Dextran Modified Silicon Photonic Microring Resonator Sensors

We present a dextran modified silicon microring resonator sensor for high density antibody immobilization. An array of sensors consisting of three sensor rings and a reference ring was fabricated and its surface sensitivity and the limit of detection were obtained using polyelectrolyte multilayers. The mass sensitivity and the limit of detection of the fabricated sensor ring are 0.35 nm/ng mm-2 and 42.8 pg/mm2 in air, respectively. Dextran modified sensor surface was successfully prepared by covalent grafting of oxidized dextran on 3-aminopropyltriethoxysilane (APTES) modified silicon sensor surface. The antibody immobilization on hydrogel dextran matrix improves 40% compared to traditional antibody immobilization method via APTES and glutaraldehyde linkage.

Authors:

• Jessie Yiying Quah
• Vivian Netto
• Jack Sheng Kee
• Eric Mouchel La Fosse
• Mi Kyoung Park

Keywords:

• Antibody immobilization
• Dextran
• Immunosensor
• Label-free detection
• Silicon micro-ring resonator

References:

[1] A. L. Washburn, R. C. Bailey, "Photonics-on-a-chip: recent advances in
integrated waveguides as enabling detection elements for real-world,
lab-on-a-chip biosensing applications," Analyst, vol. 126, pp. 227-236,
2010.
[2] K. De Vos, I. Bartolozzi, E. Schacht, P. Bienstman, R. Baets. "Siliconon-
Insulator microring resonator for sensitive and Label-free
Biosensing, " Optical Express, v.15, pp. 7610-7615, June 2007.
[3] C. F. Carlborg, K. B. Gylfason, A. Kazmierczak, F. Dortu, M. J.
Ba~nuls Polo, A. Maquieira Catala, et al. "A packaged optical slotwaveguide
ring resonator sensor array for multiplex label-free assays in
labs-on-chips," Lap on a Chip, vol. 10, pp. 281-290, 2010.
[4] A. Densmore, M. Vachon, D.-X. Xu, S. Janz, R. Ma, Y.-H. Li, et al.
"Silicon photonic wire biosensor array for multiplexed real-time and
label-free molecular detection," Optics Letters, vol. 34, pp.3598-3600,
December 2009.
[5] S. Mandal and D. Erickson, "Nanoscale optofluidic sensor arrays," Opt.
Express, vol. 16, pp. 1623-1631, Fabruary 2010.
[6] M. Iqbal, M. A. Gleeson, B. Spaugh, F.Tybor, W. G. Gunn, M.
Hochberg, et al. "Label-Free Biosensor Arrays Based on Silicon Ring
Resonators and High-Speed Optical Scanning Instrumentation," IEEE J.
Sel. Top. Quantum Electronics, vol.16, pp. 654-661, May/June 2010.
[7] A. L. Washburn, M. S. Luchansky, A. L. Bowman, R. C. Bailey,
"Quantitative, Label-Free Detection of Five Protein Biomarkers Using
Multiplexed Arrays of Silicon Photonic Microring Resonators," Anal.
Chem., vol. 82, pp. 69-72, 2010.
[8] A. J. Qavi, J. T. Kindt, M. A. Gleeson, R. C. Bailey, "Anti-DNA:RNA
Antibodies and Silicon Photonic Microring Resonators: Increased
Sensitivity for Multiplexed microRNA Detection," Anal. Chem., vol. 83,
pp. 5949-5956, 2011.
[9] J.-O Lee, H.-M. So, E.-K. Jeon, H. Chang, K. Won, Y. H. Kim,
"Aptamers as molecular recognition elements for electrical
nanobiosensors," Anal. Bioanal. Chem. Vo. 390, pp. 1023-1032, 2008.
[10] Z. Feldötö, I. Varga, E. Blomberg, "Influence of salt and rinsing
protocol on the structure of PAH/PSS polyelectrolyte multilayers,"
Langmuir,vo.l 26, pp. 17048-17057, 2010.
[11] F. Caruso, K. Niikura, D.N. Furlong, Y. Okahata, 1. Ultrathin multilayer
polyelectrolyte films on gold: construction and thickness determination,
Langmuir, 13 (1997) 3422-3426.