Image mosaicing techniques are usually employed to offer researchers a wider field of view of microscopic image of biological samples. a mosaic is commonly achieved using automated microscopes and often with one “color" channel, whether it refers to natural or fluorescent analysis. In this work we present a method to achieve three subsequent mosaics of the same part of a stem cell culture analyzed in phase contrast and in fluorescence, with a common non-automated inverted microscope. The mosaics obtained are then merged together to mark, in the original contrast phase images, nuclei and cytoplasm of the cells referring to a mosaic of the culture, rather than to single images. The experiments carried out prove the effectiveness of our approach with cultures of cells stained with calcein (green/cytoplasm and nuclei) and hoechst (blue/nuclei) probes.
[1] I. Tognarini, S. Sorace, R. Zonefrati, G. Galli, A. Gozzini, S. C. Sala,
G. Thyrion, A. Carossino, A. Tanini, C. Mavilia, C. Azzari, F. Sbaiz,
A. Facchini, R. Capanna, and M. Brandi, "In vitro differentiation of
human mesenchymal stem cells on ti6al4v surfaces," Biomaterials,
vol. 29, pp. 809-824, 2008.
[2] K. S. Fubito Toyama and J. Miyamichi, "Image mosaicing from a set of
images without configuration information," in Proceedings of the 17th
International Conference on Pattern Recognition (ICPRŠ04), Cambridge
UK, August 23, vol. 2, 2004, pp. 899-902.
[3] K. Loewke, D. Camarillo, W. Piyawattanametha, D. Breeden, and
K. Salisbury, "Real-time image mosaicing with a hand-held dual-axes
confocal microscope," in Proceedings of the SPIE Conf. on Endoscopic
Microscopy III, San Jose, CA, USA, January 20, vol. 6851, 2008, pp.
1-9.
[4] Y. Pang, A. A. Ucuzian, A. Matsumura, E. M. Brey, A. A. Gassman,
V. A. Husak, and H. P. Greisler, "The temporal and spatial dynamics
of microscale collagen scaffold remodeling by smooth muscle cells,"
Biomaterials, vol. 30, no. 11, pp. 2023-2031, Apr. 2009.
[5] H. S. Kim, J. P. Schulze, A. C. Cone, G. E. Sosinsky, and M. E. Martone,
"Multi-channel transfer function with dimensionality reduction," in Conference
on Visualization and Data Analysis, IS&T/SPIEŠs International
Symposium on Electronic Imaging, 2010, pp. 1-12.
[6] J. Y. Bouguet, "Pyramidal implementation of the Lukas Kanade feature
tracker: Description of the algorithm," In Intel Research Laboratory,
Technical Report, pp. 1-9, 1999.
[7] J. Shi and C. Tomasi, "Good features to track," in Proceedings of
Computer Vision and Pattern Recognition, 1994, pp. 593-600.
[8] H. Foroosh, J. B. Zerubia, and M. Berthod, "Extension of phase correlation
to subpixel registration," IEEE Transactions on Image Processing,
vol. 14, no. 1, pp. 12-22, 2002.
[9] M. A. Fischler and R. C. Bolles, "Random sample and consensus:
A paradigm for model fitting with application to image analysis and
automated cartography," Comm. of the ACM, vol. 24, no. 6, pp. 381-
395, 1981.
[10] A. Bevilacqua, A. Gherardi, L. Carozza, and F. Piccinini, "Semiautomatic
background detection in microscopic images," in International
Conference on Biological Science and Engineering (ICBSE), Venice,
Italy, November 24-26, 2010.
[11] P. Azzari and A. Bevilacqua, "Joint spatial and tonal mosaic alignment
for motion detection with ptz camera," Lecture Notes in Computer
Science, vol. 4142, pp. 764-775, 2006.
[12] E. Dougherty, An Introduction to Morphological Image Processing.
SPIE-International Society for Optical Engine, Feb. 1992.
