Abstract: To identify an endothelial cell-specific promoter suitable for vascular-specific targeting, we tested five promoters in vitro--Tie2SE, Tie2LE, ICAM2, Flt-1 and vWF--for promoter activity and specificity in endothelial cells, smooth muscle cells and non-vascular resident cells as well as tissues. These promoters, except for vWF, exhibited good endothelial activity and specificity in vitro. In a syngenic heart transplantation model, the ICAM2 promoter was variably functional in coronary endothelial cells of donor hearts. Thus, the ICAM2, Flt-1, Tie2SE and Tie2LE promoters hold promise for endothelial-specific targeting, but in vitro expression may not predict in vivo expression.
Abstract: The aged are faced with increasing risk for falls. The
aged have the easily fragile bones than others. When falls have
occurred, it is important to detect this emergency state because such
events often lead to more serious illness or even death. A
implementation of PDA system, for detection of emergency situation,
was developed using 3-axis accelerometer in this paper as follows.
The signals were acquired from the 3-axis accelerometer, and then
transmitted to the PDA through Bluetooth module. This system can
classify the human activity, and also detect the emergency state like
falls. When the fall occurs, the system generates the alarm on the
PDA. If a subject does not respond to the alarm, the system determines
whether the current situation is an emergency state or not, and then
sends some information to the emergency center in the case of urgent
situation. Three different studies were conducted on 12 experimental
subjects, with results indicating a good accuracy. The first study was
performed to detect the posture change of human daily activity. The
second study was performed to detect the correct direction of fall. The
third study was conducted to check the classification of the daily
physical activity. Each test was lasted at least 1 min. in third study.
The output of acceleration signal was compared and evaluated by
changing a various posture after attaching a 3-axis accelerometer
module on the chest. The newly developed system has some important
features such as portability, convenience and low cost. One of the
main advantages of this system is that it is available at home
healthcare environment. Another important feature lies in low cost to
manufacture device. The implemented system can detect the fall
accurately, so will be widely used in emergency situation.
Abstract: Diabetes mellitus (DM) is frequently characterized by
autonomic nervous dysfunction. Analysis of heart rate variability
(HRV) has become a popular noninvasive tool for assessing the
activities of autonomic nervous system (ANS). In this paper, changes
in ANS activity are quantified by means of frequency and time
domain analysis of R-R interval variability. Electrocardiograms
(ECG) of 16 patients suffering from DM and of 16 healthy volunteers
were recorded. Frequency domain analysis of extracted normal to
normal interval (NN interval) data indicates significant difference in
very low frequency (VLF) power, low frequency (LF) power and
high frequency (HF) power, between the DM patients and control
group. Time domain measures, standard deviation of NN interval
(SDNN), root mean square of successive NN interval differences
(RMSSD), successive NN intervals differing more than 50 ms (NN50
Count), percentage value of NN50 count (pNN50), HRV triangular
index and triangular interpolation of NN intervals (TINN) also show
significant difference between the DM patients and control group.
Abstract: TELMES project aims to develop a securized
multimedia system devoted to medical consultation teleservices. It
will be finalized with a pilot system for a regional telecenters
network that connects local telecenters, having as support
multimedia platforms. This network will enable the implementation
of complex medical teleservices (teleconsulations, telemonitoring,
homecare, urgency medicine, etc.) for a broader range of patients
and medical professionals, mainly for family doctors and those
people living in rural or isolated regions. Thus, a multimedia,
scalable network, based on modern IT&C paradigms, will result. It
will gather two inter-connected regional telecenters, in Iaşi and
Piteşti, Romania, each of them also permitting local connections of
hospitals, diagnostic and treatment centers, as well as local networks
of family doctors, patients, even educational entities. As
communications infrastructure, we aim to develop a combined fixmobile-
internet (broadband) links. Other possible communication
environments will be GSM/GPRS/3G and radio waves. The
electrocardiogram (ECG) acquisition, internet transmission and
local analysis, using embedded technologies, was already
successfully done for patients- telemonitoring.
