Abstract: In this paper we have proposed a methodology to
develop an amperometric biosensor for the analysis of glucose
concentration using a simple microcontroller based data acquisition
system. The work involves the development of Detachable
Membrane Unit (enzyme based biomembrane) with immobilized
glucose oxidase on the membrane and interfacing the same to the
signal conditioning system. The current generated by the biosensor
for different glucose concentrations was signal conditioned, then
acquired and computed by a simple AT89C51-microcontroller. The
optimum operating parameters for the better performance were found
and reported. The detailed performance evaluation of the biosensor
has been carried out. The proposed microcontroller based biosensor
system has the sensitivity of 0.04V/g/dl, with a resolution of
50mg/dl. It has exhibited very good inter day stability observed up to
30 days. Comparing to the reference method such as HPLC, the
accuracy of the proposed biosensor system is well within ± 1.5%.
The system can be used for real time analysis of glucose
concentration in the field such as, food and fermentation and clinical
(In-Vitro) applications.