Effect of Various Concentrations of Humic Acid on Growth and Development of Eggplant Seedlings in Tissue Cultures at Low Nutrient Level
Humic acids (HAs) have been shown to activate some
ion uptakes along with stimulating the lateral roots at effective
concentration of micronutrients. However, the effects of HA on ion
adsorption by plant roots are not easily explainable due to the
varieties of HAs that differ from origins. Therefore, this study was
aimed to investigate the effect of various concentrations of HA
obtained from the compost derived from mix manures and some
agricultural wastes on the growth of eggplant seedlings (Solanum
melongena L. cv. Chao Praya) in tissue cultures at low nutrient level.
Egg plant seeds were surfaced sterilized and germinated in ½
Murashige and Skoog medium (MS) without HA added or in ¼ MS
supplemented with 0, 25, 50, 75 and 100 ppm of HAs. Then, they
were cultured for 4 weeks under the controlled environment. The
results showed that seedlings grown on ¼MS supplemented with
HAs at the concentration of 25 and 50 ppm had the average plant
heights (2.49 and 2.28 cm, respectively) higher than the other
treatments. Both treatments also significantly showed the maximum
average fresh and dry weights (p<0.05). Also the later yielded the
highest average number of leaves and the longest average root length
(p<0.05). However, there was no statistically different in the number
of roots among treatments (p>0.05). This suggested that HAs at the
concentration of 25 and 50 ppm could improve the growth of egg
plant seedlings in tissue cultures at low nutrient level (¼ MS).
[1] S. Nardi, D. Pizzeghello, A. Muscolo, and A. Vianello, "Physiological
effects of humic substances on higher plants," Soil Biol. Biocchem.,
vol. 34, pp. 1527- 1536, 2002.
[2] B. Eyheraguibel, J. Silvestre, and P, Morard, "Effects of humic
substances derived from organic waste enhancement on the growth and
mineral nutrition of maize," Bioresource Techno.l, vol. 99, pp. 4206-
4212, 2008.
[3] M.D. Lulakis, and S.I. Petsas, "Effect of humic substances from vinecanes
mature compost on tomato seedling growth," Bioresource
Technol., vol. 54, pp. 179-182,1995.
[4] Y.S. Lee, and R.J. Bartlett, "Stimulation of plant growth by humic
substances," Soil Sci. Soc. Am. J., vol. 40, pp. 876-879, 1976.
[5] J.R. Brownell, G. Nordstrom, J. Marihart, and G. Jorgrnsen, "Crop
responses from two new leonardite Extracts," Sci.Total Environ., vol.
62, pp. 491-499,1987.
[6] T. Murashige and F. Skoog, "A revised medium for rapid growth and
bioassays with tobacco tissue cultures," Physiol Plant., vol. 15 (3), pp.
473-497, 1962.
[7] International Humic Substances Society, Isolation of IHSS Samples.
Located at: http//ihss.gatech.edu/ isolation.html., 2007, Accessed 2010
Apr 8.
[8] L.P. Canellas, F.L. Olivares, A.L. Okorokova, and A.R. Facanha,
"Humic acids isolated from earthworm compost enhance root
elongation, lateral root emergence, and plasma H+-ATPase activity in
maize roots," Plant Physiol., vol. 130, pp. 1951-1957, 2000.
[9] R.M. Atiyeh, C.A. Edwards, J.D. Metzger, S. Lee, and N.Q. Arancon,
"The influence of humic acids derived from earthworm processed
organic wastes on plant growth," Bioresour. Technol., vol. 84, pp. 7-14,
2002.
[10] W.G. Hopkins and N.P.A. Huner, Introduction to plant physiology, John
Wily and Sons, Inc., New Jersey, 2004.
[1] S. Nardi, D. Pizzeghello, A. Muscolo, and A. Vianello, "Physiological
effects of humic substances on higher plants," Soil Biol. Biocchem.,
vol. 34, pp. 1527- 1536, 2002.
[2] B. Eyheraguibel, J. Silvestre, and P, Morard, "Effects of humic
substances derived from organic waste enhancement on the growth and
mineral nutrition of maize," Bioresource Techno.l, vol. 99, pp. 4206-
4212, 2008.
[3] M.D. Lulakis, and S.I. Petsas, "Effect of humic substances from vinecanes
mature compost on tomato seedling growth," Bioresource
Technol., vol. 54, pp. 179-182,1995.
[4] Y.S. Lee, and R.J. Bartlett, "Stimulation of plant growth by humic
substances," Soil Sci. Soc. Am. J., vol. 40, pp. 876-879, 1976.
[5] J.R. Brownell, G. Nordstrom, J. Marihart, and G. Jorgrnsen, "Crop
responses from two new leonardite Extracts," Sci.Total Environ., vol.
62, pp. 491-499,1987.
[6] T. Murashige and F. Skoog, "A revised medium for rapid growth and
bioassays with tobacco tissue cultures," Physiol Plant., vol. 15 (3), pp.
473-497, 1962.
[7] International Humic Substances Society, Isolation of IHSS Samples.
Located at: http//ihss.gatech.edu/ isolation.html., 2007, Accessed 2010
Apr 8.
[8] L.P. Canellas, F.L. Olivares, A.L. Okorokova, and A.R. Facanha,
"Humic acids isolated from earthworm compost enhance root
elongation, lateral root emergence, and plasma H+-ATPase activity in
maize roots," Plant Physiol., vol. 130, pp. 1951-1957, 2000.
[9] R.M. Atiyeh, C.A. Edwards, J.D. Metzger, S. Lee, and N.Q. Arancon,
"The influence of humic acids derived from earthworm processed
organic wastes on plant growth," Bioresour. Technol., vol. 84, pp. 7-14,
2002.
[10] W.G. Hopkins and N.P.A. Huner, Introduction to plant physiology, John
Wily and Sons, Inc., New Jersey, 2004.
@article{"International Journal of Biological, Life and Agricultural Sciences:57910", author = "Kullanart Obsuwan and Suluck Namchote and Natdhera Sanmanee and Kamolchanok Panishkan and Sirichai Dharmvanij", title = "Effect of Various Concentrations of Humic Acid on Growth and Development of Eggplant Seedlings in Tissue Cultures at Low Nutrient Level", abstract = "Humic acids (HAs) have been shown to activate some
ion uptakes along with stimulating the lateral roots at effective
concentration of micronutrients. However, the effects of HA on ion
adsorption by plant roots are not easily explainable due to the
varieties of HAs that differ from origins. Therefore, this study was
aimed to investigate the effect of various concentrations of HA
obtained from the compost derived from mix manures and some
agricultural wastes on the growth of eggplant seedlings (Solanum
melongena L. cv. Chao Praya) in tissue cultures at low nutrient level.
Egg plant seeds were surfaced sterilized and germinated in ½
Murashige and Skoog medium (MS) without HA added or in ¼ MS
supplemented with 0, 25, 50, 75 and 100 ppm of HAs. Then, they
were cultured for 4 weeks under the controlled environment. The
results showed that seedlings grown on ¼MS supplemented with
HAs at the concentration of 25 and 50 ppm had the average plant
heights (2.49 and 2.28 cm, respectively) higher than the other
treatments. Both treatments also significantly showed the maximum
average fresh and dry weights (p", keywords = "growth, seedling, humic acid, fresh weght, dry weight, tissue culture", volume = "5", number = "8", pages = "455-3", }