Biosynthesis of Titanium Dioxide Nanoparticles and Their Antibacterial Property
This paper presents a low-cost, eco-friendly and reproducible microbe mediated biosynthesis of TiO2 nanoparticles. TiO2 nanoparticles synthesized using the bacterium, Bacillus subtilis, from titanium as a precursor, were confirmed by TEM analysis. The morphological characteristics state spherical shape, with the size of individual or aggregate nanoparticles, around 30-40 nm. Microbial resistance represents a challenge for the scientific community to develop new bioactive compounds. Here, the antibacterial effect of TiO2 nanoparticles on Escherichia coli was investigated, which was confirmed by CFU (Colony-forming unit). Further, growth curve study of E. coli Hb101 in the presence and absence of TiO2 nanoparticles was done. Optical density decrease was observed with the increase in the concentration of TiO2. It could be attributed to the inactivation of cellular enzymes and DNA by binding to electron-donating groups such as carboxylates, amides, indoles, hydroxyls, thiols, etc. which cause little pores in bacterial cell walls, leading to increased permeability and cell death. This justifies that TiO2 nanoparticles have efficient antibacterial effect and have potential to be used as an antibacterial agent for different purposes.
[1] G. Zhao and S. E. Stevens, “Multiple Parameters for the Comprehensive Evaluation of the Susceptibility of Escherichia coli to the Silver Ion,” BioMetals, Vol. 11, pp. 27-32, 1998.
[2] J.H. Crabtree, R.J. Burchette, R.A. Siddiqi, I.T. Huen, L.L. Hadnott, and A. Fishman, “The efficacy of silver-ion implanted catheters in reducing peritoneal dialysis-related infections,” Perit Dial Int., Vol.23, pp.368-74, 2003.
[3] A. Krolikowska, A. Kudelski, A. Michota, and J. Bukowska, “SERS Studies on the Structure of Thioglycolic Acid Monolayers on Silver and Gold,” Surf. Sci., Vol. 532, pp. 227-232, 2003.
[4] M. Catauro, M.G. Raucci, F.D. De Gaaetano, and A. Marotta, “Antibacterial and bioactive silver-containing Na2O.CaO.2SiO2 glass prepared by sol-gel method,” J. Mater. Sci. Mater. Med., Vol. 15, pp.831-837, 2004.
[5] Sun D, Meng TT, Loong H, Hwa TJ. Water Sci Technol 2004;49:103.
[6] Gelis C, Girard S, Mavon A, Delverdier M, Pailous N, Vicendo P. Photomed 2003;19: 242.
[7] Joerger R, Klaus T, Granqvist CG. Adv Mater 2000;12:407.
[8] Tolles WM, Rath BB. Curr Sci 2003;85:1746.
[9] X. Gao, Y. Cui, R.M. Levenson, L.W. Chung, and S.Nie,“In vivo cancer targeting and imaging with semiconductor quantum dots,”Nature Biotechnology, Vol. 8pp. 969-76,2004.
[10] J.M. Streit, T.R. Fritsche, H.S. Sader, and R.N. Jones, “Worldwide assessment of dalbavancin activity and spectrum against over 6,000 clinical isolates,” Diagn. Microbiol. Infect. Dis., Vol. 48, pp.137-143, 2004.
[11] A. Coates, Y. Hu, R. Bax, and C. Page, “The future challenges facing the development of new antimicrobial drugs,” Nat. Rev. Drug Discov., Vol. 1, pp. 895-901, 2002.
[12] Forther JD, Lyon DY, Sayes CM, Boyd AM, Falkner JC, Hotze EM, Alemany et al. C-60 in water: Nanocrystal formation and microbial response. Environ Sci Technol 2005;39(11):430716.
[13] Herrera M. Carrion P, Baca P, Liebana J, Castillo A. In vitro antibacterial activity of glass-ionomer cements Microbios 2001;104(409): 141-48.
[14] A. Fujishima, K Honda, Nature 238, 37 (1972).
[15] Jha AK, Prasad K, Kulkarni AR. Colloids Surf B Biointerfaces 2009;71:226.
[16] Sungkaworn T, Triampo W, nalakarn P, Triampo D, tang IM, Lenbury Y, et al. The effects of TiO2 nanoparticles on tumor cells colonies: fractal dimension and morphological properties, Int J biomed Sci 2007;2(1):67-74.
[17] Rajh T, Chen LX, Lukas K, Liu T, Thurnauer MC, Tiede DM. J Phys Chem B 2002;106: 10543.
[18] Zhang H, Chen G. Potent antibacterial activities of Ag/TiO2 nanocomposite powders synthesized by a one-potsol-gel method. Environ Sci Technol 2009;34(8):2905-10.
[19] Holt KB, Bard AJ. Interaction of silver (1) ions with the respiratory chain of Escherichia coli: An electrochemical and scanning electrochemical microscopy of micromolar Ag. Biochemistry 2005; 44(39): 13214-23.
[20] Qin WT, Shen Y, Zhang HF, et al. Application and Research of Modified nano Oxide in Antibacterial Finishing of Fabric. Dyeing, 2005; 31 (21): 4-6.
[1] G. Zhao and S. E. Stevens, “Multiple Parameters for the Comprehensive Evaluation of the Susceptibility of Escherichia coli to the Silver Ion,” BioMetals, Vol. 11, pp. 27-32, 1998.