[1] I. Tognarini, S. Sorace, R. Zonefrati, G. Galli, A. Gozzini, S. C. Sala,
G. Thyrion, A. Carossino, A. Tanini, C. Mavilia, C. Azzari, F. Sbaiz,
A. Facchini, R. Capanna, and M. Brandi, "In vitro differentiation of
human mesenchymal stem cells on ti6al4v surfaces," Biomaterials,
vol. 29, pp. 809-824, 2008.
[2] K. S. Fubito Toyama and J. Miyamichi, "Image mosaicing from a set of
images without configuration information," in Proceedings of the 17th
International Conference on Pattern Recognition (ICPRŠ04), Cambridge
UK, August 23, vol. 2, 2004, pp. 899-902.
[3] K. Loewke, D. Camarillo, W. Piyawattanametha, D. Breeden, and
K. Salisbury, "Real-time image mosaicing with a hand-held dual-axes
confocal microscope," in Proceedings of the SPIE Conf. on Endoscopic
Microscopy III, San Jose, CA, USA, January 20, vol. 6851, 2008, pp.
1-9.
[4] Y. Pang, A. A. Ucuzian, A. Matsumura, E. M. Brey, A. A. Gassman,
V. A. Husak, and H. P. Greisler, "The temporal and spatial dynamics
of microscale collagen scaffold remodeling by smooth muscle cells,"
Biomaterials, vol. 30, no. 11, pp. 2023-2031, Apr. 2009.
[5] H. S. Kim, J. P. Schulze, A. C. Cone, G. E. Sosinsky, and M. E. Martone,
"Multi-channel transfer function with dimensionality reduction," in Conference
on Visualization and Data Analysis, IS&T/SPIEŠs International
Symposium on Electronic Imaging, 2010, pp. 1-12.
[6] J. Y. Bouguet, "Pyramidal implementation of the Lukas Kanade feature
tracker: Description of the algorithm," In Intel Research Laboratory,
Technical Report, pp. 1-9, 1999.
[7] J. Shi and C. Tomasi, "Good features to track," in Proceedings of
Computer Vision and Pattern Recognition, 1994, pp. 593-600.
[8] H. Foroosh, J. B. Zerubia, and M. Berthod, "Extension of phase correlation
to subpixel registration," IEEE Transactions on Image Processing,
vol. 14, no. 1, pp. 12-22, 2002.
[9] M. A. Fischler and R. C. Bolles, "Random sample and consensus:
A paradigm for model fitting with application to image analysis and
automated cartography," Comm. of the ACM, vol. 24, no. 6, pp. 381-
395, 1981.
[10] A. Bevilacqua, A. Gherardi, L. Carozza, and F. Piccinini, "Semiautomatic
background detection in microscopic images," in International
Conference on Biological Science and Engineering (ICBSE), Venice,
Italy, November 24-26, 2010.
[11] P. Azzari and A. Bevilacqua, "Joint spatial and tonal mosaic alignment
for motion detection with ptz camera," Lecture Notes in Computer
Science, vol. 4142, pp. 764-775, 2006.
[12] E. Dougherty, An Introduction to Morphological Image Processing.
SPIE-International Society for Optical Engine, Feb. 1992.
@article{"International Journal of Electrical, Electronic and Communication Sciences:59720", author = "Alessandro Bevilacqua and Alessandro Gherardi and Filippo Piccinini", title = "Multichannel Image Mosaicing of Stem Cells", abstract = "Image mosaicing techniques are usually employed to offer researchers a wider field of view of microscopic image of biological samples. a mosaic is commonly achieved using automated microscopes and often with one “color" channel, whether it refers to natural or fluorescent analysis. In this work we present a method to achieve three subsequent mosaics of the same part of a stem cell culture analyzed in phase contrast and in fluorescence, with a common non-automated inverted microscope. The mosaics obtained are then merged together to mark, in the original contrast phase images, nuclei and cytoplasm of the cells referring to a mosaic of the culture, rather than to single images. The experiments carried out prove the effectiveness of our approach with cultures of cells stained with calcein (green/cytoplasm and nuclei) and hoechst (blue/nuclei) probes.
", keywords = "Microscopy, image mosaicing, fluorescence, stem cells.", volume = "4", number = "11", pages = "1654-4", }
