Field Investigation on Modification of Japanese Cedar Pollen Allergen in Urban Air-Polluted Area

Cry j 1 is a causative substance of Japanese cedar pollinosis, and it may deteriorate by Cry j 1 invasion to a lower respiratory tract. We observed airborne particles containing Cry j 1 by an immunofluorescence technique using a fluorescence microscope, and we clarified that Cry j 1 exist as aggregates of airborne fine particles (< 1.1 μm) in the urban atmosphere. Airborne Cry j 1 may react with air pollutants and be denature to a substance deteriorated Japanese cedar pollinosis. Therefore, we applied a sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) to evaluate a Cry j 1 reacted with various air pollutants by liquid phase reaction, and calculated kinetics constants of Cry j 1 extracted from pollens collected in various sites and airborne fine particles containing Cry j 1 by using a surface plasmon resonance (SPR) method. As a result, it is suggested that Cry j 1 may be denatured by air pollutants during the transportation to the urban atmosphere.




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Samples
(sites)
Ka (1/Ms) Kd (1/s) KD (M)
Cry j 1concentrations
(╬╝g/mL)
Pollen
(Saitama) 4.76×105 8.51×10-4 1.79×10-9 6.29 ┬▒ 0.231
Pollen
(Ibaraki) 2.44×106 3.21×10-3 1.32×10-9 6.23 ┬▒ 0.256
Pollen
(Tama) 1.76×105 5.92×10-4 3.37×10-9 6.58 ┬▒ 0.581
PM1.1
(Saitama) 2.22×108 3.90×10-6 1.76×10-14 ´╝ì
114.0
84.7
47.3
31.3
25.7
1 2 3
Molecular
Weight
(kDa)
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