In vitro Environmental Factors Controlling Root Morphological Traits of Pineapple (Ananas comosus L. Merr)
Developing our knowledge of when pineapple roots
grow can lead to improved water, fertilizer applications, and more
precise culture management. This paper presents current
understanding of morphological traits in pineapple roots, highlighting
studies using incubation periods and various solid MS media treated
with different sucrose concentrations and pH, which directly assess in
vitro environmental factors. Rooting parameters had different optimal
sucrose concentrations and incubation periods. All shoots failed to
root in medium supplemented with sucrose at 5 g/L and no roots
formed within the first 45 days in medium enriched with sucrose at
10 g/L. After 75 days, all shoots rooted in medium enriched with 10
and 20 g/L sucrose. Moreover, MS medium supplied with 20 g/L
sucrose resulted in the longest and the highest number of roots with
27.3 mm and 4.7, respectively. Root function, such as capacity for P
and N uptake, declined rapidly with root length. As a result, the
longer the incubation period, the better the rooting responses would
be.
[1] Hamad, A. H. A., Taha, R. M., Mohajer, S. 2013. In vitro Induction and
Proliferation of Adventitious Roots in Pineapple (Ananas comosus L.)
Cultivars of Smooth Cayenne and Morris. Australian Journal of Crop
Science, 7(7): 1038-1045.
[2] Xuan, W., Zhu, F. Y., Xu, S. H., Huang, B. K., Ling, T. L., Qi, J. Y.
(2008). The HemeOxygenase/carbon monoxide system is involved in the
auxin-induced cucumber adventitious rooting process. Plant Physiol,
148:881-893.
[3] Soneji, J. R., Rao, P. S., Mhatre, M. 2002. Somaclonal variation in
micropropagated dormant axillary buds of pineapple (Ananas-Comosus
L., Merr.). J. hort. sci. biotech. 77 (1): 28-32.
[4] Ko, H. L., Campbell, P. R., Jobin-Décor, M. P., Eccleston, K. L.,
Graham, M. W., Smith, M. K., 2006. The introduction of transgenes to
control blackheart in pineapple (Ananas comosus L.) cv. Smooth
cayenne by microprojectile bombardment. Euphytica, 150: 387- 395.
[5] Bhatia, P., Ashwath, N. 2002. Development of rapid method for
micropropagation of a new pineapple (Ananas comosus (L) Merr. Clone
Yeppoon gold. Acta Hort. 575: 125- 131.
[6] Almeida, W. A., DeSantana, G. S., Rodriguez, A. P. M., Costa, M. A. P.
2002. Optimization of a protocol for the micropropagation of pineapple.
Rev. Brasil. Fruticult. 24 (2), 296- 300.
[7] Khan, S., Nasib, A., Saeed, B. A. 2004. Employment of in vitro
technology for large scale multiplication of pineapples (Ananas
comosus). Pak.J. Bot. 36 (3): 611-615.
[8] Gangopadhyay, G., Bandyopadhyay, T., Poddar, R., Gandopadhyay, S.
B., Mukherjee, K. K. 2005. Encapsulation of pineapple micro shoots in
alginate beads for temporary storage. Curr. Sci. 88 (6): 972- 977.
[9] Hamad, A. M., Taha, R. M. 2008. The effect of different hormone and
incubation periods on in vitro proliferation of pineapple (Ananas
comusus L.Merr) cv. Smooth cayenne shoot tip culture. Pak. J. Biol. Sci.
11 (3): 386- 391.
[10] Kofi, O. F., Adachi, T. 1993. Effect of cytokinin on the proliferation of
multiple shoots of pineapple in vitro. SABRAO Journal. 25(1), 59- 69.
[11] Almeida, W. A., Matos, A. P., Souza, A. S. 1997. Effect of
benzylaminopurine (BAP) on in vitro proliferation of pineapple (Ananas
comosus (L) Merr). Acta Hort. 425, 235-242. [12] Kanso, K. E., Ayeh, K. O., Oduro, V., Amiteye, S., Amoatey, H. M.
2008. Effect of 6-benzylaminopurine and naphthalene acetic acid on in
vitro production of MD2 pineapple planting materials. World Appl. Sci.
