In vivo Iron Availability and Profile Lipid Composition in Anemic Rats Fed on Diets with Black Rice Bran Extract

Iron is an essential nutrient with limited
bioavailability. Nutritional anemia caused mainly by iron deficiency
is the most recognized nutritional problem in both countries as well
as affluent societies. Rice (Oryza sativa L.) has become the most
important cereal crop for the improvement of human health due to the
starch, protein, oil, and the majority of micronutrients, particularly in
Asian countries. In this study, the iron availability and profile lipid
were evaluated for the extracts from Cibeusi varieties (black rices) of
ancient rice brans.
Results: The quality of K, B, R, E diets groups shows the same
effect on the growth of rats. Hematocrit and MCHC levels of rats fed
K, B, R and E diets were not significantly (P<0.05). MCV and MCH
levels of rats K, B, R were significantly (P<0.05) with E groups but
rats K, B, R were not significantly (P<0.05). The iron content in the
serum of rats fed with K, B, R and E diets were not significantly
(P<0.05). The highest level of iron in the serum was founded in the B
group. The iron content in the liver of rats fed with K, B, R and E
diets were not significantly (P<0.05). The highest level of iron in the
liver was founded in the R group. HDL cholesterol levels were
significantly (P<0.05) between rats of fed B, E with K, R, but K and
R were not significantly (P<0.05). LDL cholesterol levels of rats fed
K and E significantly (P<0.05) with B and R.
Conclusions: the bran of pigmented rice varieties has, with some
exceptions, greater antioxidant and free-radical scavenging activities.
The results also show that pigmented rice extracts acted as prooxidants
in the lipid peroxidation assay, possibly by mechanisms
described for the pro-oxidant activities of tocopherol and ascorbic.
Pigmented rice bran extracts more effectively increases iron stores
and reduces the prevalence of iron deficiency.

• E. P. Nurlaili
• M. Astuti
• Y. Marsono
• S. Naruki

• Anemia
• black rice bran extract
• iron
• profile lipid.

[1] Anonym, (2002). Black Rice Concentrate, A Natural Source of
Bioavailable Iron and Antioxidants. [email protected].;
www.dracoherbs.com.
[2] Liu, R. H. (2007). Whole grain phytochemicals and health. Journal of
Cereal Science, 46, 207–219.
[3] Butsat, S., and Siriamornpun, S. (2010). Antioxidant capacities and
phenolic compounds of the husk, bran and endosperm of Thai rice. Food
Chemistry, 119, 606–613.
[4] Aguilar-Garcia, C., Gavino, G., Baragano-Mosqueda, M., Hevia, P., and
Gavino, V. (2007). Correlation of tocopherol, tocotrienol, c-oryzanol
and total polyphenol content in rice bran with different antioxidant
capacity assays. Food Chemistry, 102, 1228–1232.
[5] Adom, K. K., and Liu, R. H. (2002). Antioxidant activity of grains.
Journal of Agricultural and Food Chemistry, 50, 6182–6187.
[6] Yawadio, R., Tanimori, S., and Morita, N. (2007). Identification of
phenolic compounds isolated from pigmented rices and their aldose
reductase inhibitory activities. Food Chemistry, 101, 1616–1625
[7] Miller, A., and Engel, K.H. (2006). Content of c-oryzanol and
composition of sterylferulates in brown rice (Oryza sativa L.) of
European origin. Journal of Agricultural and Food Chemistry, 54, 8127–
8133.
[8] Nam, S. H., Choi, S. P., Kang, M. Y., Kozukue, N., & Friedman, M.
(2005). Antioxidative, antimutagenic, and anticarcinogenic activities of
rice bran extracts in chemical and cell assays. Journal of Agricultural
and Food Chemistry, 53, 816–822
[9] Ling WH, Wang L.L, Ma J. (2002). Supplementation of the black rice
outer layer fraction to rabbits decreases atherosclerotic plaque formation
and increases antioxidant status. J Nutr 132: 20–26.
[10] Xia M., Ling WH., Ma J, Kitts D.D., Zawistowski J. (2003).
Supplementation of diets with the black rice pigment fraction attenuates
atherosclerotic plaque formation in apolipoprotein E deficient mice. J
Nutr 133: 744–751
[11] Stohs SJ, Bagghi D. (1995). Oxidative mechanisms in the toxicity of
metal ions. Free Rad BiolMed.; 18:321-36.
[12] Agil A, Fuller CJ, Jialal I. (1995). Susceptibility of plasma to ferrous
iron/hydrogen peroxide- mediated oxidation: demonstration of a possible
Fenton reaction. Clin. Chem; 41:220-5.
[13] Balla G, Jacob HS, Eaton JW, Belcher JD, Vercellotti GM. (1991).
Haemin: a possible physiological mediator of low density lipoprotein
oxidation and endothelial injury. Arterioscler Thromb; 11:1700-11.
[14] Herhsko C. (1989). Mechanism of iron toxicity and its possible role in
red cell membrane damage. SeminHematol; 26:277-85.
[15] Jacobs P, Wormald L. (1979). The bioavailability of an iron polymaltose
complex for treatment of iron deficiency. Journal of Medicine;
10(4):279-285.
[16] Buettner, G. R. (1986). Ascorbate autoxidation in the presence of iron ad
cnopper chelates. Free Radical Research Communications, 1(6), 349–
353.
[17] Yamauchi, R., Miyake, N., Kato, K., & Ueno, Y. (1993). Reaction of atocopherol
with alkyl and alkylperoxyl radicals of methyl inoleate.
Lipids, 28(3), 201–206.