Abstract: Cattle manure and mineral fertilizers are two source
of Nitrogen, which can affect the growth and quantity of potato. In
this research the effects of the use of cattle manure (5, 10, 15 and 20
ton ha-1), Nitrogen fertilizer (50, 100 and 150 kg N ha-1) and their
interaction on potato growth were evaluated during field
experiments in 2008 with the help of Randomized Complete Block
(RCB) with the factorial arrangement of three experimental
replications in Iran. At the 75 th day after emergence, dry weight of
Shoots, leaf area index (LAI) and plant height were recorded. Results
showed that, dry weight of Shoots, LAI and plant height increased
linearly and very significantly in response to the application of
manure and Nitrogen fertilizer. While the interaction between
manure and Nitrogen fertilizer just on the LAI and plant height was
significant, somehow the maximum amount of plant height( 73 cm)
was obtained by using 150 kg Nitrogen + 15 tons of manure per
hectare, and maximum LAI ( 5.36) was obtained by using 150 kg
Nitrogen + 20 tons of manure per hectare. Also in this experiment
maximum tuber yield (36.8 tons ha-1) was obtained by the utilization
of 150 kg Nitrogen per hectare + 20 tons manure.
Abstract: The growing health hazardous impact of arsenic (As)
contamination in environment is the impetus of the present
investigation. Application of lactic acid bacteria (LAB) for the
removal of toxic and heavy metals from water has been reported.
This study was performed in order to isolate and characterize the Asresistant
LAB from mud and sludge samples for using as efficient As
uptaking probiotic. Isolation of As-resistant LAB colonies was
performed by spread plate technique using bromocresol purple
impregnated-MRS (BP-MRS) agar media provided with As @ 50
μg/ml. Isolated LAB were employed for probiotic characterization
process, acid and bile tolerance, lactic acid production, antibacterial
activity and antibiotic tolerance assays. After As-resistant and
removal characterizations, the LAB were identified using 16S rDNA
sequencing. A total of 103 isolates were identified as As-resistant
strains of LAB. The survival of 6 strains (As99-1, As100-2, As101-3,
As102-4, As105-7, and As112-9) was found after passing through the
sequential probiotic characterizations. Resistant pattern pronounced
hollow zones at As concentration >2000 μg/ml in As99-1, As100-2,
and As101-3 LAB strains, whereas it was found at ~1000 μg/ml in
rest 3 strains. Among 6 strains, the As uptake efficiency of As102-4
(0.006 μg/h/mg wet weight of cell) was higher (17 – 209%)
compared to remaining LAB. 16S rDNA sequencing data of 3 (As99-
1, As100-2, and As101-3) and 3 (As102-4, As105-7, and As112-9)
LAB strains clearly showed 97 to 99% (340 bp) homology to
Pediococcus dextrinicus and Pediococcus acidilactici, respectively.
Though, there was no correlation between the metal resistant and
removal efficiency of LAB examined but identified elevated As
removing LAB would probably be a potential As uptaking probiotic
agent. Since present experiment concerned with only As removal
from pure water, As removal and removal mechanism in natural
condition of intestinal milieu should be assessed in future studies.
Abstract: A total of twenty tensile biopsies were collected from
children undergoing tonsillectomy from teaching hospital ENT
department and Kurdistan private hospital in sulaimani city. All
biopsies were homogenized and cultured; the obtained bacterial
isolates were purified and identified by biochemical tests and VITEK
2 compact system. Among the twenty studied samples, only one
Pseudomonas putida with probability of 99% was isolated.
Antimicrobial susceptibility was carried out by disk diffusion
method, Pseudomonas putida showed resistance to all antibiotics
used except vancomycin. The isolate further subjected to PCR and
DNA sequence analysis of blaVIM gene using different set of primers
for different regions of VIM gene. The results were found to be PCR
positive for the blaVIM gene. To determine the sequence of blaVIM
gene, DNA sequencing performed. Sequence alignment of blaVIM
gene with previously recorded blaVIM gene in NCBI- database showed
that P. putida isolate have different blaVIM gene.