J. 3(4): 614- 619.
[13] Escalona, M., Lorenzo, J. C., Gonzalez, B., Daquinta, M., Gonzalez, J.
L., Desjardins Y., Borroto, C. G. 1999. Pineapple (Ananas comosus L.
Merr) micropropagation in temporary immersion systems. Plant Cell
Report 18(9):743-748.
[14] Be, L. V., Debergh, P. C. 2006. Potential low cost micropropagation of
pineapple (Ananas comosus). S. Afr. J. Bot. 72: 191- 194.
[15] Kodym, A., Hollenthoner, S., Zapata-Arias, F. J. 2001. Cost reduction in
the micropropagation of banana by using tubular skylights as source for
natural lighting. In Vitro Cell. Devel. Biol. Plant, 37 (2): 237-242.
[16] Yadav, S., Saini, N., Jain, R. K. 2004. Low-cost multiplication and
RAPD analysis of micropropagated plants in sugarcane. Physiology and
Molecular Biology Plants, 10 (2), 269-276.
[17] Belarmino, M. M., Gabon, C. F. 1999. Low-cost micropropagation of
Chrysanthemum (Chrysanthemum morifolium L.) through tissue culture.
Philip. J. Sci., 128(2), 125-143.
[18] Mathews, V. H., Rangan, T. S. 1979. Multiple plantlets in lateral bud
and leaf explant in vitro cultures of pineapple. Sci. Hort. 11(4), 319-
328.
[19] Bergmann, B. A., Whetten, R. 1998. In vitro rooting and early
greenhouse growth of micropropagated Paulownia elongate shoots. New
Forests. 15: 127- 138.
[20] Konan, E. k., Kouadio, J. Y., Flori, A. 2007. Evidence for an interaction
effect during in vitro rooting of oil palm (Elaeis guineensis Jacq.)
somatic embryo-derived plantlets. In Vitro Cell. Dev. Biol. Plant. 43;
456- 466.
[1] Hamad, A. H. A., Taha, R. M., Mohajer, S. 2013. In vitro Induction and
Proliferation of Adventitious Roots in Pineapple (Ananas comosus L.)
Cultivars of Smooth Cayenne and Morris. Australian Journal of Crop
Science, 7(7): 1038-1045.
[2] Xuan, W., Zhu, F. Y., Xu, S. H., Huang, B. K., Ling, T. L., Qi, J. Y.
(2008). The HemeOxygenase/carbon monoxide system is involved in the
auxin-induced cucumber adventitious rooting process. Plant Physiol,
148:881-893.
[3] Soneji, J. R., Rao, P. S., Mhatre, M. 2002. Somaclonal variation in
micropropagated dormant axillary buds of pineapple (Ananas-Comosus
L., Merr.). J. hort. sci. biotech. 77 (1): 28-32.
[4] Ko, H. L., Campbell, P. R., Jobin-Décor, M. P., Eccleston, K. L.,
Graham, M. W., Smith, M. K., 2006. The introduction of transgenes to
control blackheart in pineapple (Ananas comosus L.) cv. Smooth
cayenne by microprojectile bombardment. Euphytica, 150: 387- 395.
[5] Bhatia, P., Ashwath, N. 2002. Development of rapid method for
micropropagation of a new pineapple (Ananas comosus (L) Merr. Clone
Yeppoon gold. Acta Hort. 575: 125- 131.
[6] Almeida, W. A., DeSantana, G. S., Rodriguez, A. P. M., Costa, M. A. P.
2002. Optimization of a protocol for the micropropagation of pineapple.
Rev. Brasil. Fruticult. 24 (2), 296- 300.
[7] Khan, S., Nasib, A., Saeed, B. A. 2004. Employment of in vitro
technology for large scale multiplication of pineapples (Ananas
comosus). Pak.J. Bot. 36 (3): 611-615.
[8] Gangopadhyay, G., Bandyopadhyay, T., Poddar, R., Gandopadhyay, S.
B., Mukherjee, K. K. 2005. Encapsulation of pineapple micro shoots in
alginate beads for temporary storage. Curr. Sci. 88 (6): 972- 977.
