Simultaneous Saccharification and Fermentation(SSF) of Sugarcane Bagasse - Kinetics and Modeling
Simultaneous Saccharification and Fermentation (SSF) of sugarcane bagasse by cellulase and Pachysolen tannophilus MTCC *1077 were investigated in the present study. Important process variables for ethanol production form pretreated bagasse were optimized using Response Surface Methodology (RSM) based on central composite design (CCD) experiments. A 23 five level CCD experiments with central and axial points was used to develop a statistical model for the optimization of process variables such as incubation temperature (25–45°) X1, pH (5.0–7.0) X2 and fermentation time (24–120 h) X3. Data obtained from RSM on ethanol production were subjected to the analysis of variance (ANOVA) and analyzed using a second order polynomial equation and contour plots were used to study the interactions among three relevant variables of the fermentation process. The fermentation experiments were carried out using an online monitored modular fermenter 2L capacity. The processing parameters setup for reaching a maximum response for ethanol production was obtained when applying the optimum values for temperature (32°C), pH (5.6) and fermentation time (110 h). Maximum ethanol concentration (3.36 g/l) was obtained from 50 g/l pretreated sugarcane bagasse at the optimized process conditions in aerobic batch fermentation. Kinetic models such as Monod, Modified Logistic model, Modified Logistic incorporated Leudeking – Piret model and Modified Logistic incorporated Modified Leudeking – Piret model have been evaluated and the constants were predicted.
[1] G J. Zaldivar, J. Nielsen, and L.Olsson, "Fuel ethanol production
from lignocellulose: A challenge for metabolic engineering and
process integration", Appl. Microbiol. Biotechnol., vol. 56, pp. 17 -
34, 2001.
[2] M. Karuppaiya, E. Sasikumar, T. Viruthagiri, and V.Vijayagopal,
"Optimization of process parameters using Response Surface
Methodology (RSM) for ethanol production from waste cashew apple
juice using Zymomonas mobilis", Chem. Eng. Comm. vol.196, pp. 1-
11, 2009.
[3] J.D.Wright, "Ethanol from biomass by enzymatic hydrolysis", Chem.
Eng. Prog., vol. 84, no.8, pp.62 -74, 1988.
[4] L.Olsson, and B.Hahn-Hagerdal, "Fermentation of lignocellulosic
hydrolysates for ethanol production", Enzym. Microb. Technol.,
vol.18, pp.312 - 33, 1996.
[5] A. Pandey, C.R. Soccol, P. Nigam, and V.T. Soccol,
"Biotechnological potential of agro-industrial residues I: sugarcane
bagasse", Biores. Technol., vol.74, pp. 69 - 81, 2000.
[6] F. Haagensen, and B.K.Ahring, "Enzymatic hydrolysis and glucose
fermentation of wet oxidized sugarcane bagasse and rice straw for
bioethanol production", Riso-R-1517 (EN), vol. 1, pp.184 -195, 2002.
[7] L.Dawson, and R. Boopathy, "Use of post-harvest sugarcane residue
for ethanol production", Biores. Technol., vol. 98, pp.1695 - 1699,
2007.
[8] Ye Sun, and J. Cheng, "Hydrolysis of lignocellulosic materials for
ethanol production: A review", Biores. Technol., vol. 83, pp.1 - 11,
2002.
[9] M.G. Adsul, J.E.Ghule, H.Shaikh, R.Singh, K.B.Bastawde,
D.V.Gokhale, and A.J. Varma, "Enzymatic hydrolysis of delignified
bagasse polysaccharides", Carbohy. Polym., vol.62, pp. 6 - 10, 2005.
[10] P. Ghosh, N.B.Pamment, and W.R.B.Martin, "Simultaneous
saccharification and fermentation of cellulose: effect of β-Dglucosidase
activity and ethanol inhibition of cellulases", Enzym.
Microb. Technol., vol. 4, pp. 425 - 430, 1982.
[11] C.E.Wyman, "Ethanol from lignocellulosic biomass: Technology,
economics, and opportunities", Biores. Technol., vol. 50, pp.3 - 16,
1994.
[12] R. Balusu, R.R.Paduru, S.K.Kuravi, G.Seenaya, and G.Reddy,
"Optimization of critical medium components using response surface
methodology for ethanol production from cellulosic biomass by
Clostridium thermocellum SS19", Proc. Biochem., vol. 40, pp. 3025 -
3030, 2005.
[13] O.Jargalsaikhan, and N. Saracoglu, "Application of experimental
design method for ethanol production by fermentation of sunflower
seed hull hydrolysate using Pichia stipitis NRRL-124", Chem. Eng.
Comm., vol.196, pp.93 - 103, 2009.
[14] D. M. Updegroff, "Estimation of cellulose by anthrone reagent",
Anal. Chem., vol.32, pp. 420 - 423, 1969.