Abstract: Chest pain is one of the most prevalent complaints
among adults that cause the people to attend to medical centers. The
aim was to determine the prevalence and risk factors of chest pain
among over 30 years old people in Tehran. In this cross-sectional
study, 787 adults took part from Apr 2005 until Apr 2006. The
sampling method was random cluster sampling and there were 25
clusters. In each cluster, interviews were performed with 32 over 30
years old, people lived in those houses. In cases with chest pain, extra
questions asked. The prevalence of CP was 9% (71 cases). Of them
21 cases (6.5%) were in 41-60 year age ranges and the remainders
were over 61 year old. 19 cases (26.8%) mentioned CP in resting
state and all of the cases had exertion onset CP. The CP duration was
10 minutes or less in all of the cases and in most of them (84.5%), the
location of pain mentioned left anterior part of chest, left anterior part
of sternum and or left arm. There was positive history of myocardial
infarction in 12 cases (17%). There was significant relation between
CP and age, sex and between history of myocardial infarction and
marital state of study people. Our results are similar to other studies-
results in most parts, however it is necessary to perform
supplementary tests and follow up studies to differentiate between
cardiac and non-cardiac CP exactly.
Abstract: In this paper, a mathematical model of human immunodeficiency
virus (HIV) is utilized and an optimization problem is
proposed, with the final goal of implementing an optimal 900-day
structured treatment interruption (STI) protocol. Two type of commonly
used drugs in highly active antiretroviral therapy (HAART),
reverse transcriptase inhibitors (RTI) and protease inhibitors (PI), are
considered. In order to solving the proposed optimization problem an
adaptive memetic algorithm with population management (AMAPM)
is proposed. The AMAPM uses a distance measure to control the
diversity of population in genotype space and thus preventing the
stagnation and premature convergence. Moreover, the AMAPM uses
diversity parameter in phenotype space to dynamically set the population
size and the number of crossovers during the search process.
Three crossover operators diversify the population, simultaneously.
The progresses of crossover operators are utilized to set the number
of each crossover per generation. In order to escaping the local optima
and introducing the new search directions toward the global optima,
two local searchers assist the evolutionary process. In contrast to
traditional memetic algorithms, the activation of these local searchers
is not random and depends on both the diversity parameters in
genotype space and phenotype space. The capability of AMAPM in
finding optimal solutions compared with three popular metaheurestics
is introduced.
Abstract: Oral health is particular important to the hospitalized
patients with chronic schizophrenia for an extreme high potential of the respiratory infections. Due to the degeneration of physical capability, patients of this kind typically fall dependent in the activity of daily living (ADL). A very high percentage of patients had dental
problems of which mostly could be easily avoid by easy regular tooth brushing. Purpose of the project is to develop a mechanism in helping the schizophrenia patients in rebuilding a tooth-cleaning habit. The
project observed and evaluated the tooth-cleaning behavior of 100
male patients in a psychiatric hospital, and found the majority of them
ignored such an activity in a three-month period of time. In the meantime, the primary care-givers were not aware or not convinced
the importance of such a need of dental hygiene, and thus few if any
tooth cleaning training or knowledge on dental hygiene were given to
the patients. The project then developed a program based on the numerous observations and discussions. The improvement program
included patients- group education, care-givers- training, and a
tool-kit for tooth-brush holding was erected. The project launched
with some incentive package. The outcomes were encouraging with
87% of the patients had rebuilt their tooth-brushing habits against
previous 22%, and the tooth cleaning kits were 100% kept against 22%
in the past. This project had significantly improved the oral health of
the patients. The project, included the procedure and the tool-kit
holder specific for this purpose, was a good examples for psychiatric
hospitals.
Abstract: Breast cancer detection techniques have been reported
to aid radiologists in analyzing mammograms. We note that most
techniques are performed on uncompressed digital mammograms.
Mammogram images are huge in size necessitating the use of
compression to reduce storage/transmission requirements. In this
paper, we present an algorithm for the detection of
microcalcifications in the JPEG2000 domain. The algorithm is based
on the statistical properties of the wavelet transform that the
JPEG2000 coder employs. Simulation results were carried out at
different compression ratios. The sensitivity of this algorithm ranges
from 92% with a false positive rate of 4.7 down to 66% with a false
positive rate of 2.1 using lossless compression and lossy compression
at a compression ratio of 100:1, respectively.
Abstract: Hepatocellular carcinoma, also called hepatoma, most
commonly appears in a patient with chronic viral hepatitis. In
patients with a higher suspicion of HCC, such as small or subtle
rising of serum enzymes levels, the best method of diagnosis
involves a CT scan of the abdomen, but only at high cost. The aim of
this study was to increase the ability of the physician to early detect
HCC, using a probabilistic neural network-based approach, in order
to save time and hospital resources.
Abstract: In ubiqutious healthcare environment, user's health data are transfered to the remote healthcare server by the user's wearable system or mobile phone. These collected user's health data should be managed and analyzed in the healthcare server, so that care giver or user can monitor user's physiological state. In this paper, we designed and developed the intelligent Healthcare Server to manage the user's health data using CDSS and ontology. Our system can analyze user's health data semantically using CDSS and ontology, and report the result of user's physiological raw data to the user and care giver.
