The Expression of Lipoprotein Lipase Gene with Fat Accumulations and Serum Biochemical Levels in Betong (KU Line) and Broiler Chickens

Betong chicken is a slow growing and a lean strain of chicken, while the rapid growth of broiler is accompanied by increased fat. We investigated the growth performance, fat accumulations, lipid serum biochemical levels and lipoprotein lipase (LPL) gene expression of female Betong (KU line) at the age of 4 and 6 weeks. A total of 80 female Betong chickens (KU line) and 80 female broiler chickens were reared under open system (each group had 4 replicates of 20 chicks per pen). The results showed that feed intake and average daily gain (ADG) of broiler chicken were significantly higher than Betong (KU line) (P < 0.01), while feed conversion ratio (FCR) of Betong (KU line) at week 6 were significantly lower than broiler chicken (P < 0.01) at 6 weeks. At 4 and 6 weeks, two birds per replicate were randomly selected and slaughtered. Carcass weight did not significantly differ between treatments; the percentage of abdominal fat and subcutaneous fat yield was higher in the broiler (P < 0.01) at 4 and 6 week. Total cholesterol and LDL level of broiler were higher than Betong (KU line) at 4 and 6 weeks (P < 0.05). Abdominal fat samples were collected for total RNA extraction. The cDNA was amplified using primers specific for LPL gene expression and analysed using real-time PCR. The results showed that the expression of LPL gene was not different when compared between Betong (KU line) and broiler chickens at the age of 4 and 6 weeks (P > 0.05). Our results indicated that broiler chickens had high growth rate and fat accumulation when compared with Betong (KU line) chickens, whereas LPL gene expression did not differ between breeds.

• W. Loongyai
• N. Saengsawang
• W. Danvilai
• C. Kridtayopas
• P. Sopannarath
• C. Bunchasak

• Lipoprotein lipase gene
• Betong (KU line)
• broiler
• abdominal fat
• gene expression.

[1] T. V. Nguyen, and C Bunchasak, “Effect of dietary protein and energy on growth performance and carcass characteristics of Betong chicken at early growth stage,” Songklanakarin J. Sci. Technol, vol. 27, no. 6, pp. 1171-1178, Feb. 2005.
[2] A. Putsakul, C. Bunchasak, B. Chomtee, S. Kao-ian, and P. Sopannarath, “Effect of dietary protein and metabolizable energy levels on growth and carcass yields,” in 48th Kasetsart University Annu. Conf. Animal Vaterinary Medicine, Bangkok, Thailand, 2010, pp. 158-166.
[3] H. D. Griffin, and D. Hermier, “Plasma lipoprotein metabolism and fattening on poultry,” in Leanness in Domestic Birds, B. Leclercq, and C. C. Whitehead ed. Butterworths, London, 1988, pp. 175–201
[4] D. G. Harry, G. Kunda, W. Dawn, and C. Simon, “Adipose tissue lipogenesis and fat deposition in leaner broiler chickens,” J. Nutr., vol. 122, no. 2, pp. 363–368, Feb. 1992.
[5] A. Bensadoun, “Lipoprotein lipase,” Annu. Rev. Nutr., vol. 11, pp. 217–237, Jul. 1991.
[6] K. Sato, Y. Akiba, Y. Chida, and K. Takahashi, “Lipoprotein hydrolysis and fat accumulation in chicken adipose tissues are reduced by chronic administration of lipoprotein lipase monoclonal antibodies,” Poult. Sci., vol. 78, no. 9, pp. 1286–1291, Sep. 1999.
[7] T. V. Nguyen, C. Bunchasak, and S, Chantsavang, “Effects of dietary protein and energy on growth performance and carcass characteristics of betong chickens (Gallus domesticus) during growing period,” Int. J. Poult. Sci., vol. 9, no. 5, pp. 468-472, 2010.
[8] E. Tůmová, and A.Teimouri, “Fat deposition in the broiler chickens,” Sci. Agric. Bohem., vol. 41, no. 2, pp. 120-128, Feb. 2010.
[9] M. Amal, L. Michel, G. Solang, and K. Maryline, “Effect of dietary fats on hepatic lipid metabolism in the growing turkey,” Comp. Biochem. Physiol., Part B, vol. 132, no. 2, pp. 473–483, Jun. 2002.
[10] N. Deeb, and S. J. Lamont, “Genetic Architecture of Growth and Body Composition in Unique Chicken Populations,” J. Hered., vol. 93, no. 2, pp. 107-118, Mar. 2002.
[11] H. D. Griffin, and C. C. Whitehead, “Identification of lean and fat turkeys by measurement of plasma very low density lipoprotein concentration,” Br Poult Sci., vol. 26, no. 1, pp. 51-56, Nov. 1983.
[12] C. C. Whitehead, and H. Griffin, “Plasma lipoprotein concentration as an indicator of fatness in broilers: effect of age and diet,” Br. Poult. Sci., vol. 23, no. 4, pp. 299–305, Jul. 1982.
[13] H. D. Griffin, S. C. Butterwith, and C. Goddard, “Contribution of lipoprotein lipase to differences in fatness between broiler and layer-strain chickens,” Br.Poult. Sci., vol. 28, no. 2, pp. 197–206. Jun. 1987.
[14] R. W. Rosebrough, B. A. Russell, S. M. Poch, and M. P. Richards. “Expression of lipogenic enzymes in chickens,” Comp. Biochem. Physiol. A. Mol. Integr. Physiol., vol. 147, no. 1, pp. 215–222, May. 2007.
[15] R. W. Rosebrough, and N. C. Steele, “Energy and protein relations in the broiler. 1. Effect of protein levels and feeding regimes on growth, body composition and in vitro lipogenesis in broiler chicks,” Poult. Sci., vol. 64, no. 1, pp. 119–129, Jan. 1985.
[16] R. W. Rosebrough, and N. C. Steele, “Protein and energy relations in the broiler 3. Growth and in vitro metabolism in male and female chickens used as parent stock,” Growth., vol. 49, no. 1, pp. 63–75, Spring. 1985.
[17] R. W. Rosebrough, A. D. Mitchell, M. F. Von Vleck, N. C. Steele, “Protein and energy relations in the broiler chicken. 8. Comparisons involving protein- and lysine-adequate and inadequate diets on lipid metabolism,” Br. J. Nutr., vol. 64, no. 2, pp. 515–523, Sep. 1990.
[18] C. F. Semenkovich, T. Coleman, and R. Goforth, “Physiologic concentrations of glucose regulate fatty acid synthase activity in HepG2 cells by mediating fatty acid synthase mRNA stability,” J. Biol. Chem., vol. 268, no. 10, pp. 6961–6970, Apr. 1993.