[15] M. Mandel, R. Andreotti, and C. Roche, "Measurement of
saccharifying cellulose", Biotechnol. Bioeng. Symp., vol. 6, pp.21 -
33, 1976.
[16] J. Szczodrak, and J. Fiedurek, "Technology for conversion of
lignocellulosic biomass to ethanol", Biomass. Bioenerg., vol.10,
pp.367 - 375, 1996.
[17] W.E.Kaar, C.V.Gutierrez, and C.M.Kinoshita, "Steam explosion of
sugarcane bagasse as a pretreatment for conversion to ethanol",
Biomass. Bioenerg., vol.14, no.3, pp.277 - 287, 1998.
[18] M.Taniguchi, M.Tanaka, R. Matsuno, and T. Kamikubo, "Evaluation
of chemical pretreatment for enzymatic solubilization of rice straw",
European J. Appl. Microbiol. Biotechnol. 14: 35 - 39
[19] G.L.Miller, "Use of dinitrosalicylic acid reagent for determination of
total reducing sugar", Anal. Chem., vol.31, pp. 420 - 426, 1959.
[20] M.Giovanni, "Response surface methodology and product
optimization", J. Food Technol. vol. 37, pp. 41- 45, 1983.
[21] M.F.Anjum, I.Tasadduq, and K. Al-Sultan, "Response surface
methodology: A neural network approach", European J. Operat. Res.,
vol.101, pp.65-73, 1997.
[22] K. Hornik, M. Stinchcombe, and H. White, "Multilayer feed forward
networks are universal approximators", Neural Net., vol.2, pp.359 -
366, 1989.
[23] R. Dhanasekar, T. Viruthagiri, and P.L. Sabarathinam, "Poly(3-
hydroxy butyrate) synthesis from a mutant strain Azotobacter
vinelandii utilizing glucose in a batch reactor", Biochem. Eng. J., vol.
16, no.1, pp.1 - 8, 2003.
[24] E. Sasikumar, and T. Viruthagiri, "Optimization of process conditions
using response surface methodology (RSM) for ethanol production
from pretreated sugarcane bagasse: Kinetics and modeling",
Bioenerg. Res. vol.1, pp. 239 - 247, 2008.
[1] G J. Zaldivar, J. Nielsen, and L.Olsson, "Fuel ethanol production
from lignocellulose: A challenge for metabolic engineering and
process integration", Appl. Microbiol. Biotechnol., vol. 56, pp. 17 -
34, 2001.
[2] M. Karuppaiya, E. Sasikumar, T. Viruthagiri, and V.Vijayagopal,
"Optimization of process parameters using Response Surface
Methodology (RSM) for ethanol production from waste cashew apple
juice using Zymomonas mobilis", Chem. Eng. Comm. vol.196, pp. 1-
11, 2009.
[3] J.D.Wright, "Ethanol from biomass by enzymatic hydrolysis", Chem.
Eng. Prog., vol. 84, no.8, pp.62 -74, 1988.
[4] L.Olsson, and B.Hahn-Hagerdal, "Fermentation of lignocellulosic
hydrolysates for ethanol production", Enzym. Microb. Technol.,
vol.18, pp.312 - 33, 1996.
[5] A. Pandey, C.R. Soccol, P. Nigam, and V.T. Soccol,
"Biotechnological potential of agro-industrial residues I: sugarcane
bagasse", Biores. Technol., vol.74, pp. 69 - 81, 2000.
[6] F. Haagensen, and B.K.Ahring, "Enzymatic hydrolysis and glucose
fermentation of wet oxidized sugarcane bagasse and rice straw for
bioethanol production", Riso-R-1517 (EN), vol. 1, pp.184 -195, 2002.
[7] L.Dawson, and R. Boopathy, "Use of post-harvest sugarcane residue
for ethanol production", Biores. Technol., vol. 98, pp.1695 - 1699,
2007.
[8] Ye Sun, and J. Cheng, "Hydrolysis of lignocellulosic materials for
ethanol production: A review", Biores. Technol., vol. 83, pp.1 - 11,
2002.
[9] M.G. Adsul, J.E.Ghule, H.Shaikh, R.Singh, K.B.Bastawde,
D.V.Gokhale, and A.J. Varma, "Enzymatic hydrolysis of delignified
bagasse polysaccharides", Carbohy. Polym., vol.62, pp. 6 - 10, 2005.
[10] P. Ghosh, N.B.Pamment, and W.R.B.Martin, "Simultaneous
saccharification and fermentation of cellulose: effect of β-Dglucosidase
activity and ethanol inhibition of cellulases", Enzym.
Microb. Technol., vol. 4, pp. 425 - 430, 1982.
