Impact of Altered Behavioral Condition on Markers of Oxidative Stress and Different Biochemical Parameters
Aim- Study was undertaken to investigate the effect of altered behavioral condition like depression on various oxidative stress markers and biochemical parameters in rats. Methods- Rats were subjected for short (21 days) and long term (84 days) social isolation; the rats displayed an increase in depression on force swim test relative to control. Various markers of oxidative stress like lipid per oxidation (LPO), reduced glutathione (GSH), Supers oxide dismutase (SOD), catalase (CAT) and biochemical parameters like SGOT, SGPT, and blood glucose were determined. Results- There was significant increase in the level of LPO and decrease in the levels of GSH, SOD and CAT after long term isolation. Biochemical parameters were significantly altered after social isolation. Conclusion- Increased oxidative stress in depression which may leads to alteration of biochemical parameters.
[1] Antigoni Fountouki, Depression in the Elderly: Limits and Challenges - a Nursing Perspective,International Journal of Caring Sciences , 2009; Vol 2, Issue 3, 108-117.
[2] J. T. Coyle and P. Puttfarcken, "Oxidative stress, glutamate, and neurodegenerative disorders,” Science, 1993; vol. 262, no. 5134, pp. 689–695.
[3] A. Gardner and R. G. Boles, "Beyond the serotonin hypothesis: mitochondria, inflammation and neurodegeneration in major depression and affective spectrum disorders,” Progress in Neuro-Psychopharmacology and Biological Psychiatry, 2011; vol. 35,no. 3, pp. 730–743,.
[4] K. Chopra, B. Kumar, and A. Kuhad, "Pathobiological targets of depression,” Expert Opinion on Therapeutic Targets, 2011, vol. 15, no. 4, pp. 379–400, 2011.
[5] Celeste Bolin and Fernando Cardozo-Pelaez. Assessing Biomarkers of Oxidative Stress: Analysis of Guanosine and Oxidized Guanosine Nucleotide Triphosphates by High Performance Liquid Chromatography with Electrochemical Detection.NIH Public Acess 2007. Page No 121-130
[6] Sycanlarda Sosyal, Yzolasyonun Oksidan, Stres ve, Eritrosit Deformabilitesi, Uzerine Etkileri, Consequences of Social Isolation in Rats on Their Antioxidant Defense System and Erythrocyte Deformability, Erciyes Typ Dergisi (Erciyes Medical Journal) 2009; 31(1):009-014
[7] L. Gavrilovic, N. Spasojevic, M. Zivkovic and S. Dronjak, Effect of immobilization stress on gene expression of catecholamine biosynthetic enzymes in heart auricles of socially isolated rats, Braz J Med Biol Res 2009; 42(12): 1185-1190
[8] S. Bajaj, and S. B. Vohora, Anti-Cataleptic, Anti-Anxiety And Anti-Depressant Activity Of Gold Preparations Used In Indian Systems Of Medicine Indian J. Pharmacology, 2000, 32(3), 339-346)
[9] Fedra R. Hinojosa, Luiz Spricigo Jr., Geison S. Izidio, Gustavo R. Bruske, Douglas M. Lopes, Andre Ramos, Evaluation of two genetic animal models in behavioral tests of anxiety and depression, Behavioural Brain Research 2006, 168, 127–136
[10] J Stocks and TL. Dormandy The autoxidation of human red cell lipids induced by hydrogen peroxide. Br. J. Hematol., 1971; 20: 95.
[11] H Aebi . Catalase in vitro. Methods Enzymol. 1984; 105:121.
[12] J McCord and I. Fridovich Superoxide dismutase. An enzymic function for erythrocuprein (hemocuprein). J. Biol. Chem., 1969, 244(5): 6049-605.
[13] G. L Ellman., Tissue sulfhydryl groups. Arch. Biochem. Biphys. 1959, 82: 70.
[14] M S Osama, E Rawhia, G E Harisa, Experimental Diabetic Nephropathy Can Be Prevented By Propolis: Effect on Metabolic Disturbances And Renal Oxidative Parameters Pak. J. Pharm. Sci., 2009; 22( 2) : 205-210.
[15] SF Ambali, K Shuaib R, Edeh, BC Orieji, M Shittu, M Akande Hyperglycemia induced by subchronic co-administration of chlorpyrifos and lead in Wistar rats: Role of pancreatic lipoperoxidation and alleviating effect of vitamin C, Biology and Medicine, 2011; 3 (1): 6-14.
[16] S. Reitman, S. Frankel, A colorimetric method for the determination of serum glutamic oxaloacetic acid and glutamic pyruvic transaminases. American Journal of Clinical Pathology 1957; 28: 56–63.
[17] L. Macho, D. J. Ezova, S. Zorad, M. Fickova, "Postnatal Monosodium Glutamate treatment results in attenuation of corticosterone metabolic rate in adult rats” Endocrine regulations, 1999;33:61-67.
[1] Antigoni Fountouki, Depression in the Elderly: Limits and Challenges - a Nursing Perspective,International Journal of Caring Sciences , 2009; Vol 2, Issue 3, 108-117.
