Utilization of Sugarcane Bagasses for Lactic Acid Production by acid Hydrolysis and Fermentation using Lactobacillus sp
Sugarcane bagasses are one of the most extensively used agricultural residues. Using acid hydrolysis and fermentation, conversion of sugarcane bagasses to lactic acid was technically and economically feasible. This research was concerned with the solubility of lignin in ammonium hydroxide, acid hydrolysis and lactic acid fermentation by Lactococcus lactis, Lactobacillus delbrueckii, Lactobacillus plantarum, and Lactobacillus casei. The lignin extraction results for different ammonium hydroxide concentrations showed that 10 % (v/v) NH4OH was favorable to lignin dissolution. Acid hydrolysis can be enhanced with increasing acid concentration and reaction temperature. The optimum glucose and xylose concentrations occurred at 121 ○C for 1 hour hydrolysis time in 10% sulphuric acid solution were 32 and 11 g/l, respectively. In order to investigate the significance of medium composition on lactic acid production, experiments were undertaken whereby a culture of Lactococcus lactis was grown under various glucose, peptone, yeast extract and xylose concentrations. The optimum medium was composed of 5 g/l glucose, 2.5 g/l xylose, 10 g/l peptone and 5 g/l yeast extract. Lactococcus lactis represents the most efficient for lactic acid production amongst those considered. The lactic acid fermentation by Lactococcus lactis after 72 hours gave the highest yield of 1.4 (g lactic acid per g reducing sugar).
[1] Young-Jung Wee, Jin-Nam Kim, Jong-Sun Yun, and Hwa-Won Ryu.
(2004) "Utilization of sugar molasses for economical l(+)-lactic acid
productionby batch fermentation of Enterococcus faecalis", Enzyme and
Microbial Technology 35, 568-573.
[2] Rojan P. John, G.S. Anisha, K. Madhavan Nampoothiri, and Ashok
Pandey. (2009) "Direct lactic acid fermentation: Focus on simultaneous
saccharification and lactic acid production", Biotechnology Advances
27, 145-152.
[3] Limin Wang, Bo Zhao, Bo Liu, Chunyu Yang, Bo Yu, Qinggang Li,
Cuiqing Ma, Ping Xu, and Yanhe Ma. (2010) "Efficient production of Llactic
from cassava powder by Lactobacillus rhamnosus", Bioresource
Technology 101, 7895-7901.
[4] J.M. Herna'ndez-Salas, M.S. Villa-Rami'rez, J.S. Veloz-Rendo'n, K.N.
Rivera-Herna'ndez, R.A. Gonza'lez-Ce'sar, M.A. Plascencia-Espinosa,
and S.R. Trejo-Estrada. (2009) "Comparative hydrolysis and
fermentation of sugarcane and agave bagasse", Bioresource Technology
100, 1238-1245.
[5] Pattana Laopaiboon, Arthit Thani, Vichean Leelavatcharamas, and
Lakkana Laopaiboon (2010) "Acid hydrolysis of sugarcane bagasse for
lactic acid production", Bioresource Technology 101, 1036-1043.
[6] Gopal Reddy, Md. Altaf, B.J. Naveena, M. Venkateshwar, E. Vijay
Kumar (2008) "Amylolytic bacterial lactic acid fermentation",
Biotechnology Advances 26, 22-34.
[7] Arlington. (1990) "AOAC. Official Method of Analysis", VA:
Association of Official Analytical Chemists. 15thed.
[8] G.L. Miller. (1959) "Analytical Chemistry use of Di-nitro salicylic Acid
Regent for Determintion of Reducing Sugar", 31, 426-428.
[9] Tuovinen, O.H. (1990) "Biological fundaments of minerals of mineral
leaching processes", in Ehrlich, H. L. and Brierley, C. L. (eds) Microbial
Mineral Recovery. New York: Mc Graw-Hill.
[10] Liliana Serna Cock and Aida Rodriguez de Stouvenel (2006)
"Lactic acid production by a strain of Lactococcus lactis subs lactis
isolated from sugar cane plants", Journal of Biotechnology 9, 40-45.
[11] Rojan P. John, Rajeev K. Sukumaran, K. Madhavan Nampoothiri and
Ashok Pandey (2007) "Statistical optimization of simultaneous
saccharification and L(+)- lactic acid fermentation from cassava bagasse
using mixed culture of lactobacilli by response surface methodology",
Biochemical Engineering Journal 36, 262-267.
[12] Tiina Michelson, Karin Kask, Eerik Jogi, Ene Talpsep, Indrek Suitso
and Allan Nurk (2006) "L(+)-Lactic acid producer Bacillus coagulans
SIM-7 DSM 14043 and its comparison with Lactobacillus delbrueckii
ssp. Lactis DSM 20073", Enzyme and Microbial Technology 39, 861-
867.