Abstract: In this research the Preparation of Land use map of
scanner LISS III satellite data, belonging to the IRS in the Aghche
region in Isfahan province, is studied carefully. For this purpose, the
IRS satellite images of August 2008 and various land preparation
uses in region including rangelands, irrigation farming, dry farming,
gardens and urban areas were separated and identified. Therefore, the
GPS and Erdas Imaging software were used and three methods of
Maximum Likelihood, Mahalanobis Distance and Minimum Distance
were analyzed. In each of these methods, matrix error and Kappa
index were calculated and accuracy of each method, based on
percentages: 53.13, 56.64 and 48.44, were obtained respectively.
Considering the low accuracy of these methods in separation of land
preparation use, the visual interpretation of the map was used.
Finally, regional visits of 150 points were noted at random and no
error was observed. It shows that the map prepared by visual
interpretation is in high accuracy. Although the probable errors due
to visual interpretation and geometric correction might happen but
the desired accuracy of the map which is more than 85 percent is
reliable.
Abstract: Vinegar or sour wine is a product of alcoholic and
subsequent acetous fermentation of sugary precursors derived from
several fruits or starchy substrates. This delicious food additive and
supplement contains not less than 4 grams of acetic acid in 100 cubic
centimeters at 20°C. Among the large number of bacteria that are
able to produce acetic acid, only few genera are used in vinegar
industry most significant of which are Acetobacter and
Gluconobacter. In this research we isolated and identified an
Acetobacter strain from Iranian apricot, a very delicious and sensitive
summer fruit to decay, we gathered from fruit's stores in Isfahan,
Iran. The main culture media we used were Carr, GYC, Frateur and
an industrial medium for vinegar production. We isolated this strain
using a novel miniature fermentor we made at Pars Yeema
Biotechnologists Co., Isfahan Science and Technology Town (ISTT),
Isfahan, Iran. The microscopic examinations of isolated strain from
Iranian apricot showed gram negative rods to cocobacilli. Their
catalase reaction was positive and oxidase reaction was negative and
could ferment ethanol to acetic acid. Also it showed an acceptable
growth in 5%, 7% and 9% ethanol concentrations at 30°C using
modified Carr media after 24, 48 and 96 hours incubation
respectively. According to its tolerance against high concentrations of
ethanol after four days incubation and its high acetic acid production,
8.53%, after 144 hours, this strain could be considered as a suitable
industrial strain for a production of a new type of vinegar, apricot
vinegar, with a new and delicious taste. In conclusion this is the first
report of isolation and identification of an Acetobacter strain from
Iranian apricot with a very good tolerance against high ethanol
concentrations as well as high acetic acid productivity in an
acceptable incubation period of time industrially. This strain could be
used in vinegar industry to convert apricot spoilage to a beneficiary
product and mentioned characteristics have made it as an amenable
strain in food and agricultural biotechnology.
Abstract: Plant growth is affected by the osmotic stress as well as toxicity of salt in leaves. In order to study of salt stress effects on stomatal conductance and growth rate and relationship between them as wells osmotic and Na+-specific effects on these traits, four bread wheat genotypes differing in salt tolerance were selected. Salinity was applied when the leaf 4 was fully expanded. Sodium (Na+) concentrations in flag leaf blade at 3 salinity levels (0, 100 and 200 mM NaCl) were measured. Salt-tolerant genotypes showed higher stomatal conductance and growth rate compared to salt-sensitive ones. After 10 and 20 days exposure to salt, stomatal conductance and relative growth rate were reduced, but the reduction was greater in sensitive genotypes. Growth rate was reduced severely in the first period (1-10 days) of salt commencements and it was due to osmotic effect of salt not Na+ toxicity. In the second period (11-20 days) after salt treatment growth reduced only when salt accumulated to toxic concentrations in the leaves. A positive relationship between stomatal conductance and relative growth rate showed that stomatal conductance can be a reliable indicator of growth rate, and finally can be considered as a sensitive indicator of the osmotic stress. It seems 20 days after salinity, the major effect of salt, especially at low to moderate salinity levels on growth properties was due to the osmotic effect of salt, not to Na+-specific effects within the plant.
Abstract: The experiment was performed to study the
relationship between excreta viscosity and Nitrogen-corrected true
metabolisable energy quantities of soybean meals using conventional
addition method (CAM) in adult cockerels for 7 d: a 3-d preexperiment
and a 4-d experiment period. Results indicated that
differences between the excreta viscosity values were (P
Abstract: The effect of seed inoculation by VA- mycorrhiza and
different levels of phosphorus fertilizer on growth and yield of
sunflower (Azargol cultivar) was studied in experiment farm of
Islamic Azad University, Karaj Branch during 2008 growing season.
