
at characterization of Salmonella isolated from Nile tilapia . The
study was carried out in selected beaches along L. Victoria in
Western Kenya between March and June 2007. One hundred and
twenty fish specimens were collected. Salmonella isolates were
confirmed using serotyping, biochemical testing in addition to malic
acid dehydrogenase (mdh) and fliC gene sequencing. Twenty
Salmonella isolates were confirmed by mdh gene sequencing. Nine
(9) were S. enterica serotype typhimurium, four (4) were S. enterica
Serotype, enteritidis and seven (7) were S. enterica serotype typhi.
Nile tilapia have a role in transmission of Salmonellosis in the study
area, poor sanitation was a major cause of pollution at the beach
inshore waters.