[13] Sachin R. Kadam, Sudarsham S. Patil, Kulbhushan B. Bastawde, Jayant
M. Khire and Digambar V. Gokhale (2006) "Strain improvement of
Lactobacillus delbrueckii NCIM 2365 for lactic acid production",
Process Biochemistry 41, 120-126.
[14] Hiroyuki Honda, Yoshio Toyama, Hiroshi Takahashi, Takuo Nakazeko
and Takeshi Kobayashi (1995) "Effective lactic acid production by twostage
extractive fermentation", Journal of fermentation and
bioengineering 79, 589-593.
[15] Hassan K. Sreenath, Ana B. Moldes, Richard G. Koegel and Richard J.
Straub (2001) "Lactic acid production by simultaneous saccharification
and fermentation of alfalfa fiber", Journal of bioscience and
bioengineering 92, 518-523.
[16] E. Papamanoli, N. Tzanetakis, E. Litopoulou-Tzanetaki, and P.
Kotzekidou (2003) "Characterization of lactic acid isolated from a Greek
dry-fermented sausage in respect of their technological and probiotic
properties", Meat Science 65, 859-867
[17] Inayara C.A. Lacerda, Rose L. Miranda, Beatriz M. Borelli, Álvaro C.
Nunes, Regina M.D. Nardi, Marc-André Lachance, Caelos A. Rosa
(2005) "Lactic acid bacteria and yeasts associated with spontaneous
fermentations during the production of sour cassava starch in Brazil",
International Journal of food Microbiology 105, 213-219.
[18] Shaofeng Ding and Tianwei Tan (2006) "L-lactic acid production by
Lactobaciilus casei fermentation using different fed-batch feeding
strategies", Process Biochemistry 41, 1451-1454. G. O. Young,
"Synthetic structure of industrial plastics (Book style with paper title and
editor)," in Plastics, 2nd ed. vol. 3, J. Peters, Ed. New York: McGraw-
Hill, 1964, pp. 15-64.
[1] Young-Jung Wee, Jin-Nam Kim, Jong-Sun Yun, and Hwa-Won Ryu.
(2004) "Utilization of sugar molasses for economical l(+)-lactic acid
productionby batch fermentation of Enterococcus faecalis", Enzyme and
Microbial Technology 35, 568-573.
[2] Rojan P. John, G.S. Anisha, K. Madhavan Nampoothiri, and Ashok
Pandey. (2009) "Direct lactic acid fermentation: Focus on simultaneous
saccharification and lactic acid production", Biotechnology Advances
27, 145-152.
[3] Limin Wang, Bo Zhao, Bo Liu, Chunyu Yang, Bo Yu, Qinggang Li,
Cuiqing Ma, Ping Xu, and Yanhe Ma. (2010) "Efficient production of Llactic
from cassava powder by Lactobacillus rhamnosus", Bioresource
Technology 101, 7895-7901.
[4] J.M. Herna'ndez-Salas, M.S. Villa-Rami'rez, J.S. Veloz-Rendo'n, K.N.
Rivera-Herna'ndez, R.A. Gonza'lez-Ce'sar, M.A. Plascencia-Espinosa,
and S.R. Trejo-Estrada. (2009) "Comparative hydrolysis and
fermentation of sugarcane and agave bagasse", Bioresource Technology
100, 1238-1245.
[5] Pattana Laopaiboon, Arthit Thani, Vichean Leelavatcharamas, and
Lakkana Laopaiboon (2010) "Acid hydrolysis of sugarcane bagasse for
lactic acid production", Bioresource Technology 101, 1036-1043.
[6] Gopal Reddy, Md. Altaf, B.J. Naveena, M. Venkateshwar, E. Vijay
Kumar (2008) "Amylolytic bacterial lactic acid fermentation",
Biotechnology Advances 26, 22-34.
[7] Arlington. (1990) "AOAC. Official Method of Analysis", VA:
Association of Official Analytical Chemists. 15thed.
[8] G.L. Miller. (1959) "Analytical Chemistry use of Di-nitro salicylic Acid
Regent for Determintion of Reducing Sugar", 31, 426-428.
[9] Tuovinen, O.H. (1990) "Biological fundaments of minerals of mineral
leaching processes", in Ehrlich, H. L. and Brierley, C. L. (eds) Microbial
Mineral Recovery. New York: Mc Graw-Hill.
[10] Liliana Serna Cock and Aida Rodriguez de Stouvenel (2006)
"Lactic acid production by a strain of Lactococcus lactis subs lactis
isolated from sugar cane plants", Journal of Biotechnology 9, 40-45.