The experiment treatments were arranged in factorial based on a
complete randomized block design with three replications. Four
phosphorus fertilizer levels of 25%, 50% 75% and 100% P
recommended with two levels of Mycorrhiza: with and without
Mycorrhiza (control) were assigned in a factorial combination.
Results showed that head diameter, number of seeds in head, seed
yield and oil yield were significantly higher in inoculated plants than
in non-inoculated plants. Head diameter, number of seeds in head,
1000 seeds weight, biological yield, seed yield and oil yield increased
with increasing P level above 75% P recommended in non-inoculated
plants, whereas no significant difference was observed between 75%
and 100% P recommended. The positive effect of mycorrhizal
inoculation decreased with increasing P levels due to decreased
percent root colonization at higher P levels. According to the results
of this experiment, application of mycorrhiza in present of 50% P
recommended had an appropriate performance and could increase
seed yield and oil production to an acceptable level, so it could be
considered as a suitable substitute for chemical phosphorus fertilizer
in organic agricultural systems.
Abstract: A thirty Rahmani weaned male lambs of average body weight (27.28±1.40 kg) were randomly allotted to three similar groups, ten lambs in each, to study the benefit of commercial feed additives Tonilisat (Saccharomyces cerevisiae) and Roemin W2 (Lactobacillus acidophilus, Lactobacillus thermophilus, Bifidobacterium and Lactose) as growth promoters on lambs performance, digestibility, rumen activity and some blood constituents. The experiment lasted about 107 days. Three experimental groups were allotted as control group: received the basal ration, T1 group: received the basal ration supplemented with Tonilisat as (0.5kg/ ton concentrate feed mixture) and T2 group: received the basal ration supplemented with Roemin W2 (1kg/ ton concentrate feed mixture). Our study revealed that addition of Tonilisat significantly increased digestion coefficient of crude protein than that of the control group, Furthermore, the supplementation of Tonilisat or Roemin W2 increased (p
Abstract: Competitive relationships among Bradyrhizobium
japonicum USDA serogroup 123, 122 and 138 were screened versus
the standard commercial soybean variety Williams and two
introductions P1 377578 "671" in a field trial. Displacement of strain
123 by an effective strain should improved N2 fixation. Root nodules
were collected and strain occupancy percentage was determined
using strain specific fluorescent antibodies technique. As anticipated
the strain USDA 123 dominated 92% of nodules due to the high
affinity between the host and the symbiont. This dominance was
consistent and not changed materially either by inoculation practice
or by introducing new strainan. The interrelationship between the
genotype Williams and serogroup 122 & 138 was found very weak
although the cell density of the strain in the rhizosphere area was
equal. On the other hand, the nodule occupancy of genotypes 671 and
166 with rhizobia serogroup 123 was almost diminished to zero. .
The data further exhibited that the genotypes P1 671 and P1 166 have
high affinity to colonize with strains 122 and 138 whereas Williams
was highly promiscuous to strain 123.
Abstract: The effects of chitosan, a biodegradable polymer,
were studied in Grammatophyllum speciosum protocorm-like bodies
(PLBs) in vitro culture. The chitosan concentration of 0, 5, 10, 15,
20, 25, 50 or 100 mg/l were supplemented in half-strength Murashige
and Skoog (1/2 MS) liquid or on agar media containing 2% (w/v)
sucrose. The results showed that liquid medium supplemented with
15 mg/l chitosan showed the highest relative growth rate (7-fold
increase) of PLBs. On 1/2 MS agar medium supplemented with 25
mg/l chitosan gave the highest relative growth rate (4-fold increase).
The relative growth rate of G. speciosum PLBs on agar medium was
significantly lower than that in liquid medium. Moreover, chitosan,
supplemented to agar medium promoted shoot formation but not
rooting. However, supplementation at too high a level, such as 100
mg/l can inhibit growth and kill PLBs.
