Cell Growth and Metabolites Produced by Fluorescent Pseudomonad R62 in Modified Chemically Defined Medium
Chemically defined Schlegel-s medium was modified
to improve production of cell growth and other metabolites that are
produced by fluorescent pseudomonad R62 strain. The modified
medium does not require pH control as pH changes are kept within ±
0.2 units of the initial pH 7.1 during fermentation. The siderophore
production was optimized for the fluorescent pseudomonad strain in
the modified medium containing 1% glycerol as a major carbon
source supplemented with 0.05% succinic acid and 0.5% Ltryptophan.
Indole-3 acetic acid (IAA) production was higher when
L-tryptophan was used at 0.5%. The 2,4- diacetylphloroglucinol
(DAPG) was higher with amended three trace elements in medium.
The optimized medium produced 2.28 g/l of dry cell mass and 900
mg/l of siderophore at the end of 36 h cultivation, while the
production levels of IAA and DAPG were 65 mg/l and 81 mg/l
respectively at the end of 48 h cultivation.
[1] E. María and D.M.E. de Villegas, "Biotechnological production of
siderophores," in Microbial Siderophores, vol.12, A. Varma, S.
Chincholkar, Eds. Berlin, Heidelberg: Springer Verlag, 2007, pp. 219-
231.
[2] S.H. Hua T. Yoshida, Y. Meng, T. Kabashima, K. Ito, Y. Nishiya, Y.
Kawamura, and T. Yoshimoto, "Purification and characterization of
thermostable glycerol kinase from Thermus flavus," J. Ferment. Bioeng.
vol. 83, pp. 328-332, Jan.1997.
[3] D.M.E de Villegas, P. Villa, and A. Frias, "Evaluation of the
siderophores production by Pseudomonas aeruginosa PSS," Rev.
Latinoam. Microbiol, vol. 44, pp.112-117, Sep. 2002.
[4] T. Leisinger, and L.Margrafet, "Secondary metabolites of the fluorescent
pseudomonads," Microbiol Rev, vol. 43, pp. 422-442, Sep. 1979.
[5] D.J. O-Sullivan, and F. O-Gara, "Traits of fluorescent pseudomonas sp.
involved in suppression of plant root pathogens," Microbiol. Rev, vol.
56, pp 662-676, Dec.1992.
[6] R. Gaur, S. Noam, S. Kawaljeet, B.N. Johri, P. Rossi, and M. Aragno,
"Diacetylphloroglucinol producing pseudomonads do not influence AM
fungi in wheat rhizosphere," Curr. Sci, vol. 86, pp 453-457. Feb. 2004.
[7] D. Roesti, R. Gaur, B.N. Johri, G. Imfeld, S. Sharma, K. Kwaljeet, and
M. Aragno, "Plant growth stage, fertilizer management and
bioinoculation of arbuscular mycorrhizal fungi and plant growth
promoting rhizobacteria affect the rhizobacterial community structure in
rain-fed wheat fields," Soil. Biol. Biochem, vol. 38, pp 1111-1120, Jan.
2006.
[8] D. Rachid, and B. Ahmed, "Effect of iron and growth inhibitors on
siderophores production by Pseudomonas fluorescens" African J.
Biotechnol, vol. 4, pp 697-702, July 2005.
[9] M. Aragno, and H.G. Schlegel, "The mesophilic hydrogen-oxidizing
(Knallgas) bacteria," in The Prokaryotes, A. Balows, H.G. Tr├╝per, M.
Dworkin, W. Harder, and K.H. Schleifer KH, Eds. Berlin Heidelberg
New York: Springer Verlag, 1991, pp. 344-384.
[10] J.M. Meyer, and M.A. Abdallah, "The florescent pigment of
Pseudomonas fluorescens biosynthesis, purification and
physicalchemical properties," J Gen Microbiol, vol. 107, pp 319-328,
April 1978.
[11] S.A. Gordon and R.P. Weber, "Colorimetric estimation of indole-acetic
acid," Plant Physiol, vol. 26, pp. 192-195, Jan. 1951.
[12] B. Schwyn and J.B. Neilands,"Universal Chemical Assay for the
detection and determination of siderophores," Anal Biochem, vol. 140,
pp. 47-56, Jan. 1987.
[1] E. María and D.M.E. de Villegas, "Biotechnological production of
siderophores," in Microbial Siderophores, vol.12, A. Varma, S.
Chincholkar, Eds. Berlin, Heidelberg: Springer Verlag, 2007, pp. 219-
231.