Abstract: The spores of entomopathogenic fungi, Beauveria bassiana was evaluated for their compatibility with four surfactants; SDS (sodium dodyl sulphate) and CABS-65 (calcium alkyl benzene sulphonate), Tween 20 (polyethylene sorbitan monolaureate) and Tween 80 (polyoxyethylene sorbitan monoleate) at six different concentrations (0.1%, 0.5%, 1%, 2.5%, 5% and 10%). Incubated spores showed decrease in concentrations due to conversion of spores to hyphae. The maximum germination recorded in 72 h incubated spores varied with surfactant concentration at 49-68% (SDS), 39- 53% (CABS), 78-92% (Tween 80) and 80-92% (Tween 20), while the optimal surfactant concentration for spore germination was found to be 2.5-5%. The surfactant effect on spores was more pronounced with SDS and CABS-65, where significant deterioration and loss in viability of the incubated spores was observed. The effect of Tween 20 and Tween 80 were comparatively less inhibiting. The results of the study would help in surfactant selection for B. bassiana emulsion preparation.
Abstract: The regulatory competence of blood glucose homeostasis might determine the degree of academic performance. The aim of this study was to produce a model of students' alcohol use based on glucose homeostasis control and cognitive functions that might define the pathogenetic mechanism of alcohol's effect on academic performance. The study took six hours and thirty minutes on fasting, involving thirteen male students. Disturbances in cognitive functions, precisely a decrease in the effectiveness of active attention and a faster development of fatigue after four to six hours of mental work in alcohol users, compared to abstainers was statistically proven. These disturbances in alcohol users were retained even after seven to ten days of moderate alcohol use and might be the reason for the low academic performances among students who use alcoholic beverages.
Abstract: Diagnosis can be achieved by building a model of a
certain organ under surveillance and comparing it with the real time
physiological measurements taken from the patient. This paper deals
with the presentation of the benefits of using Data Mining techniques
in the computer-aided diagnosis (CAD), focusing on the cancer
detection, in order to help doctors to make optimal decisions quickly
and accurately. In the field of the noninvasive diagnosis techniques,
the endoscopic ultrasound elastography (EUSE) is a recent elasticity
imaging technique, allowing characterizing the difference between
malignant and benign tumors. Digitalizing and summarizing the main
EUSE sample movies features in a vector form concern with the use
of the exploratory data analysis (EDA). Neural networks are then
trained on the corresponding EUSE sample movies vector input in
such a way that these intelligent systems are able to offer a very
precise and objective diagnosis, discriminating between benign and
malignant tumors. A concrete application of these Data Mining
techniques illustrates the suitability and the reliability of this
methodology in CAD.
Abstract: The prevalence of non organic constipation differs
from country to country and the reliability of the estimate rates is
uncertain. Moreover, the clinical relevance of subdividing the
heterogeneous functional constipation disorders into pre-defined
subgroups is largely unknown.. Aim: to estimate the prevalence of
constipation in a population-based sample and determine whether
clinical subgroups can be identified. An age and gender stratified
sample population from 5 Italian cities was evaluated using a
previously validated questionnaire. Data mining by cluster analysis
was used to determine constipation subgroups. Results: 1,500
complete interviews were obtained from 2,083 contacted households
(72%). Self-reported constipation correlated poorly with symptombased
constipation found in 496 subjects (33.1%). Cluster analysis
identified four constipation subgroups which correlated to subgroups
identified according to pre-defined symptom criteria. Significant
differences in socio-demographics and lifestyle were observed
among subgroups.
Abstract: Snake bite cases in Malaysia most often involve the
species Naja-naja and Calloselasma rhodostoma. In keeping with the
need for a rapid snake venom detection kit in a clinical setting, plate
and dot-ELISA test for the venoms of Naja-naja sumatrana,
Calloselasma rhodostoma and the cobra venom fraction V antigen
was developed. Polyclonal antibodies were raised and further used to
prepare the reagents for the dot-ELISA test kit which was tested in
mice, rabbit and virtual human models. The newly developed dot-
ELISA kit was able to detect a minimum venom concentration of
244ng/ml with cross reactivity of one antibody type. The dot-ELISA
system was sensitive and specific for all three snake venom types in
all tested animal models. The lowest minimum venom concentration
detectable was in the rabbit model, 244ng/ml of the cobra venom
fraction V antigen. The highest minimum venom concentration was
in mice, 1953ng/ml against a multitude of venoms. The developed
dot-ELISA system for the detection of three snake venom types was
successful with a sensitivity of 95.8% and specificity of 97.9%.