[11] Rojan P. John, Rajeev K. Sukumaran, K. Madhavan Nampoothiri and
Ashok Pandey (2007) "Statistical optimization of simultaneous
saccharification and L(+)- lactic acid fermentation from cassava bagasse
using mixed culture of lactobacilli by response surface methodology",
Biochemical Engineering Journal 36, 262-267.
[12] Tiina Michelson, Karin Kask, Eerik Jogi, Ene Talpsep, Indrek Suitso
and Allan Nurk (2006) "L(+)-Lactic acid producer Bacillus coagulans
SIM-7 DSM 14043 and its comparison with Lactobacillus delbrueckii
ssp. Lactis DSM 20073", Enzyme and Microbial Technology 39, 861-
867.
[13] Sachin R. Kadam, Sudarsham S. Patil, Kulbhushan B. Bastawde, Jayant
M. Khire and Digambar V. Gokhale (2006) "Strain improvement of
Lactobacillus delbrueckii NCIM 2365 for lactic acid production",
Process Biochemistry 41, 120-126.
[14] Hiroyuki Honda, Yoshio Toyama, Hiroshi Takahashi, Takuo Nakazeko
and Takeshi Kobayashi (1995) "Effective lactic acid production by twostage
extractive fermentation", Journal of fermentation and
bioengineering 79, 589-593.
[15] Hassan K. Sreenath, Ana B. Moldes, Richard G. Koegel and Richard J.
Straub (2001) "Lactic acid production by simultaneous saccharification
and fermentation of alfalfa fiber", Journal of bioscience and
bioengineering 92, 518-523.
[16] E. Papamanoli, N. Tzanetakis, E. Litopoulou-Tzanetaki, and P.
Kotzekidou (2003) "Characterization of lactic acid isolated from a Greek
dry-fermented sausage in respect of their technological and probiotic
properties", Meat Science 65, 859-867
[17] Inayara C.A. Lacerda, Rose L. Miranda, Beatriz M. Borelli, Álvaro C.
Nunes, Regina M.D. Nardi, Marc-André Lachance, Caelos A. Rosa
(2005) "Lactic acid bacteria and yeasts associated with spontaneous
fermentations during the production of sour cassava starch in Brazil",
International Journal of food Microbiology 105, 213-219.
[18] Shaofeng Ding and Tianwei Tan (2006) "L-lactic acid production by
Lactobaciilus casei fermentation using different fed-batch feeding
strategies", Process Biochemistry 41, 1451-1454. G. O. Young,
"Synthetic structure of industrial plastics (Book style with paper title and
editor)," in Plastics, 2nd ed. vol. 3, J. Peters, Ed. New York: McGraw-
Hill, 1964, pp. 15-64.
@article{"International Journal of Chemical, Materials and Biomolecular Sciences:60197", author = "Woranart Jonglertjunya and Nattawadee Pranrawang and Nuanyai Phookongka and Thanasak Sridangtip and Watthana Sawedrungreang and Chularat Krongtaew", title = "Utilization of Sugarcane Bagasses for Lactic Acid Production by acid Hydrolysis and Fermentation using Lactobacillus sp", abstract = "Sugarcane bagasses are one of the most extensively used agricultural residues. Using acid hydrolysis and fermentation, conversion of sugarcane bagasses to lactic acid was technically and economically feasible. This research was concerned with the solubility of lignin in ammonium hydroxide, acid hydrolysis and lactic acid fermentation by Lactococcus lactis, Lactobacillus delbrueckii, Lactobacillus plantarum, and Lactobacillus casei. The lignin extraction results for different ammonium hydroxide concentrations showed that 10 % (v/v) NH4OH was favorable to lignin dissolution. Acid hydrolysis can be enhanced with increasing acid concentration and reaction temperature. The optimum glucose and xylose concentrations occurred at 121 ○C for 1 hour hydrolysis time in 10% sulphuric acid solution were 32 and 11 g/l, respectively. In order to investigate the significance of medium composition on lactic acid production, experiments were undertaken whereby a culture of Lactococcus lactis was grown under various glucose, peptone, yeast extract and xylose concentrations. The optimum medium was composed of 5 g/l glucose, 2.5 g/l xylose, 10 g/l peptone and 5 g/l yeast extract. Lactococcus lactis represents the most efficient for lactic acid production amongst those considered. The lactic acid fermentation by Lactococcus lactis after 72 hours gave the highest yield of 1.4 (g lactic acid per g reducing sugar).
", keywords = "sugarcane bagasses, acid hydrolysis, lactic acid,
fermentation", volume = "6", number = "6", pages = "510-6", }