[2] S.H. Hua T. Yoshida, Y. Meng, T. Kabashima, K. Ito, Y. Nishiya, Y.
Kawamura, and T. Yoshimoto, "Purification and characterization of
thermostable glycerol kinase from Thermus flavus," J. Ferment. Bioeng.
vol. 83, pp. 328-332, Jan.1997.
[3] D.M.E de Villegas, P. Villa, and A. Frias, "Evaluation of the
siderophores production by Pseudomonas aeruginosa PSS," Rev.
Latinoam. Microbiol, vol. 44, pp.112-117, Sep. 2002.
[4] T. Leisinger, and L.Margrafet, "Secondary metabolites of the fluorescent
pseudomonads," Microbiol Rev, vol. 43, pp. 422-442, Sep. 1979.
[5] D.J. O-Sullivan, and F. O-Gara, "Traits of fluorescent pseudomonas sp.
involved in suppression of plant root pathogens," Microbiol. Rev, vol.
56, pp 662-676, Dec.1992.
[6] R. Gaur, S. Noam, S. Kawaljeet, B.N. Johri, P. Rossi, and M. Aragno,
"Diacetylphloroglucinol producing pseudomonads do not influence AM
fungi in wheat rhizosphere," Curr. Sci, vol. 86, pp 453-457. Feb. 2004.
[7] D. Roesti, R. Gaur, B.N. Johri, G. Imfeld, S. Sharma, K. Kwaljeet, and
M. Aragno, "Plant growth stage, fertilizer management and
bioinoculation of arbuscular mycorrhizal fungi and plant growth
promoting rhizobacteria affect the rhizobacterial community structure in
rain-fed wheat fields," Soil. Biol. Biochem, vol. 38, pp 1111-1120, Jan.
2006.
[8] D. Rachid, and B. Ahmed, "Effect of iron and growth inhibitors on
siderophores production by Pseudomonas fluorescens" African J.
Biotechnol, vol. 4, pp 697-702, July 2005.
[9] M. Aragno, and H.G. Schlegel, "The mesophilic hydrogen-oxidizing
(Knallgas) bacteria," in The Prokaryotes, A. Balows, H.G. Tr├╝per, M.
Dworkin, W. Harder, and K.H. Schleifer KH, Eds. Berlin Heidelberg
New York: Springer Verlag, 1991, pp. 344-384.
[10] J.M. Meyer, and M.A. Abdallah, "The florescent pigment of
Pseudomonas fluorescens biosynthesis, purification and
physicalchemical properties," J Gen Microbiol, vol. 107, pp 319-328,
April 1978.
[11] S.A. Gordon and R.P. Weber, "Colorimetric estimation of indole-acetic
acid," Plant Physiol, vol. 26, pp. 192-195, Jan. 1951.
[12] B. Schwyn and J.B. Neilands,"Universal Chemical Assay for the
detection and determination of siderophores," Anal Biochem, vol. 140,
pp. 47-56, Jan. 1987.
@article{"International Journal of Biological, Life and Agricultural Sciences:49592", author = "K. Saharan and M.V. R. K. Sarma and A. S. Roesti and A. Prakash and B. N. Johri and M. Aragno and V. S. Bisaria and V. Sahai", title = "Cell Growth and Metabolites Produced by Fluorescent Pseudomonad R62 in Modified Chemically Defined Medium", abstract = "Chemically defined Schlegel-s medium was modified
to improve production of cell growth and other metabolites that are
produced by fluorescent pseudomonad R62 strain. The modified
medium does not require pH control as pH changes are kept within ±
0.2 units of the initial pH 7.1 during fermentation. The siderophore
production was optimized for the fluorescent pseudomonad strain in
the modified medium containing 1% glycerol as a major carbon
source supplemented with 0.05% succinic acid and 0.5% Ltryptophan.
Indole-3 acetic acid (IAA) production was higher when
L-tryptophan was used at 0.5%. The 2,4- diacetylphloroglucinol
(DAPG) was higher with amended three trace elements in medium.
The optimized medium produced 2.28 g/l of dry cell mass and 900
mg/l of siderophore at the end of 36 h cultivation, while the
production levels of IAA and DAPG were 65 mg/l and 81 mg/l
respectively at the end of 48 h cultivation.", keywords = "Fluorescent pseudomonad, Fermentation, Metabolites production, PGPR.", volume = "4", number = "7", pages = "442-5", }