[11] C.E.Wyman, "Ethanol from lignocellulosic biomass: Technology,
economics, and opportunities", Biores. Technol., vol. 50, pp.3 - 16,
1994.
[12] R. Balusu, R.R.Paduru, S.K.Kuravi, G.Seenaya, and G.Reddy,
"Optimization of critical medium components using response surface
methodology for ethanol production from cellulosic biomass by
Clostridium thermocellum SS19", Proc. Biochem., vol. 40, pp. 3025 -
3030, 2005.
[13] O.Jargalsaikhan, and N. Saracoglu, "Application of experimental
design method for ethanol production by fermentation of sunflower
seed hull hydrolysate using Pichia stipitis NRRL-124", Chem. Eng.
Comm., vol.196, pp.93 - 103, 2009.
[14] D. M. Updegroff, "Estimation of cellulose by anthrone reagent",
Anal. Chem., vol.32, pp. 420 - 423, 1969.
[15] M. Mandel, R. Andreotti, and C. Roche, "Measurement of
saccharifying cellulose", Biotechnol. Bioeng. Symp., vol. 6, pp.21 -
33, 1976.
[16] J. Szczodrak, and J. Fiedurek, "Technology for conversion of
lignocellulosic biomass to ethanol", Biomass. Bioenerg., vol.10,
pp.367 - 375, 1996.
[17] W.E.Kaar, C.V.Gutierrez, and C.M.Kinoshita, "Steam explosion of
sugarcane bagasse as a pretreatment for conversion to ethanol",
Biomass. Bioenerg., vol.14, no.3, pp.277 - 287, 1998.
[18] M.Taniguchi, M.Tanaka, R. Matsuno, and T. Kamikubo, "Evaluation
of chemical pretreatment for enzymatic solubilization of rice straw",
European J. Appl. Microbiol. Biotechnol. 14: 35 - 39
[19] G.L.Miller, "Use of dinitrosalicylic acid reagent for determination of
total reducing sugar", Anal. Chem., vol.31, pp. 420 - 426, 1959.
[20] M.Giovanni, "Response surface methodology and product
optimization", J. Food Technol. vol. 37, pp. 41- 45, 1983.
[21] M.F.Anjum, I.Tasadduq, and K. Al-Sultan, "Response surface
methodology: A neural network approach", European J. Operat. Res.,
vol.101, pp.65-73, 1997.
[22] K. Hornik, M. Stinchcombe, and H. White, "Multilayer feed forward
networks are universal approximators", Neural Net., vol.2, pp.359 -
366, 1989.
[23] R. Dhanasekar, T. Viruthagiri, and P.L. Sabarathinam, "Poly(3-
hydroxy butyrate) synthesis from a mutant strain Azotobacter
vinelandii utilizing glucose in a batch reactor", Biochem. Eng. J., vol.
16, no.1, pp.1 - 8, 2003.
[24] E. Sasikumar, and T. Viruthagiri, "Optimization of process conditions
using response surface methodology (RSM) for ethanol production
from pretreated sugarcane bagasse: Kinetics and modeling",
Bioenerg. Res. vol.1, pp. 239 - 247, 2008.
@article{"International Journal of Chemical, Materials and Biomolecular Sciences:57913", author = "E.Sasikumar and T.Viruthagiri", title = "Simultaneous Saccharification and Fermentation(SSF) of Sugarcane Bagasse - Kinetics and Modeling", abstract = "Simultaneous Saccharification and Fermentation (SSF) of sugarcane bagasse by cellulase and Pachysolen tannophilus MTCC *1077 were investigated in the present study. Important process variables for ethanol production form pretreated bagasse were optimized using Response Surface Methodology (RSM) based on central composite design (CCD) experiments. A 23 five level CCD experiments with central and axial points was used to develop a statistical model for the optimization of process variables such as incubation temperature (25–45°) X1, pH (5.0–7.0) X2 and fermentation time (24–120 h) X3. Data obtained from RSM on ethanol production were subjected to the analysis of variance (ANOVA) and analyzed using a second order polynomial equation and contour plots were used to study the interactions among three relevant variables of the fermentation process. The fermentation experiments were carried out using an online monitored modular fermenter 2L capacity. The processing parameters setup for reaching a maximum response for ethanol production was obtained when applying the optimum values for temperature (32°C), pH (5.6) and fermentation time (110 h). Maximum ethanol concentration (3.36 g/l) was obtained from 50 g/l pretreated sugarcane bagasse at the optimized process conditions in aerobic batch fermentation. Kinetic models such as Monod, Modified Logistic model, Modified Logistic incorporated Leudeking – Piret model and Modified Logistic incorporated Modified Leudeking – Piret model have been evaluated and the constants were predicted.
", keywords = "Sugarcane bagasse, ethanol, optimization,Pachysolen tannophilus.", volume = "4", number = "1", pages = "85-8", }