Abstract: To investigate effect of salt stress on Chlorophyll
fluorescence four cultivars (fong,star,chamran and kharchia) of wheat
(Triticum aestivum) plants subjected to salinity levels ( control,8,12
and 16 dsm-1 ) from one week after emergence to the end of stem
elongation under greenhouse condition . results showed that quantum
yield of photosystem II from light adopted leaves (ΦPSII),
Photochemical quenching (qP) ,quantum yield of dark adopted leaves
(fv/fm) and non photochemical quenching (NPq) were affected by
salt stress . Salinity levels affected photosynthetic rate. Star and fong
cultivars showed minimum and maximum levels of photosynthetic
rate in respectively. Minimum photosynthetic rate differences
between levels of salinity were shown in Kharchia. Shoot dry matter
of all cultivars decreased by increasing salinity levels. Results
showed that non photochemical quenching by salinity levels attribute
to the decreases in shoot dry matter.
Abstract: Efficient utilization of existing water is a pressing
need for Pakistan. Due to rising population, reduction in present
storage capacity and poor delivery efficiency of 30 to 40% from
canal. A study to evaluate an irrigation system in the cotton-wheat
zone of Pakistan, after the watercourse lining was conducted. The
study is made on the basis of cropping pattern and salinity to evaluate
the system. This study employed an index-based approach of using
Geographic information system with field data. The satellite images
of different years were use to examine the effective area. Several
combinations of the ratio of signals received in different spectral
bands were used for development of this index. Near Infrared and
Thermal IR spectral bands proved to be most effective as this
combination helped easy detection of salt affected area and cropping
pattern of the study area. Result showed that 9.97% area under
salinity in 1992, 9.17% in 2000 and it left 2.29% in year 2005.
Similarly in 1992, 45% area is under vegetation it improves to 56%
and 65% in 2000 and 2005 respectively. On the basis of these results
evaluation is done 30% performance is increase after the watercourse
improvement.
Abstract: A study was carried out at the Rice Research Institute of Iran (RRII) to investigate the effect of rollers differential peripheral speed of commercial rubber roll husker and paddy moisture content on the husking index and percentage of broken rice. The experiment was conducted at six levels of rollers differential speed (1.5, 2.2, 2.9, 3.6, 4.3 and 5 m/s) and three levels of paddy moisture content (8-9, 10-11 and 12-13% w.b.). Two common paddy varieties namely, Binam and Khazer, were selected for this study. Results revealed that the effect of rollers differential speed and moisture content significantly (P
Abstract: Dredged sediment (DS) was utilized as source of
silt-clay and organic matter in artificially prepared eelgrass substrates with mountain sand (MS) as the sand media. Addition of DS showed
improved growth of eelgrass in the mixed substrates. Increase in added
DS up to 15% silt-clay showed increased shoot growth but additional
DS in 20% silt-clay mixture didn-t result to further increase in eelgrass
growth. Improved root establishment were also found for plants in pots
with added DS as shown by the increased resistance to uprooting, increased number of rhizome nodes and longer roots. Results demonstrated that addition of DS may be beneficial to eelgrass up to a
certain extent only and too much of it might be harmful to eelgrass plants.
Abstract: In this study, in order to clarify wind-induced
phenomena, especially vertical mixing of density stratification in a
closed water area with floating-leaved plants, we conducted hydraulic
experiments on wind flow characteristics, wind wave characteristics,
entrainment phenomena and turbulent structure by using a wind tunnel
test tank and simulated floating-leaved plants. From the experimental
results of wind flow and wind wave characteristics, we quantified the
impact of the occupancy rate of the plants on their resistance
characteristics. From the experimental results of entrainment
phenomena, we defined the parameter that could explain the
magnitude of mixing between the density stratifications, and
quantified the impact of the occupancy rate on vertical mixing
between stratifications. From the experimental results of the turbulent
structure of the upper layer, we clarified the differences in small-scale
turbulence components at each occupancy rate and quantified the
impact of the occupancy rate on the turbulence characteristics. For a
summary of this study, we theoretically quantified wind-induced
entrainment phenomena in a closed water area with luxuriant growth
of floating-leaved plants. The results indicated that the impact of
luxuriant growth of floating-leaved plants in a closed water body
could be seen in the difference in small-scale fluid characteristics, and
these characteristics could be expressed using the small-scale
turbulent components.