[9] Hamad, A. M., Taha, R. M. 2008. The effect of different hormone and
incubation periods on in vitro proliferation of pineapple (Ananas
comusus L.Merr) cv. Smooth cayenne shoot tip culture. Pak. J. Biol. Sci.
11 (3): 386- 391.
[10] Kofi, O. F., Adachi, T. 1993. Effect of cytokinin on the proliferation of
multiple shoots of pineapple in vitro. SABRAO Journal. 25(1), 59- 69.
[11] Almeida, W. A., Matos, A. P., Souza, A. S. 1997. Effect of
benzylaminopurine (BAP) on in vitro proliferation of pineapple (Ananas
comosus (L) Merr). Acta Hort. 425, 235-242. [12] Kanso, K. E., Ayeh, K. O., Oduro, V., Amiteye, S., Amoatey, H. M.
2008. Effect of 6-benzylaminopurine and naphthalene acetic acid on in
vitro production of MD2 pineapple planting materials. World Appl. Sci.
J. 3(4): 614- 619.
[13] Escalona, M., Lorenzo, J. C., Gonzalez, B., Daquinta, M., Gonzalez, J.
L., Desjardins Y., Borroto, C. G. 1999. Pineapple (Ananas comosus L.
Merr) micropropagation in temporary immersion systems. Plant Cell
Report 18(9):743-748.
[14] Be, L. V., Debergh, P. C. 2006. Potential low cost micropropagation of
pineapple (Ananas comosus). S. Afr. J. Bot. 72: 191- 194.
[15] Kodym, A., Hollenthoner, S., Zapata-Arias, F. J. 2001. Cost reduction in
the micropropagation of banana by using tubular skylights as source for
natural lighting. In Vitro Cell. Devel. Biol. Plant, 37 (2): 237-242.
[16] Yadav, S., Saini, N., Jain, R. K. 2004. Low-cost multiplication and
RAPD analysis of micropropagated plants in sugarcane. Physiology and
Molecular Biology Plants, 10 (2), 269-276.
[17] Belarmino, M. M., Gabon, C. F. 1999. Low-cost micropropagation of
Chrysanthemum (Chrysanthemum morifolium L.) through tissue culture.
Philip. J. Sci., 128(2), 125-143.
[18] Mathews, V. H., Rangan, T. S. 1979. Multiple plantlets in lateral bud
and leaf explant in vitro cultures of pineapple. Sci. Hort. 11(4), 319-
328.
[19] Bergmann, B. A., Whetten, R. 1998. In vitro rooting and early
greenhouse growth of micropropagated Paulownia elongate shoots. New
Forests. 15: 127- 138.
[20] Konan, E. k., Kouadio, J. Y., Flori, A. 2007. Evidence for an interaction
effect during in vitro rooting of oil palm (Elaeis guineensis Jacq.)
somatic embryo-derived plantlets. In Vitro Cell. Dev. Biol. Plant. 43;
456- 466.
@article{"International Journal of Biological, Life and Agricultural Sciences:71146", author = "S. Mohajer and R. M. Taha and M. Adel", title = "In vitro Environmental Factors Controlling Root Morphological Traits of Pineapple (Ananas comosus L. Merr)", abstract = "Developing our knowledge of when pineapple roots
grow can lead to improved water, fertilizer applications, and more
precise culture management. This paper presents current
understanding of morphological traits in pineapple roots, highlighting
studies using incubation periods and various solid MS media treated
with different sucrose concentrations and pH, which directly assess in
vitro environmental factors. Rooting parameters had different optimal
sucrose concentrations and incubation periods. All shoots failed to
root in medium supplemented with sucrose at 5 g/L and no roots
formed within the first 45 days in medium enriched with sucrose at
10 g/L. After 75 days, all shoots rooted in medium enriched with 10
and 20 g/L sucrose. Moreover, MS medium supplied with 20 g/L
sucrose resulted in the longest and the highest number of roots with
27.3 mm and 4.7, respectively. Root function, such as capacity for P
and N uptake, declined rapidly with root length. As a result, the
longer the incubation period, the better the rooting responses would
be.", keywords = "Environmental factors, in vitro rooting, pineapple,
tissue culture.", volume = "9", number = "11", pages = "1155-4", }