Abstract: Production of biogas from bakery waste was enhanced
by additional bacterial cell. This study was divided into 2 steps. First
step, grease waste from bakery industry-s grease trap was initially
degraded by Pseudomonas aeruginosa. The concentration of byproduct,
especially glycerol, was determined and found that glycerol
concentration increased from 12.83% to 48.10%. Secondary step, 3
biodigesters were set up in 3 different substrates: non-degraded waste
as substrate in first biodigester, degraded waste as substrate in
secondary biodigester, and degraded waste mixed with swine manure
in ratio 1:1 as substrate in third biodigester. The highest
concentration of biogas was found in third biodigester that was
44.33% of methane and 63.71% of carbon dioxide. The lower
concentration at 24.90% of methane and 18.98% of carbon dioxide
was exhibited in secondary biodigester whereas the lowest was found
in non-degraded waste biodigester. It was demonstrated that the
biogas production was greatly increased with the initial grease waste
degradation by Pseudomonas aeruginosa.