[2] J.H. Crabtree, R.J. Burchette, R.A. Siddiqi, I.T. Huen, L.L. Hadnott, and A. Fishman, “The efficacy of silver-ion implanted catheters in reducing peritoneal dialysis-related infections,” Perit Dial Int., Vol.23, pp.368-74, 2003.
[3] A. Krolikowska, A. Kudelski, A. Michota, and J. Bukowska, “SERS Studies on the Structure of Thioglycolic Acid Monolayers on Silver and Gold,” Surf. Sci., Vol. 532, pp. 227-232, 2003.
[4] M. Catauro, M.G. Raucci, F.D. De Gaaetano, and A. Marotta, “Antibacterial and bioactive silver-containing Na2O.CaO.2SiO2 glass prepared by sol-gel method,” J. Mater. Sci. Mater. Med., Vol. 15, pp.831-837, 2004.
[5] Sun D, Meng TT, Loong H, Hwa TJ. Water Sci Technol 2004;49:103.
[6] Gelis C, Girard S, Mavon A, Delverdier M, Pailous N, Vicendo P. Photomed 2003;19: 242.
[7] Joerger R, Klaus T, Granqvist CG. Adv Mater 2000;12:407.
[8] Tolles WM, Rath BB. Curr Sci 2003;85:1746.
[9] X. Gao, Y. Cui, R.M. Levenson, L.W. Chung, and S.Nie,“In vivo cancer targeting and imaging with semiconductor quantum dots,”Nature Biotechnology, Vol. 8pp. 969-76,2004.
[10] J.M. Streit, T.R. Fritsche, H.S. Sader, and R.N. Jones, “Worldwide assessment of dalbavancin activity and spectrum against over 6,000 clinical isolates,” Diagn. Microbiol. Infect. Dis., Vol. 48, pp.137-143, 2004.
[11] A. Coates, Y. Hu, R. Bax, and C. Page, “The future challenges facing the development of new antimicrobial drugs,” Nat. Rev. Drug Discov., Vol. 1, pp. 895-901, 2002.
[12] Forther JD, Lyon DY, Sayes CM, Boyd AM, Falkner JC, Hotze EM, Alemany et al. C-60 in water: Nanocrystal formation and microbial response. Environ Sci Technol 2005;39(11):430716.
[13] Herrera M. Carrion P, Baca P, Liebana J, Castillo A. In vitro antibacterial activity of glass-ionomer cements Microbios 2001;104(409): 141-48.
[14] A. Fujishima, K Honda, Nature 238, 37 (1972).
[15] Jha AK, Prasad K, Kulkarni AR. Colloids Surf B Biointerfaces 2009;71:226.
[16] Sungkaworn T, Triampo W, nalakarn P, Triampo D, tang IM, Lenbury Y, et al. The effects of TiO2 nanoparticles on tumor cells colonies: fractal dimension and morphological properties, Int J biomed Sci 2007;2(1):67-74.
[17] Rajh T, Chen LX, Lukas K, Liu T, Thurnauer MC, Tiede DM. J Phys Chem B 2002;106: 10543.
[18] Zhang H, Chen G. Potent antibacterial activities of Ag/TiO2 nanocomposite powders synthesized by a one-potsol-gel method. Environ Sci Technol 2009;34(8):2905-10.
[19] Holt KB, Bard AJ. Interaction of silver (1) ions with the respiratory chain of Escherichia coli: An electrochemical and scanning electrochemical microscopy of micromolar Ag. Biochemistry 2005; 44(39): 13214-23.
[20] Qin WT, Shen Y, Zhang HF, et al. Application and Research of Modified nano Oxide in Antibacterial Finishing of Fabric. Dyeing, 2005; 31 (21): 4-6.
@article{"International Journal of Chemical, Materials and Biomolecular Sciences:72800", author = "Prachi Singh", title = "Biosynthesis of Titanium Dioxide Nanoparticles and Their Antibacterial Property", abstract = "This paper presents a low-cost, eco-friendly and reproducible microbe mediated biosynthesis of TiO2 nanoparticles. TiO2 nanoparticles synthesized using the bacterium, Bacillus subtilis, from titanium as a precursor, were confirmed by TEM analysis. The morphological characteristics state spherical shape, with the size of individual or aggregate nanoparticles, around 30-40 nm. Microbial resistance represents a challenge for the scientific community to develop new bioactive compounds. Here, the antibacterial effect of TiO2 nanoparticles on Escherichia coli was investigated, which was confirmed by CFU (Colony-forming unit). Further, growth curve study of E. coli Hb101 in the presence and absence of TiO2 nanoparticles was done. Optical density decrease was observed with the increase in the concentration of TiO2. It could be attributed to the inactivation of cellular enzymes and DNA by binding to electron-donating groups such as carboxylates, amides, indoles, hydroxyls, thiols, etc. which cause little pores in bacterial cell walls, leading to increased permeability and cell death. This justifies that TiO2 nanoparticles have efficient antibacterial effect and have potential to be used as an antibacterial agent for different purposes.
", keywords = "Antibacterial effect, CFU, Escherichia coli Hb101, growth curve, TEM, TiO2 nanoparticle, toxicity, UV-Vis.", volume = "10", number = "2", pages = "275-4", }