Abstract: The major objective of this paper is to introduce a new method to select genes from DNA microarray data. As criterion to select genes we suggest to measure the local changes in the correlation graph of each gene and to select those genes whose local changes are largest. More precisely, we calculate the correlation networks from DNA microarray data of cervical cancer whereas each network represents a tissue of a certain tumor stage and each node in the network represents a gene. From these networks we extract one tree for each gene by a local decomposition of the correlation network. The interpretation of a tree is that it represents the n-nearest neighbor genes on the n-th level of a tree, measured by the Dijkstra distance, and, hence, gives the local embedding of a gene within the correlation network. For the obtained trees we measure the pairwise similarity between trees rooted by the same gene from normal to cancerous tissues. This evaluates the modification of the tree topology due to tumor progression. Finally, we rank the obtained similarity values from all tissue comparisons and select the top ranked genes. For these genes the local neighborhood in the correlation networks changes most between normal and cancerous tissues. As a result we find that the top ranked genes are candidates suspected to be involved in tumor growth. This indicates that our method captures essential information from the underlying DNA microarray data of cervical cancer.
Abstract: The overall penumbra is usually defined as the
distance, p20–80, separating the 20% and 80% of the dose on the beam axis at the depth of interest. This overall penumbra accounts
also for the fact that some photons emitted by the distal parts of the source are only partially attenuated by the collimator. Medulloblastoma is the most common type of childhood brain tumor
and often spreads to the spine. Current guidelines call for surgery to remove as much of the tumor as possible, followed by radiation of the brain and spinal cord, and finally treatment with chemotherapy.
The purpose of this paper was to present results on an Uniformity of dose distribution in radiation fields surrounding the spine using film
dosimetry and comparison with 3D treatment planning software.
Abstract: Risk of infectious disease outbreaks is related to the
hygiene among the population. To assess the actual risks and modify
the relevant emergency procedures if necessary, a hygiene survey
was conducted among undergraduate students on the Rhodes
University campus. Soap was available to 10.5% and only 26.8% of
the study participants followed proper hygiene in relation to food
consumption. This combination increases the risk of infectious
disease outbreaks at the campus. Around 83.6% were willing to wash
their hands if soap was provided. Procurement and availability of
soap in undergraduate residences on campus should be improved, as
the total cost is estimated at only 2000 USD per annum. Awareness
campaigns about food-related hygiene and the need for regular handwashing
with soap should be run among Rhodes University students.
If successful, rates of respiratory and hygiene-related diseases will be
decreased and emergency health management simplified.
Abstract: Purpose: Planning and dosimetry of different VMAT algorithms (SmartArc, Ergo++, Autobeam) is compared with IMRT for Head and Neck Cancer patients. Modelling was performed to rule out the causes of discrepancies between planned and delivered dose. Methods: Five HNC patients previously treated with IMRT were re-planned with SmartArc (SA), Ergo++ and Autobeam. Plans were compared with each other and against IMRT and evaluated using DVHs for PTVs and OARs, delivery time, monitor units (MU) and dosimetric accuracy. Modelling of control point (CP) spacing, Leaf-end Separation and MLC/Aperture shape was performed to rule out causes of discrepancies between planned and delivered doses. Additionally estimated arc delivery times, overall plan generation times and effect of CP spacing and number of arcs on plan generation times were recorded. Results: Single arc SmartArc plans (SA4d) were generally better than IMRT and double arc plans (SA2Arcs) in terms of homogeneity and target coverage. Double arc plans seemed to have a positive role in achieving improved Conformity Index (CI) and better sparing of some Organs at Risk (OARs) compared to Step and Shoot IMRT (ss-IMRT) and SA4d. Overall Ergo++ plans achieved best CI for both PTVs. Dosimetric validation of all VMAT plans without modelling was found to be lower than ss-IMRT. Total MUs required for delivery were on average 19%, 30%, 10.6% and 6.5% lower than ss-IMRT for SA4d, SA2d (Single arc with 20 Gantry Spacing), SA2Arcs and Autobeam plans respectively. Autobeam was most efficient in terms of actual treatment delivery times whereas Ergo++ plans took longest to deliver. Conclusion: Overall SA single arc plans on average achieved best target coverage and homogeneity for both PTVs. SA2Arc plans showed improved CI and some OARs sparing. Very good dosimetric results were achieved with modelling. Ergo++ plans achieved best CI. Autobeam resulted in fastest treatment delivery times.