Swelling Behavior and Cytotoxicity of Maleic Acid Grafted Chitosan

Chitosan is an attractive polysaccharide obtained by deacetylation of an abundant natural biopolymer called chitin. Chitin and chitosan are excellent materials. To improve the potential of chitin and chitosan modification is needed. In the present study, grafting of maleic acid on to chitosan by cerium ammonium nitrate in acetic acid solution was investigated with use of a microwave and reflux system. The grafted chitosan was characterized by using a Fourier-transform infrared spectrometry. The solubility and swelling behavior of grafted chitosans were determined in acetate buffer (pH 3.6), citrophosphate buffer (pH 5.6 and pH 7.0), and boric buffer (pH 9.2) solutions. The sample obtained by microwave system with use of a chitosan/maleic anhydride/ceric ammonium nitrate 0.2/3.922/0.99 gram of raw material within 30 minute showed the maximum swelling ratio (13.6) in boric buffer solution.

Authors:

• Sevil Yucel
• Zafer Omer Ozdemir
• Cem Kesgin
• Pinar Terzioglu
• Simten Unlu
• Yagmur Erdogan
• Kubra Pusat

Keywords:

• Chitosan
• cytotoxicity
• grafted
• maleic acid
• swell.

References:

[1] M.N.V. R. Kumar, " A review of chitin and chitosan applications,"
Reactive & Functional Polymers , vol. 46 ,2000,pp. 1-27.
[2] R. Jayakumar , M. Prabaharan, S.V. Nair,and H. Tamura, " Novel chitin
and chitosan nanofibers in biomedical applications,"Biotechnology
Advances , vol.28 ,2010,pp. 142-150.
[3] S. Tanodekaew, M.Prasitsilp, S. Swasdison, B. Thavornyutikarn, T.
Pothsree,and R. Pateepasen, " Preparation of acrylic grafted chitin for
wound dressing application," Biomaterials ,vol.25 ,2004, pp. 1453-
1460.
[4] G.R. Mahdavinia, A. Pourjavadi , H. Hosseinzadeh,and M.J.
Zohuriaan, " Modified chitosan 4. Superabsorbent hydrogels from
poly(acrylic acid-co-acrylamide) grafted chitosan with salt- and pHresponsiveness
properties,"European Polymer Journal ,vol. 40 ,2004,pp.
1399-1407.
[5] P. A. Sandford, " In Chitin and Chitosan," eds. G. Skjak-Braek, T.
Anthonsen, and P. Sandford. London: Elsevier Applied Science, 1989,
pp. 51-69.
[6] W. Xie, P. Xu, and Qing Liu, " Antioxidant Activity of Water-Soluble
Chitosan Derivatives," Bioorganic & MedicinalChemistry Letters ,vol.
11, 2001, pp. 1699-1701.
[7] H. Sashiwa, and S.Aiba, " Chemically modified chitin and chitosan as
biomaterials," Prog. Polym. Sci. ,vol. 29 ,2004, pp. 887-908.
[8] H. N. Hasipoglu, E. Yilmaz, O. Yilmaz,and H. Caner, " Preparation and
Characterization of Maleic Acid Grafted Chitosan," International
Journal of Polymer Anal. Charact., vol. 10, 2005, pp. 313-327.
[9] H. Caner, H. Hasipoglu, O. Yilmaz, and E. Yilmaz, " Graft
copolymerization of 4-vinylpyridine on to chitosan-1. By ceric ion
initiation," Eur. Polym. J. , vol. 34, 1998, pp. 493-497.
[10] A. Lagos, and J. Reyes, "Grafting onto chitosan. 1: Graft
copolymerization of methyl methacrylate onto chitosan with Fenton-s
reagent (Fe+2 -H2O2) ," J. Polym. Sci. Part A. Polym. Chem. ,vol. 26,
1988, pp. 985-991.
[11] A. Neamnark, N. Sanchavanakit, P. Pavasant, T. Bunaprasert, P.
Supaphol, R. Rujiravanit, "In vitro biocompatibility evaluations of
hexanoyl chitosan film," Carbohydrate Polymers, vol. 68, 2007, 166-
172.