[2] J. T. Coyle and P. Puttfarcken, "Oxidative stress, glutamate, and neurodegenerative disorders,” Science, 1993; vol. 262, no. 5134, pp. 689–695.
[3] A. Gardner and R. G. Boles, "Beyond the serotonin hypothesis: mitochondria, inflammation and neurodegeneration in major depression and affective spectrum disorders,” Progress in Neuro-Psychopharmacology and Biological Psychiatry, 2011; vol. 35,no. 3, pp. 730–743,.
[4] K. Chopra, B. Kumar, and A. Kuhad, "Pathobiological targets of depression,” Expert Opinion on Therapeutic Targets, 2011, vol. 15, no. 4, pp. 379–400, 2011.
[5] Celeste Bolin and Fernando Cardozo-Pelaez. Assessing Biomarkers of Oxidative Stress: Analysis of Guanosine and Oxidized Guanosine Nucleotide Triphosphates by High Performance Liquid Chromatography with Electrochemical Detection.NIH Public Acess 2007. Page No 121-130
[6] Sycanlarda Sosyal, Yzolasyonun Oksidan, Stres ve, Eritrosit Deformabilitesi, Uzerine Etkileri, Consequences of Social Isolation in Rats on Their Antioxidant Defense System and Erythrocyte Deformability, Erciyes Typ Dergisi (Erciyes Medical Journal) 2009; 31(1):009-014
[7] L. Gavrilovic, N. Spasojevic, M. Zivkovic and S. Dronjak, Effect of immobilization stress on gene expression of catecholamine biosynthetic enzymes in heart auricles of socially isolated rats, Braz J Med Biol Res 2009; 42(12): 1185-1190
[8] S. Bajaj, and S. B. Vohora, Anti-Cataleptic, Anti-Anxiety And Anti-Depressant Activity Of Gold Preparations Used In Indian Systems Of Medicine Indian J. Pharmacology, 2000, 32(3), 339-346)
[9] Fedra R. Hinojosa, Luiz Spricigo Jr., Geison S. Izidio, Gustavo R. Bruske, Douglas M. Lopes, Andre Ramos, Evaluation of two genetic animal models in behavioral tests of anxiety and depression, Behavioural Brain Research 2006, 168, 127–136
[10] J Stocks and TL. Dormandy The autoxidation of human red cell lipids induced by hydrogen peroxide. Br. J. Hematol., 1971; 20: 95.
[11] H Aebi . Catalase in vitro. Methods Enzymol. 1984; 105:121.
[12] J McCord and I. Fridovich Superoxide dismutase. An enzymic function for erythrocuprein (hemocuprein). J. Biol. Chem., 1969, 244(5): 6049-605.
[13] G. L Ellman., Tissue sulfhydryl groups. Arch. Biochem. Biphys. 1959, 82: 70.
[14] M S Osama, E Rawhia, G E Harisa, Experimental Diabetic Nephropathy Can Be Prevented By Propolis: Effect on Metabolic Disturbances And Renal Oxidative Parameters Pak. J. Pharm. Sci., 2009; 22( 2) : 205-210.
[15] SF Ambali, K Shuaib R, Edeh, BC Orieji, M Shittu, M Akande Hyperglycemia induced by subchronic co-administration of chlorpyrifos and lead in Wistar rats: Role of pancreatic lipoperoxidation and alleviating effect of vitamin C, Biology and Medicine, 2011; 3 (1): 6-14.
[16] S. Reitman, S. Frankel, A colorimetric method for the determination of serum glutamic oxaloacetic acid and glutamic pyruvic transaminases. American Journal of Clinical Pathology 1957; 28: 56–63.
[17] L. Macho, D. J. Ezova, S. Zorad, M. Fickova, "Postnatal Monosodium Glutamate treatment results in attenuation of corticosterone metabolic rate in adult rats” Endocrine regulations, 1999;33:61-67.
@article{"International Journal of Medical, Medicine and Health Sciences:66308", author = "D. S. Mohale and A. V. Chandewar", title = "Impact of Altered Behavioral Condition on Markers of Oxidative Stress and Different Biochemical Parameters", abstract = "Aim- Study was undertaken to investigate the effect of altered behavioral condition like depression on various oxidative stress markers and biochemical parameters in rats. Methods- Rats were subjected for short (21 days) and long term (84 days) social isolation; the rats displayed an increase in depression on force swim test relative to control. Various markers of oxidative stress like lipid per oxidation (LPO), reduced glutathione (GSH), Supers oxide dismutase (SOD), catalase (CAT) and biochemical parameters like SGOT, SGPT, and blood glucose were determined. Results- There was significant increase in the level of LPO and decrease in the levels of GSH, SOD and CAT after long term isolation. Biochemical parameters were significantly altered after social isolation. Conclusion- Increased oxidative stress in depression which may leads to alteration of biochemical parameters.
", keywords = "Depression, Glucose, LPO, Oxidative stress.", volume = "7", number = "12", pages = "992-5", }
